Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Mar;30(3):704–711. doi: 10.1128/jcm.30.3.704-711.1992

Comparisons of rotavirus VP7-typing monoclonal antibodies by competition binding assay.

P Raj 1, D O Matson 1, B S Coulson 1, R F Bishop 1, K Taniguchi 1, S Urasawa 1, H B Greenberg 1, M K Estes 1
PMCID: PMC265136  PMID: 1372622

Abstract

Three sets of neutralizing monoclonal antibodies (MAbs) used to type the outer capsid protein VP7 of four group A rotavirus serotypes (1 through 4) were compared in competition immunoassays. Reciprocal competition was observed for each of the VP7 type 2-, 3-, and 4-specific MAbs. The VP7 type 1 MAbs exhibited variable competition patterns with other VP7 type 1 MAbs. MAb RV4:3, which has been used to recognize antigenic variants within VP7 type 1 strains, showed reciprocal competition with the four VP7 type 3 MAbs (RV3:1, YO-1E2, 4F8, and 159) using a VP7 type 3 virus (SA11) as antigen. MAb 2C9, also prepared against VP7 type 1, reacted with VP7 type 3 strains and competed with a VP7 type 3 MAb, 159, using RRV as antigen. Use of the different sets of VP7 type-specific MAbs in the enzyme-linked immunosorbent assay permitted the recognition of six antigenic variants within VP7 types 1, 2, and 3 among specimens whose VP7 type could not be determined previously with only one set of typing MAbs. These results demonstrate differences of typing ability among these VP7-specific MAbs and emphasize the need to improve the sensitivity of typing systems by incorporating panels of MAbs reacting with several neutralizing epitopes.

