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. 1983 May;40(2):629–632. doi: 10.1128/iai.40.2.629-632.1983

Identification of the major antigenic determinants on lipoglycans from Acholeplasma granularum and Acholeplasma axanthum.

T H Al-Samarrai, P F Smith, R J Lynn
PMCID: PMC264900  PMID: 6188700

Abstract

The major antigenic determinants of the lipoglycans from Acholeplasma granularum and Acholeplasma axanthum were found to be the oligosaccharide sequences Glcp(beta 1 leads to 2)-Glcp(alpha 1 leads to 4)-Glcp and Glcp(beta 1 leads to 2)-Glcp(beta 1 leads to 2)-Glcp, respectively. The disaccharides sophorose and maltose inhibited hemagglutination by specific antiserum of sheep erythrocytes coated with lipoglycan from A. granularum. Only sophorose possessed this capacity with the lipoglycan from A. axanthum. Periodate oxidation destroyed the ability of both lipoglycans to interact with specific antibody, a result compatible with structural data on the lipoglycans.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Al-Samarrai T. H., Smith P. F., Lynn R. J. Isolation and characterization of the sheep erythrocyte receptor for acholeplasmal lipoglycans. Infect Immun. 1982 Dec;38(3):1078–1087. doi: 10.1128/iai.38.3.1078-1087.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chan W. W., Schutt H., Brand K. Active subunits of transaldolase bound to sepharose. Eur J Biochem. 1973 Dec 17;40(2):533–541. doi: 10.1111/j.1432-1033.1973.tb03224.x. [DOI] [PubMed] [Google Scholar]
  3. Furukawa K., Kochibe N., Takizawa H., Iseki S. Immunochemical studies on bacterial blood group substances. VI. Structures of A-active oligosaccharides isolated from Salmonella riogrande lipopolysaccharide. Jpn J Microbiol. 1972 May;16(3):199–204. doi: 10.1111/j.1348-0421.1972.tb00649.x. [DOI] [PubMed] [Google Scholar]
  4. Lynn R. J., Guidici P. A., Hollemweguer E. J., Smith P. F. Humoral immune response of rabbits to acholeplasmal lipoglycans. Infect Immun. 1980 Sep;29(3):926–933. doi: 10.1128/iai.29.3.926-933.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. MCCARTY M. Variation in the group-specific carbohydrate of group A streptococci. II. Studies on the chemical basis for serological specificity of the carbohydrates. J Exp Med. 1956 Nov 1;104(5):629–643. doi: 10.1084/jem.104.5.629. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. MORGAN W. T. J., WATKINS W. M. The inhibition of the haemagglutinins in plant seeds by human blood group substances and simple sugars. Br J Exp Pathol. 1953 Feb;34(1):94–103. [PMC free article] [PubMed] [Google Scholar]
  7. McCARTY M. Further studies on the chemical basis for serological specificity of Group A streptococcal carbohydrate. J Exp Med. 1958 Sep 1;108(3):311–323. doi: 10.1084/jem.108.3.311. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Smith P. F. Homogeneity of lipopolysaccharides from Acholeplasma. J Bacteriol. 1977 Apr;130(1):393–398. doi: 10.1128/jb.130.1.393-398.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Smith P. F., Langworthy T. A., Mayberry W. R. Distribution and composition of lipopolysaccharides from mycoplasmas. J Bacteriol. 1976 Mar;125(3):916–922. doi: 10.1128/jb.125.3.916-922.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Smith P. F. Structure of the oligosaccharide chain of lipoglycan from Acholeplasma granularum. Biochim Biophys Acta. 1981 Jul 24;665(1):92–99. doi: 10.1016/0005-2760(81)90237-x. [DOI] [PubMed] [Google Scholar]
  11. Sugiyama T., Smith P. F., Langworthy T. A., Mayberry W. R. Immunological analysis of glycolipids and lipopolysaccharides derived from various mycoplasmas. Infect Immun. 1974 Dec;10(6):1273–1279. doi: 10.1128/iai.10.6.1273-1279.1974. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. WHEAT R. W., DORSCH C., GODOY G. OCCURRENCE OF PYRUVIC ACID IN THE CAPSULAR POLYSACCHARIDE OF KLEBSIELLA RHINOSCLEROMATIS. J Bacteriol. 1965 Feb;89:539–539. doi: 10.1128/jb.89.2.539-539.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]

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