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. 1992 Feb;30(2):509–511. doi: 10.1128/jcm.30.2.509-511.1992

Local immune responses to the Campylobacter flagellin in acute Campylobacter gastrointestinal infection.

I Nachamkin 1, X H Yang 1
PMCID: PMC265089  PMID: 1371521

Abstract

Intestinal immunoglobulin A (IgA) anti-campylobacter flagellin antibodies were measured by an enzyme-linked immunosorbent assay in patients with and without campylobacter infections. The level of fecal IgA antiflagellin antibodies was significantly higher in patients than in controls, but only 12 of 29 patients (41.4%) with a campylobacter infection had detectable IgA antibodies against flagellin (optical density, greater than or equal to 0.100). Further testing of fecal IgA antibodies from 10 patients with homologous and heterologous purified flagellins showed strain specificity in 1 patient. Cross-reactivity of fecal IgA antibodies with heterologous flagellins did not correlate with the Lior serotype of the isolate.

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Selected References

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  1. Abimiku A. G., Dolby J. M. Cross-protection of infant mice against intestinal colonisation by Campylobacter jejuni: importance of heat-labile serotyping (Lior) antigens. J Med Microbiol. 1988 Aug;26(4):265–268. doi: 10.1099/00222615-26-4-265. [DOI] [PubMed] [Google Scholar]
  2. Aguero-Rosenfeld M. E., Yang X. H., Nachamkin I. Infection of adult Syrian hamsters with flagellar variants of Campylobacter jejuni. Infect Immun. 1990 Jul;58(7):2214–2219. doi: 10.1128/iai.58.7.2214-2219.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blaser M. J., Black R. E., Duncan D. J., Amer J. Campylobacter jejuni-specific serum antibodies are elevated in healthy Bangladeshi children. J Clin Microbiol. 1985 Feb;21(2):164–167. doi: 10.1128/jcm.21.2.164-167.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Blaser M. J., Duncan D. J. Human serum antibody response to Campylobacter jejuni infection as measured in an enzyme-linked immunosorbent assay. Infect Immun. 1984 May;44(2):292–298. doi: 10.1128/iai.44.2.292-298.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Burr D. H., Caldwell M. B., Bourgeois A. L., Morgan H. R., Wistar R., Jr, Walker R. I. Mucosal and systemic immunity to Campylobacter jejuni in rabbits after gastric inoculation. Infect Immun. 1988 Jan;56(1):99–105. doi: 10.1128/iai.56.1.99-105.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Burr D. H., Kerner D. T., Blanco C. S., Bourgeois A. L., Wistar R., Jr Gastric lavage: a simple method to obtain IgA-rich intestinal secretions from the rabbit. J Immunol Methods. 1987 May 20;99(2):277–281. doi: 10.1016/0022-1759(87)90138-4. [DOI] [PubMed] [Google Scholar]
  7. Fischer S. H., Nachamkin I. Common and variable domains of the flagellin gene, flaA, in Campylobacter jejuni. Mol Microbiol. 1991 May;5(5):1151–1158. doi: 10.1111/j.1365-2958.1991.tb01888.x. [DOI] [PubMed] [Google Scholar]
  8. Guerry P., Logan S. M., Thornton S., Trust T. J. Genomic organization and expression of Campylobacter flagellin genes. J Bacteriol. 1990 Apr;172(4):1853–1860. doi: 10.1128/jb.172.4.1853-1860.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lane E. M., Batchelor R. A., Bourgeois A. L., Burr D. H., Olson J. G. Urine and faecal IgA response during naturally acquired infection with Campylobacter jejuni. Lancet. 1987 May 16;1(8542):1141–1141. doi: 10.1016/s0140-6736(87)91694-1. [DOI] [PubMed] [Google Scholar]
  10. Lee A., O'Rourke J. L., Barrington P. J., Trust T. J. Mucus colonization as a determinant of pathogenicity in intestinal infection by Campylobacter jejuni: a mouse cecal model. Infect Immun. 1986 Feb;51(2):536–546. doi: 10.1128/iai.51.2.536-546.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lior H., Woodward D. L., Edgar J. A., Laroche L. J., Gill P. Serotyping of Campylobacter jejuni by slide agglutination based on heat-labile antigenic factors. J Clin Microbiol. 1982 May;15(5):761–768. doi: 10.1128/jcm.15.5.761-768.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Logan S. M., Trust T. J., Guerry P. Evidence for posttranslational modification and gene duplication of Campylobacter flagellin. J Bacteriol. 1989 Jun;171(6):3031–3038. doi: 10.1128/jb.171.6.3031-3038.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Martin P. M., Mathiot J., Ipero J., Kirimat M., Georges A. J., Georges-Courbot M. C. Immune response to Campylobacter jejuni and Campylobacter coli in a cohort of children from birth to 2 years of age. Infect Immun. 1989 Aug;57(8):2542–2546. doi: 10.1128/iai.57.8.2542-2546.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Mascart-Lemone F. O., Duchateau J. R., Oosterom J., Butzler J. P., Delacroix D. L. Kinetics of anti-Campylobacter jejuni monomeric and polymeric immunoglobulin A1 and A2 responses in serum during acute enteritis. J Clin Microbiol. 1987 Jul;25(7):1253–1257. doi: 10.1128/jcm.25.7.1253-1257.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. McSweegan E., Burr D. H., Walker R. I. Intestinal mucus gel and secretory antibody are barriers to Campylobacter jejuni adherence to INT 407 cells. Infect Immun. 1987 Jun;55(6):1431–1435. doi: 10.1128/iai.55.6.1431-1435.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Morooka T., Umeda A., Amako K. Motility as an intestinal colonization factor for Campylobacter jejuni. J Gen Microbiol. 1985 Aug;131(8):1973–1980. doi: 10.1099/00221287-131-8-1973. [DOI] [PubMed] [Google Scholar]
  17. Nachamkin I., Hart A. M. Common and specific epitopes of Campylobacter flagellin recognized by monoclonal antibodies. Infect Immun. 1986 Aug;53(2):438–440. doi: 10.1128/iai.53.2.438-440.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Nachamkin I., Yang X. H. Human antibody response to Campylobacter jejuni flagellin protein and a synthetic N-terminal flagellin peptide. J Clin Microbiol. 1989 Oct;27(10):2195–2198. doi: 10.1128/jcm.27.10.2195-2198.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Nuijten P. J., van Asten F. J., Gaastra W., van der Zeijst B. A. Structural and functional analysis of two Campylobacter jejuni flagellin genes. J Biol Chem. 1990 Oct 15;265(29):17798–17804. [PubMed] [Google Scholar]
  20. Nuijten P. J., van der Zeijst B. A., Newell D. G. Localization of immunogenic regions on the flagellin proteins of Campylobacter jejuni 81116. Infect Immun. 1991 Mar;59(3):1100–1105. doi: 10.1128/iai.59.3.1100-1105.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Pavlovskis O. R., Rollins D. M., Haberberger R. L., Jr, Green A. E., Habash L., Strocko S., Walker R. I. Significance of flagella in colonization resistance of rabbits immunized with Campylobacter spp. Infect Immun. 1991 Jul;59(7):2259–2264. doi: 10.1128/iai.59.7.2259-2264.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Winsor D. K., Jr, Mathewson J. J., DuPont H. L. Western blot analysis of intestinal secretory immunoglobulin A response to Campylobacter jejuni antigens in patients with naturally acquired Campylobacter enteritis. Gastroenterology. 1986 May;90(5 Pt 1):1217–1222. doi: 10.1016/0016-5085(86)90388-4. [DOI] [PubMed] [Google Scholar]

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