Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Jan;30(1):36–40. doi: 10.1128/jcm.30.1.36-40.1992

Detection of human immunodeficiency virus type 1 infection in young pediatric patients by using polymerase chain reaction and biotinylated probes.

C D Brandt 1, T A Rakusan 1, A V Sison 1, S H Josephs 1, E S Saxena 1, K D Herzog 1, R H Parrott 1, J L Sever 1
PMCID: PMC264992  PMID: 1734067

Abstract

Polymerase chain reaction (PCR) testing using up to four primer pairs and biotinylated probes was 97.9% sensitive (188 of 192 specimens positive) and 100% specific (267 of 267 specimens negative) for detecting the presence or absence of human immunodeficiency virus (HIV) DNA in peripheral blood mononuclear cells from pediatric patients whose HIV status has been confirmed. SK38/39 and SK145/150 were the most sensitive primer pairs, respectively detecting HIV DNA in 95.6 and 95.9% of peripheral blood mononuclear cell specimens from HIV-infected children and collectively detecting all adequately tested PCR-positive specimens. Primer pairs SK29/30 and SK68/69 respectively detected HIV DNA in only 76.4 and 76.6% of HIV-positive specimens. Among infants born to HIV-seropositive mothers, 30 who subsequently were confirmed to be infected were sampled when they were less than or equal to 6 months of age; in all but one infant, HIV DNA was found in the first specimen collected. Among the nine youngest infected infants tested, all were PCR positive by 38 days of age. PCR methods thus have reliably detected vertically transmitted HIV infection early in life.

