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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Mar;30(3):732–734. doi: 10.1128/jcm.30.3.732-734.1992

Development of an immunoassay using recombinant maltose-binding protein-STa fusions for quantitating antibody responses against STa, the heat-stable enterotoxin of Escherichia coli.

R Aitken 1, T R Hirst 1
PMCID: PMC265144  PMID: 1551993

Abstract

A set of fusion proteins containing heat-stable enterotoxin (STa) and maltose-binding protein were engineered. These molecules were readily purified and used as solid-phase antigens in an enzyme-linked immunosorbent assay to monitor anti-STa responses in mice immunized with a recombinant vaccine composed of STa and the B subunit of heat-labile enterotoxin.

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Selected References

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