Full text

PDF
704

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Burns J. W., Greenberg H. B., Shaw R. D., Estes M. K. Functional and topographical analyses of epitopes on the hemagglutinin (VP4) of the simian rotavirus SA11. J Virol. 1988 Jun;62(6):2164–2172. doi: 10.1128/jvi.62.6.2164-2172.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Coulson B. S., Kirkwood C. Relation of VP7 amino acid sequence to monoclonal antibody neutralization of rotavirus and rotavirus monotype. J Virol. 1991 Nov;65(11):5968–5974. doi: 10.1128/jvi.65.11.5968-5974.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Coulson B. S., Tursi J. M., McAdam W. J., Bishop R. F. Derivation of neutralizing monoclonal antibodies to human rotaviruses and evidence that an immunodominant neutralization site is shared between serotypes 1 and 3. Virology. 1986 Oct 30;154(2):302–312. doi: 10.1016/0042-6822(86)90456-3. [DOI] [PubMed] [Google Scholar]
  4. Coulson B. S., Unicomb L. E., Pitson G. A., Bishop R. F. Simple and specific enzyme immunoassay using monoclonal antibodies for serotyping human rotaviruses. J Clin Microbiol. 1987 Mar;25(3):509–515. doi: 10.1128/jcm.25.3.509-515.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coulson B. S. Variation in neutralization epitopes of human rotaviruses in relation to genomic RNA polymorphism. Virology. 1987 Aug;159(2):209–216. doi: 10.1016/0042-6822(87)90457-0. [DOI] [PubMed] [Google Scholar]
  6. Dimitrov D. H., Graham D. Y., Lopez J., Muchinik G., Velasco G., Stenback W. A., Estes M. K. RNA electropherotypes of human rotaviruses from North and South America. Bull World Health Organ. 1984;62(2):321–329. [PMC free article] [PubMed] [Google Scholar]
  7. Dyall-Smith M. L., Lazdins I., Tregear G. W., Holmes I. H. Location of the major antigenic sites involved in rotavirus serotype-specific neutralization. Proc Natl Acad Sci U S A. 1986 May;83(10):3465–3468. doi: 10.1073/pnas.83.10.3465. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Estes M. K., Cohen J. Rotavirus gene structure and function. Microbiol Rev. 1989 Dec;53(4):410–449. doi: 10.1128/mr.53.4.410-449.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Estes M. K., Graham D. Y., Smith E. M., Gerba C. P. Rotavirus stability and inactivation. J Gen Virol. 1979 May;43(2):403–409. doi: 10.1099/0022-1317-43-2-403. [DOI] [PubMed] [Google Scholar]
  10. Estes M. K., Palmer E. L., Obijeski J. F. Rotaviruses: a review. Curr Top Microbiol Immunol. 1983;105:123–184. doi: 10.1007/978-3-642-69159-1_3. [DOI] [PubMed] [Google Scholar]
  11. Gerna G., Sarasini A., di Matteo A., Parea M., Orsolini P., Battaglia M. Identification of two subtypes of serotype 4 human rotavirus by using VP7-specific neutralizing monoclonal antibodies. J Clin Microbiol. 1988 Jul;26(7):1388–1392. doi: 10.1128/jcm.26.7.1388-1392.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Gouvea V., Ho M. S., Glass R., Woods P., Forrester B., Robinson C., Ashley R., Riepenhoff-Talty M., Clark H. F., Taniguchi K. Serotypes and electropherotypes of human rotavirus in the USA: 1987-1989. J Infect Dis. 1990 Aug;162(2):362–367. doi: 10.1093/infdis/162.2.362. [DOI] [PubMed] [Google Scholar]
  13. Green K. Y., James H. D., Jr, Kapikian A. Z. Evaluation of three panels of monoclonal antibodies for the identification of human rotavirus VP7 serotype by ELISA. Bull World Health Organ. 1990;68(5):601–610. [PMC free article] [PubMed] [Google Scholar]
  14. Green K. Y., Midthun K., Gorziglia M., Hoshino Y., Kapikian A. Z., Chanock R. M., Flores J. Comparison of the amino acid sequences of the major neutralization protein of four human rotavirus serotypes. Virology. 1987 Nov;161(1):153–159. doi: 10.1016/0042-6822(87)90181-4. [DOI] [PubMed] [Google Scholar]
  15. Green K. Y., Sears J. F., Taniguchi K., Midthun K., Hoshino Y., Gorziglia M., Nishikawa K., Urasawa S., Kapikian A. Z., Chanock R. M. Prediction of human rotavirus serotype by nucleotide sequence analysis of the VP7 protein gene. J Virol. 1988 May;62(5):1819–1823. doi: 10.1128/jvi.62.5.1819-1823.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Green K. Y., Taniguchi K., Mackow E. R., Kapikian A. Z. Homotypic and heterotypic epitope-specific antibody responses in adult and infant rotavirus vaccinees: implications for vaccine development. J Infect Dis. 1990 Apr;161(4):667–679. doi: 10.1093/infdis/161.4.667. [DOI] [PubMed] [Google Scholar]
  17. Greenberg H. B., Flores J., Kalica A. R., Wyatt R. G., Jones R. Gene coding assignments for growth restriction, neutralization and subgroup specificities of the W and DS-1 strains of human rotavirus. J Gen Virol. 1983 Feb;64(Pt 2):313–320. doi: 10.1099/0022-1317-64-2-313. [DOI] [PubMed] [Google Scholar]
  18. Heath R., Birch C., Gust I. Antigenic analysis of rotavirus isolates using monoclonal antibodies specific for human serotypes 1, 2, 3 and 4, and SA11. J Gen Virol. 1986 Nov;67(Pt 11):2455–2466. doi: 10.1099/0022-1317-67-11-2455. [DOI] [PubMed] [Google Scholar]
