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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Mar;30(3):726–728. doi: 10.1128/jcm.30.3.726-728.1992

Evaluation of the Genetic Systems Corp. Integra HIV-1 Pageblot system.

C A Starkey 1, B Yen-Lieberman 1, M R Proffitt 1
PMCID: PMC265142  PMID: 1551992

Abstract

The Genetic Systems Corp. Integra HIV-1 Pageblot system was evaluated as a supplementary assay to confirm the presence of antibodies to human immunodeficiency virus type 1 in 57 specimens from individuals at high risk of infection with the virus. Forty-one specimens identified as reactive in the Genetic Systems Integra HIV-1 Pageblot system were likewise identified as reactive in a U.S. Food and Drug Administration-licensed (Biotech/Dupont) Western blot (immunoblot). Six specimens identified as indeterminate in either or both immunoblot assays were all identified as nonreactive in a U.S. Food and Drug Administration-licensed enzyme immunoassay with recombinant antigens.

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Selected References

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  1. Biberfeld G., Bredberg-Rådén U., Böttiger B., Putkonen P. O., Blomberg J., Juto P., Wadell G. Blood donor sera with false-positive Western blot reactions to human immunodeficiency virus. Lancet. 1986 Aug 2;2(8501):289–290. doi: 10.1016/s0140-6736(86)92111-2. [DOI] [PubMed] [Google Scholar]
  2. Davenport A., Grant P. J. False-positive autoantibodies in HIV infection. Lancet. 1990 Aug 4;336(8710):317–318. doi: 10.1016/0140-6736(90)91857-7. [DOI] [PubMed] [Google Scholar]
  3. Fortes P., Menitove J., Ross A., Steece R., Cabrian K., Ferrera C., Perkins P. A., Sturge J., Lealos R., Krieger M. S. Evaluation of blood collected on filter paper for detection of antibodies to human immunodeficiency virus type 1. J Clin Microbiol. 1989 Jun;27(6):1380–1381. doi: 10.1128/jcm.27.6.1380-1381.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Genesca J., Shih J. W., Jett B. W., Hewlett I. K., Epstein J. S., Alter H. J. What do western blot indeterminate patterns for human immunodeficiency virus mean in EIA-negative blood donors? Lancet. 1989 Oct 28;2(8670):1023–1025. doi: 10.1016/s0140-6736(89)91027-1. [DOI] [PubMed] [Google Scholar]
  5. Gonzalez-Scarano F., Waxham M. N., Ross A. M., Hoxie J. A. Sequence similarities between human immunodeficiency virus gp41 and paramyxovirus fusion proteins. AIDS Res Hum Retroviruses. 1987 Fall;3(3):245–252. doi: 10.1089/aid.1987.3.245. [DOI] [PubMed] [Google Scholar]
  6. Henderson L. E., Sowder R., Copeland T. D., Oroszlan S., Arthur L. O., Robey W. G., Fischinger P. J. Direct identification of class II histocompatibility DR proteins in preparations of human T-cell lymphotropic virus type III. J Virol. 1987 Feb;61(2):629–632. doi: 10.1128/jvi.61.2.629-632.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Heyward W. L., Curran J. W. Rapid screening tests for HIV infection. JAMA. 1988 Jul 22;260(4):542–542. doi: 10.1001/jama.260.4.542. [DOI] [PubMed] [Google Scholar]
  8. Hoff R., Berardi V. P., Weiblen B. J., Mahoney-Trout L., Mitchell M. L., Grady G. F. Seroprevalence of human immunodeficiency virus among childbearing women. Estimation by testing samples of blood from newborns. N Engl J Med. 1988 Mar 3;318(9):525–530. doi: 10.1056/NEJM198803033180901. [DOI] [PubMed] [Google Scholar]
  9. Midthun K., Garrison L., Clements M. L., Farzadegan H., Fernie B., Quinn T. Frequency of indeterminate western blot tests in healthy adults at low risk for human immunodeficiency virus infection. The NIAID AIDS Vaccine Clinical Trails Network. J Infect Dis. 1990 Dec;162(6):1379–1382. doi: 10.1093/infdis/162.6.1379. [DOI] [PubMed] [Google Scholar]
  10. Povolotsky J., Gold J. W., Chein N., Baron P., Armstrong D. Differences in human immunodeficiency virus type 1 (HIV-1) anti-p24 reactivities in serum of HIV-1-infected and uninfected subjects: analysis of indeterminate western blot reactions. J Infect Dis. 1991 Feb;163(2):247–251. doi: 10.1093/infdis/163.2.247. [DOI] [PubMed] [Google Scholar]
  11. Sayers M. H., Beatty P. G., Hansen J. A. HLA antibodies as a cause of false-positive reactions in screening enzyme immunoassays for antibodies to human T-lymphotropic virus type III. Transfusion. 1986 Jan-Feb;26(1):113–115. doi: 10.1046/j.1537-2995.1986.26186124012.x. [DOI] [PubMed] [Google Scholar]
  12. Schuurman H. J., Krone W. J., Broekhuizen R., van Baarlen J., van Veen P., Golstein A. L., Huber J., Goudsmit J. The thymus in acquired immune deficiency syndrome. Comparison with other types of immunodeficiency diseases, and presence of components of human immunodeficiency virus type 1. Am J Pathol. 1989 Jun;134(6):1329–1338. [PMC free article] [PubMed] [Google Scholar]
  13. Schwartz J. S., Dans P. E., Kinosian B. P. Human immunodeficiency virus test evaluation, performance, and use. Proposals to make good tests better. JAMA. 1988 May 6;259(17):2574–2579. [PubMed] [Google Scholar]
  14. Wittwer C. T., Smith A. M., Ash K. O., DeWitt C. W. False-positive antibody tests for human immunodeficiency virus in transplant patients with antilymphocyte antibodies. Transplantation. 1987 Dec;44(6):843–844. [PubMed] [Google Scholar]
  15. Yu S. K., Fong C. K., Landry M. L., Hsiung G. D., Solomon L. R. A false positive HIV antibody reaction due to transfusion-induced HLA-DR4 sensitization. N Engl J Med. 1989 Jun 1;320(22):1495–1496. doi: 10.1056/NEJM198906013202215. [DOI] [PubMed] [Google Scholar]
  16. van der Poel C. L., Lelie P. N., Reesink H. W., van Exel-Oehlers P. J., Tersmette M., van den Akker R., Gonzalves M., Huisman J. G. Blood donors with indeterminate anti-p24gag reactivity in HIV-1 western blot: absence of infectivity to transfused patients and in virus culture. Vox Sang. 1989;56(3):162–167. doi: 10.1111/j.1423-0410.1989.tb02020.x. [DOI] [PubMed] [Google Scholar]

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