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. Author manuscript; available in PMC: 2009 Nov 11.
Published in final edited form as: Biochemistry. 2008 Oct 18;47(45):11894–11899. doi: 10.1021/bi801211t

Figure 4.

Figure 4

Rates of [35S]PAPS ⇋ [35S]P isotopic exchange versus [S]0/[P]0 ratios. Concentrations of PAPS and PAP are 10 ∝M and 2 ∝M, respectively. Concentration of added [35S]PAPS pulse is 0.05 ∝M; 7.5 ∝g SULT1A1 in 50 ∝l phosphate buffer (final concentration: 50 mM, pH 6.2) at 37°C.