  19. Hoshino Y., Sereno M. M., Midthun K., Flores J., Kapikian A. Z., Chanock R. M. Independent segregation of two antigenic specificities (VP3 and VP7) involved in neutralization of rotavirus infectivity. Proc Natl Acad Sci U S A. 1985 Dec;82(24):8701–8704. doi: 10.1073/pnas.82.24.8701. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Kitamoto N., Ramig R. F., Matson D. O., Estes M. K. Comparative growth of different rotavirus strains in differentiated cells (MA104, HepG2, and CaCo-2). Virology. 1991 Oct;184(2):729–737. doi: 10.1016/0042-6822(91)90443-f. [DOI] [PubMed] [Google Scholar]
  21. Kobayashi N., Taniguchi K., Urasawa S. Analysis of the newly identified neutralization epitopes on VP7 of human rotavirus serotype 1. J Gen Virol. 1991 Jan;72(Pt 1):117–124. doi: 10.1099/0022-1317-72-1-117. [DOI] [PubMed] [Google Scholar]
  22. Mackow E. R., Shaw R. D., Matsui S. M., Vo P. T., Benfield D. A., Greenberg H. B. Characterization of homotypic and heterotypic VP7 neutralization sites of rhesus rotavirus. Virology. 1988 Aug;165(2):511–517. doi: 10.1016/0042-6822(88)90595-8. [DOI] [PubMed] [Google Scholar]
  23. Matson D. O., Estes M. K., Burns J. W., Greenberg H. B., Taniguchi K., Urasawa S. Serotype variation of human group A rotaviruses in two regions of the USA. J Infect Dis. 1990 Sep;162(3):605–614. doi: 10.1093/infdis/162.3.605. [DOI] [PubMed] [Google Scholar]
  24. Nishikawa K., Hoshino Y., Taniguchi K., Green K. Y., Greenberg H. B., Kapikian A. Z., Chanock R. M., Gorziglia M. Rotavirus VP7 neutralization epitopes of serotype 3 strains. Virology. 1989 Aug;171(2):503–515. doi: 10.1016/0042-6822(89)90620-x. [DOI] [PubMed] [Google Scholar]
  25. Offit P. A., Blavat G. Identification of the two rotavirus genes determining neutralization specificities. J Virol. 1986 Jan;57(1):376–378. doi: 10.1128/jvi.57.1.376-378.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Offit P. A., Clark H. F., Blavat G., Greenberg H. B. Reassortant rotaviruses containing structural proteins vp3 and vp7 from different parents induce antibodies protective against each parental serotype. J Virol. 1986 Nov;60(2):491–496. doi: 10.1128/jvi.60.2.491-496.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  27. Offit P. A., Shaw R. D., Greenberg H. B. Passive protection against rotavirus-induced diarrhea by monoclonal antibodies to surface proteins vp3 and vp7. J Virol. 1986 May;58(2):700–703. doi: 10.1128/jvi.58.2.700-703.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Padilla-Noriega L., Arias C. F., López S., Puerto F., Snodgrass D. R., Taniguchi K., Greenberg H. B. Diversity of rotavirus serotypes in Mexican infants with gastroenteritis. J Clin Microbiol. 1990 Jun;28(6):1114–1119. doi: 10.1128/jcm.28.6.1114-1119.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Shaw R. D., Fong K. J., Losonsky G. A., Levine M. M., Maldonado Y., Yolken R., Flores J., Kapikian A. Z., Vo P. T., Greenberg H. B. Epitope-specific immune responses to rotavirus vaccination. Gastroenterology. 1987 Nov;93(5):941–950. doi: 10.1016/0016-5085(87)90555-5. [DOI] [PubMed] [Google Scholar]
  30. Shaw R. D., Stoner-Ma D. L., Estes M. K., Greenberg H. B. Specific enzyme-linked immunoassay for rotavirus serotypes 1 and 3. J Clin Microbiol. 1985 Aug;22(2):286–291. doi: 10.1128/jcm.22.2.286-291.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Shaw R. D., Vo P. T., Offit P. A., Coulson B. S., Greenberg H. B. Antigenic mapping of the surface proteins of rhesus rotavirus. Virology. 1986 Dec;155(2):434–451. doi: 10.1016/0042-6822(86)90205-9. [DOI] [PubMed] [Google Scholar]
  32. Taniguchi K., Hoshino Y., Nishikawa K., Green K. Y., Maloy W. L., Morita Y., Urasawa S., Kapikian A. Z., Chanock R. M., Gorziglia M. Cross-reactive and serotype-specific neutralization epitopes on VP7 of human rotavirus: nucleotide sequence analysis of antigenic mutants selected with monoclonal antibodies. J Virol. 1988 Jun;62(6):1870–1874. doi: 10.1128/jvi.62.6.1870-1874.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  33. Taniguchi K., Urasawa T., Morita Y., Greenberg H. B., Urasawa S. Direct serotyping of human rotavirus in stools by an enzyme-linked immunosorbent assay using serotype 1-, 2-, 3-, and 4-specific monoclonal antibodies to VP7. J Infect Dis. 1987 Jun;155(6):1159–1166. doi: 10.1093/infdis/155.6.1159. [DOI] [PubMed] [Google Scholar]
  34. Unicomb L. E., Bishop R. F. Epidemiology of rotavirus strains infecting children throughout Australia during 1986-1987. A study of serotype and RNA electropherotype. Arch Virol. 1989;106(1-2):23–34. doi: 10.1007/BF01311035. [DOI] [PubMed] [Google Scholar]
  35. Urasawa S., Urasawa T., Taniguchi K., Wakasugi F., Kobayashi N., Chiba S., Sakurada N., Morita M., Morita O., Tokieda M. Survey of human rotavirus serotypes in different locales in Japan by enzyme-linked immunosorbent assay with monoclonal antibodies. J Infect Dis. 1989 Jul;160(1):44–51. doi: 10.1093/infdis/160.1.44. [DOI] [PubMed] [Google Scholar]
  36. Ward R. L., McNeal M. M., Clemens J. D., Sack D. A., Rao M., Huda N., Green K. Y., Kapikian A. Z., Coulson B. S., Bishop R. F. Reactivities of serotyping monoclonal antibodies with culture-adapted human rotaviruses. J Clin Microbiol. 1991 Mar;29(3):449–456. doi: 10.1128/jcm.29.3.449-456.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES