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. 1992 Jan;30(1):255–258. doi: 10.1128/jcm.30.1.255-258.1992

Use of polymerase chain reaction for rapid diagnosis of tuberculosis.

D V Cousins 1, S D Wilton 1, B R Francis 1, B L Gow 1
PMCID: PMC265037  PMID: 1734065

Abstract

A DNA amplification assay using the polymerase chain reaction technique designed for the rapid identification of Mycobacterium bovis organisms was used to test 211 human mycobacterial isolates and 177 clinical specimens previously submitted for routine mycobacterial culture. The procedures described could be used by routine or specialist laboratories for identification of M. tuberculosis complex organisms in 4 h and/or as a rapid screening method for the direct detection of M. tuberculosis complex organisms in specimens.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Body B. A., Warren N. G., Spicer A., Henderson D., Chery M. Use of Gen-Probe and Bactec for rapid isolation and identification of mycobacteria. Correlation of probe results with growth index. Am J Clin Pathol. 1990 Mar;93(3):415–420. doi: 10.1093/ajcp/93.3.415. [DOI] [PubMed] [Google Scholar]
  2. Brisson-Noël A., Gicquel B., Lecossier D., Lévy-Frébault V., Nassif X., Hance A. J. Rapid diagnosis of tuberculosis by amplification of mycobacterial DNA in clinical samples. Lancet. 1989 Nov 4;2(8671):1069–1071. doi: 10.1016/s0140-6736(89)91082-9. [DOI] [PubMed] [Google Scholar]
  3. Cousins D. V., Wilton S. D., Francis B. R. Use of DNA amplification for the rapid identification of Mycobacterium bovis. Vet Microbiol. 1991 Apr;27(2):187–195. doi: 10.1016/0378-1135(91)90010-d. [DOI] [PubMed] [Google Scholar]
  4. Daniel T. M. The rapid diagnosis of tuberculosis: a selective review. J Lab Clin Med. 1990 Sep;116(3):277–282. [PubMed] [Google Scholar]
  5. Dawson D. J. Tuberculosis in Australia: an unfinished fight. Med J Aust. 1991 Jan 21;154(2):75–76. doi: 10.5694/j.1326-5377.1991.tb120983.x. [DOI] [PubMed] [Google Scholar]
  6. HOLST E., MITCHISON D. A., RADHAKRISHNA S. Examination of smears for tubercle bacilli by fluorescence microscopy. Indian J Med Res. 1959 Sep;47:495–499. [PubMed] [Google Scholar]
  7. Hance A. J., Grandchamp B., Lévy-Frébault V., Lecossier D., Rauzier J., Bocart D., Gicquel B. Detection and identification of mycobacteria by amplification of mycobacterial DNA. Mol Microbiol. 1989 Jul;3(7):843–849. doi: 10.1111/j.1365-2958.1989.tb00233.x. [DOI] [PubMed] [Google Scholar]
  8. Mullis K. B., Faloona F. A. Specific synthesis of DNA in vitro via a polymerase-catalyzed chain reaction. Methods Enzymol. 1987;155:335–350. doi: 10.1016/0076-6879(87)55023-6. [DOI] [PubMed] [Google Scholar]
  9. Oste C. Polymerase chain reaction. Biotechniques. 1988 Feb;6(2):162–167. [PubMed] [Google Scholar]
  10. Pao C. C., Yen T. S., You J. B., Maa J. S., Fiss E. H., Chang C. H. Detection and identification of Mycobacterium tuberculosis by DNA amplification. J Clin Microbiol. 1990 Sep;28(9):1877–1880. doi: 10.1128/jcm.28.9.1877-1880.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Park C. H., Hixon D. L., Ferguson C. B., Hall S. L., Risheim C. C., Cook C. B. Rapid recovery of mycobacteria from clinical specimens using automated radiometric technic. Am J Clin Pathol. 1984 Mar;81(3):341–345. doi: 10.1093/ajcp/81.3.341. [DOI] [PubMed] [Google Scholar]
  12. Peterson E. M., Lu R., Floyd C., Nakasone A., Friedly G., de la Maza L. M. Direct identification of Mycobacterium tuberculosis, Mycobacterium avium, and Mycobacterium intracellulare from amplified primary cultures in BACTEC media using DNA probes. J Clin Microbiol. 1989 Jul;27(7):1543–1547. doi: 10.1128/jcm.27.7.1543-1547.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Shankar P., Manjunath N., Mohan K. K., Prasad K., Behari M., Shriniwas, Ahuja G. K. Rapid diagnosis of tuberculous meningitis by polymerase chain reaction. Lancet. 1991 Jan 5;337(8732):5–7. doi: 10.1016/0140-6736(91)93328-7. [DOI] [PubMed] [Google Scholar]
  14. Shoemaker S. A., Fisher J. H., Scoggin C. H. Techniques of DNA hybridization detect small numbers of mycobacteria with no cross-hybridization with non-mycobacterial respiratory organisms. Am Rev Respir Dis. 1985 May;131(5):760–763. doi: 10.1164/arrd.1985.131.5.760. [DOI] [PubMed] [Google Scholar]
  15. Wood P. R., Ripper J., Radford A. J., Bundesen P. G., Rylatt D. B., Cottis L. E., John M., Plackett P. Production and characterization of monoclonal antibodies specific for Mycobacterium bovis. J Gen Microbiol. 1988 Sep;134(9):2599–2604. doi: 10.1099/00221287-134-9-2599. [DOI] [PubMed] [Google Scholar]

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