Full text

PDF
36

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andiman W. A., Simpson B. J., Olson B., Dember L., Silva T. J., Miller G. Rate of transmission of human immunodeficiency virus type 1 infection from mother to child and short-term outcome of neonatal infection. Results of a prospective cohort study. Am J Dis Child. 1990 Jul;144(7):758–766. doi: 10.1001/archpedi.1990.02150310026020. [DOI] [PubMed] [Google Scholar]
  2. Blanche S., Rouzioux C., Moscato M. L., Veber F., Mayaux M. J., Jacomet C., Tricoire J., Deville A., Vial M., Firtion G. A prospective study of infants born to women seropositive for human immunodeficiency virus type 1. HIV Infection in Newborns French Collaborative Study Group. N Engl J Med. 1989 Jun 22;320(25):1643–1648. doi: 10.1056/NEJM198906223202502. [DOI] [PubMed] [Google Scholar]
  3. Candotti D., Jung M., Kerouedan D., Rosenheim M., Gentilini M., M'Pele P., Huraux J. M., Agut H. Genetic variability affects the detection of HIV by polymerase chain reaction. AIDS. 1991 Aug;5(8):1003–1007. doi: 10.1097/00002030-199108000-00012. [DOI] [PubMed] [Google Scholar]
  4. Chadwick E. G., Yogev R., Kwok S., Sninsky J. J., Kellogg D. E., Wolinsky S. M. Enzymatic amplification of the human immunodeficiency virus in peripheral blood mononuclear cells from pediatric patients. J Infect Dis. 1989 Dec;160(6):954–959. doi: 10.1093/infdis/160.6.954. [DOI] [PubMed] [Google Scholar]
  5. De Rossi A., Ades A. E., Mammano F., Del Mistro A., Amadori A., Giaquinto C., Chieco-Bianchi L. Antigen detection, virus culture, polymerase chain reaction, and in vitro antibody production in the diagnosis of vertically transmitted HIV-1 infection. AIDS. 1991 Jan;5(1):15–20. doi: 10.1097/00002030-199101000-00002. [DOI] [PubMed] [Google Scholar]
  6. De Rossi A., Amadori A., Chieco-Bianchi L., Giacquinto C., Zacchello F., Buchbinder A., Wong-Staal F., Gallo R. C., Peckham C. S. Polymerase chain reaction and in-vitro antibody production for early diagnosis of paediatric HIV infection. Lancet. 1988 Jul 30;2(8605):278–278. doi: 10.1016/s0140-6736(88)92565-2. [DOI] [PubMed] [Google Scholar]
  7. Edwards J. R., Ulrich P. P., Weintrub P. S., Cowan M. J., Levy J. A., Wara D. W., Vyas G. N. Polymerase chain reaction compared with concurrent viral cultures for rapid identification of human immunodeficiency virus infection among high-risk infants and children. J Pediatr. 1989 Aug;115(2):200–203. doi: 10.1016/s0022-3476(89)80065-4. [DOI] [PubMed] [Google Scholar]
  8. Hewlett I. K., Laurian Y., Epstein J., Hawthorne C. A., Ruta M., Allain J. P. Assessment by gene amplification and serological markers of transmission of HIV-1 from hemophiliacs to their sexual partners and secondarily to their children. J Acquir Immune Defic Syndr. 1990;3(7):714–720. [PubMed] [Google Scholar]
  9. Holodniy M., Kim S., Katzenstein D., Konrad M., Groves E., Merigan T. C. Inhibition of human immunodeficiency virus gene amplification by heparin. J Clin Microbiol. 1991 Apr;29(4):676–679. doi: 10.1128/jcm.29.4.676-679.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Husson R. N., Comeau A. M., Hoff R. Diagnosis of human immunodeficiency virus infection in infants and children. Pediatrics. 1990 Jul;86(1):1–10. [PubMed] [Google Scholar]
  11. Jackson J. B., Balfour H. H., Jr Practical diagnostic testing for human immunodeficiency virus. Clin Microbiol Rev. 1988 Jan;1(1):124–138. doi: 10.1128/cmr.1.1.124. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Jackson J. B., Kwok S. Y., Sninsky J. J., Hopsicker J. S., Sannerud K. J., Rhame F. S., Henry K., Simpson M., Balfour H. H., Jr Human immunodeficiency virus type 1 detected in all seropositive symptomatic and asymptomatic individuals. J Clin Microbiol. 1990 Jan;28(1):16–19. doi: 10.1128/jcm.28.1.16-19.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Krivine A., Yakudima A., Le May M., Pena-Cruz V., Huang A. S., McIntosh K. A comparative study of virus isolation, polymerase chain reaction, and antigen detection in children of mothers infected with human immunodeficiency virus. J Pediatr. 1990 Mar;116(3):372–376. doi: 10.1016/s0022-3476(05)82823-9. [DOI] [PubMed] [Google Scholar]
  14. Laure F., Courgnaud V., Rouzioux C., Blanche S., Veber F., Burgard M., Jacomet C., Griscelli C., Brechot C. Detection of HIV1 DNA in infants and children by means of the polymerase chain reaction. Lancet. 1988 Sep 3;2(8610):538–541. doi: 10.1016/s0140-6736(88)92659-1. [DOI] [PubMed] [Google Scholar]
  15. Ou C. Y., Kwok S., Mitchell S. W., Mack D. H., Sninsky J. J., Krebs J. W., Feorino P., Warfield D., Schochetman G. DNA amplification for direct detection of HIV-1 in DNA of peripheral blood mononuclear cells. Science. 1988 Jan 15;239(4837):295–297. doi: 10.1126/science.3336784. [DOI] [PubMed] [Google Scholar]
  16. Oxtoby M. J. Perinatally acquired human immunodeficiency virus infection. Pediatr Infect Dis J. 1990 Sep;9(9):609–619. [PubMed] [Google Scholar]
  17. Paterlini P., Lallemant-Le Coeur S., Lallemant M., M'Pelé P., Dazza M. C., Terre S., Moncany M., Jourdain G., Courgnaud V., N'Zingoula S. Polymerase chain reaction for studies of mother to child transmission of HIV1 in Africa. J Med Virol. 1990 Jan;30(1):53–57. doi: 10.1002/jmv.1890300112. [DOI] [PubMed] [Google Scholar]
  18. Rakusan T. A., Parrott R. H., Sever J. L. Limitations in the laboratory diagnosis of vertically acquired HIV infection. J Acquir Immune Defic Syndr. 1991;4(2):116–121. [PubMed] [Google Scholar]
  19. Rogers M. F., Ou C. Y., Rayfield M., Thomas P. A., Schoenbaum E. E., Abrams E., Krasinski K., Selwyn P. A., Moore J., Kaul A. Use of the polymerase chain reaction for early detection of the proviral sequences of human immunodeficiency virus in infants born to seropositive mothers. New York City Collaborative Study of Maternal HIV Transmission and Montefiore Medical Center HIV Perinatal Transmission Study Group. N Engl J Med. 1989 Jun 22;320(25):1649–1654. doi: 10.1056/NEJM198906223202503. [DOI] [PubMed] [Google Scholar]
  20. Wages J. M., Jr, Hamdallah M., Calabro M. A., Fowler A. K., Oster C. N., Redfield R. R., Burke D. S. Clinical performance of a polymerase chain reaction testing algorithm for diagnosis of HIV-1 infection in peripheral blood mononuclear cells. J Med Virol. 1991 Jan;33(1):58–63. doi: 10.1002/jmv.1890330112. [DOI] [PubMed] [Google Scholar]
  21. Williams P., Simmonds P., Yap P. L., Balfe P., Bishop J., Brettle R., Hague R., Hargreaves D., Inglis J., Brown A. L. The polymerase chain reaction in the diagnosis of vertically transmitted HIV infection. AIDS. 1990 May;4(5):393–398. doi: 10.1097/00002030-199005000-00003. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES