Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Mar;30(3):595–599. doi: 10.1128/jcm.30.3.595-599.1992

Isolation of a mucoid alginate-producing Pseudomonas aeruginosa strain from the equine guttural pouch.

J R Govan 1, P Sarasola 1, D J Taylor 1, P J Tatnell 1, N J Russell 1, P Gacesa 1
PMCID: PMC265116  PMID: 1551975

Abstract

The isolation and characterization of a mucoid, alginate-producing strain of Pseudomonas aeruginosa from a nonhuman host, namely, in chondroids from an equine guttural pouch, is reported for the first time. Pure cultures of P. aeruginosa 12534 were isolated from a 17-month-old pony mare with a history of chronic bilateral mucopurulent nasal discharge from the right guttural pouch. Transmission electron microscopy of chondroids showed mucoid P. aeruginosa growing as microcolonies within a matrix of extracellular material. On the basis of expression of the mucoid phenotype under different growth conditions, P. aeruginosa 12534 belongs to group 1 and resembles other isolates carrying the muc-23 mutation. The bulk of the extracellular material was characterized as being alginate by chemical and 1H nuclear magnetic resonance analyses, which showed that it had a composition similar to that produced by isolates of P. aeruginosa from human patients with cystic fibrosis.

Full text

PDF
595

Images in this article

597Image
on p.597
597Image
on p.597
598Image
on p.598

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Anastassiou E. D., Mintzas A. C., Kounavis C., Dimitracopoulos G. Alginate production by clinical nonmucoid Pseudomonas aeruginosa strains. J Clin Microbiol. 1987 Apr;25(4):656–659. doi: 10.1128/jcm.25.4.656-659.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. BULLOCK G. L. CHARACTERISTICS AND PATHOGENICITY OF A CAPSULATED PSEUDOMONAS ISOLATED FROM GOLDFISH. Appl Microbiol. 1965 Jan;13:89–92. doi: 10.1128/am.13.1.89-92.1965. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Blumenkrantz N., Asboe-Hansen G. New method for quantitative determination of uronic acids. Anal Biochem. 1973 Aug;54(2):484–489. doi: 10.1016/0003-2697(73)90377-1. [DOI] [PubMed] [Google Scholar]
  4. Bradford M. M. A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding. Anal Biochem. 1976 May 7;72:248–254. doi: 10.1016/0003-2697(76)90527-3. [DOI] [PubMed] [Google Scholar]
  5. Buscher H. P., Casals-Stenzel J., Schaufer R. New sialic acids. Identification of N-glycoloyl-O-acetylneuraminic acids and N-acetyl-O-glycoloylneuraminic acids by improved methods for detection of N-acyl and O-acyl groups and by gas-liquid chromatography. Eur J Biochem. 1974 Dec 16;50(1):71–82. doi: 10.1111/j.1432-1033.1974.tb03873.x. [DOI] [PubMed] [Google Scholar]
  6. Deretic V., Govan J. R., Konyecsni W. M., Martin D. W. Mucoid Pseudomonas aeruginosa in cystic fibrosis: mutations in the muc loci affect transcription of the algR and algD genes in response to environmental stimuli. Mol Microbiol. 1990 Feb;4(2):189–196. doi: 10.1111/j.1365-2958.1990.tb00586.x. [DOI] [PubMed] [Google Scholar]
  7. Fett W. F., Osman S. F., Dunn M. F. Characterization of exopolysaccharides produced by plant-associated fluorescent pseudomonads. Appl Environ Microbiol. 1989 Mar;55(3):579–583. doi: 10.1128/aem.55.3.579-583.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Govan J. R., Fyfe J. A., Jarman T. R. Isolation of alginate-producing mutants of Pseudomonas fluorescens, Pseudomonas putida and Pseudomonas mendocina. J Gen Microbiol. 1981 Jul;125(1):217–220. doi: 10.1099/00221287-125-1-217. [DOI] [PubMed] [Google Scholar]
  9. HUGH R., LEIFSON E. The taxonomic significance of fermentative versus oxidative metabolism of carbohydrates by various gram negative bacteria. J Bacteriol. 1953 Jul;66(1):24–26. doi: 10.1128/jb.66.1.24-26.1953. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. KOVACS N. Identification of Pseudomonas pyocyanea by the oxidase reaction. Nature. 1956 Sep 29;178(4535):703–703. doi: 10.1038/178703a0. [DOI] [PubMed] [Google Scholar]
  11. Lam J., Chan R., Lam K., Costerton J. W. Production of mucoid microcolonies by Pseudomonas aeruginosa within infected lungs in cystic fibrosis. Infect Immun. 1980 May;28(2):546–556. doi: 10.1128/iai.28.2.546-556.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. May T. B., Shinabarger D., Maharaj R., Kato J., Chu L., DeVault J. D., Roychoudhury S., Zielinski N. A., Berry A., Rothmel R. K. Alginate synthesis by Pseudomonas aeruginosa: a key pathogenic factor in chronic pulmonary infections of cystic fibrosis patients. Clin Microbiol Rev. 1991 Apr;4(2):191–206. doi: 10.1128/cmr.4.2.191. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. McAvoy M. J., Newton V., Paull A., Morgan J., Gacesa P., Russell N. J. Isolation of mucoid strains of Pseudomonas aeruginosa from non-cystic-fibrosis patients and characterisation of the structure of their secreted alginate. J Med Microbiol. 1989 Mar;28(3):183–189. doi: 10.1099/00222615-28-3-183. [DOI] [PubMed] [Google Scholar]
  14. Narbad A., Russell N. J., Gacesa P. Radiolabelling patterns in alginate of Pseudomonas aeruginosa synthesized from specifically-labelled 14C-monosaccharide precursors. Microbios. 1988;54(220-221):171–179. [PubMed] [Google Scholar]
  15. Osman S. F., Fett W. F., Fishman M. L. Exopolysaccharides of the phytopathogen Pseudomonas syringae pv. glycinea. J Bacteriol. 1986 Apr;166(1):66–71. doi: 10.1128/jb.166.1.66-71.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Phillips I. Identification of Pseudomonas aeruginosa in the clinical laboratory. J Med Microbiol. 1969 Feb;2(1):9–16. doi: 10.1099/00222615-2-1-9. [DOI] [PubMed] [Google Scholar]
  17. Russell N. J., Gacesa P. Chemistry and biology of the alginate of mucoid strains of Pseudomonas aeruginosa in cystic fibrosis. Mol Aspects Med. 1988;10(1):1–91. doi: 10.1016/0098-2997(88)90002-7. [DOI] [PubMed] [Google Scholar]
  18. Sherbrock-Cox V., Russell N. J., Gacesa P. The purification and chemical characterisation of the alginate present in extracellular material produced by mucoid strains of Pseudomonas aeruginosa. Carbohydr Res. 1984 Dec 15;135(1):147–154. doi: 10.1016/0008-6215(84)85012-0. [DOI] [PubMed] [Google Scholar]
  19. Skjåk-Braek G., Grasdalen H., Larsen B. Monomer sequence and acetylation pattern in some bacterial alginates. Carbohydr Res. 1986 Oct 15;154:239–250. doi: 10.1016/s0008-6215(00)90036-3. [DOI] [PubMed] [Google Scholar]
  20. Springer E. L., Roth I. L. The ultrastructure of the capsules of Diplococcus pneumoniae and Klebsiella pneumoniae stained with ruthenium red. J Gen Microbiol. 1973 Jan;74(1):21–31. doi: 10.1099/00221287-74-1-21. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES