Abstract
Twenty-one type or other reference strains, each representing a different Campylobacter, Helicobacter, or Arcobacter taxon, and a reference strain of Staphylococcus aureus were used to assess the reproducibility of nine enzyme detection tests used in the identification of campylobacters. For five of the tests (alkaline phosphatase, DNase, and H2S production, indoxyl acetate hydrolysis, and nitrate reduction), more than one procedure was employed to determine the most suitable method. Alkaline phosphatase test results were better defined and more reproducible if read after 1 h of incubation. Detection of DNase was fully reproducible with each method (except with Helicobacter pylori), but reactions were generally weaker than those of other DNase-producing organisms. Both procedures for determining H2S production were irreproducible for the same strains. The reproducibility of indoxyl acetate hydrolysis was improved by using disks impregnated with 25 microliters of substrate. Reduction of nitrate was best determined by Cook's plate method. Results for the other tests examined (catalase, oxidase, and urease production and hippurate hydrolysis) were both pertinent and fully reproducible for all strains.
Full text
PDF



Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bascomb S., Lapage S. P., Curtis M. A., Willcox W. R. Identification of bacteria by computer: identification of reference strains. J Gen Microbiol. 1973 Aug;77(2):291–315. doi: 10.1099/00221287-77-2-291. [DOI] [PubMed] [Google Scholar]
- Cook G. T. A Plate Test for Nitrate Reduction. J Clin Pathol. 1950 Nov;3(4):359–362. doi: 10.1136/jcp.3.4.359. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Holmes B., Dawson C. A., Pinning C. A. A revised probability matrix for the identification of gram-negative, aerobic, rod-shaped, fermentative bacteria. J Gen Microbiol. 1986 Nov;132(11):3113–3135. doi: 10.1099/00221287-132-11-3113. [DOI] [PubMed] [Google Scholar]
- Hänninen M. L. Rapid method for the detection of DNase of campylobacters. J Clin Microbiol. 1989 Sep;27(9):2118–2119. doi: 10.1128/jcm.27.9.2118-2119.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Itoh T., Yanagawa Y., Shingaki M., Takahashi M., Kai A., Ohashi M., Hamana G. Isolation of Campylobacter pyloridis from human gastric mucosa and characterization of the isolates. Microbiol Immunol. 1987;31(7):603–614. doi: 10.1111/j.1348-0421.1987.tb03121.x. [DOI] [PubMed] [Google Scholar]
- Lior H., Patel A. Improved toluidine blue-DNA agar for detection of DNA hydrolysis by campylobacters. J Clin Microbiol. 1987 Oct;25(10):2030–2031. doi: 10.1128/jcm.25.10.2030-2031.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Mills C. K., Gherna R. L. Hydrolysis of indoxyl acetate by Campylobacter species. J Clin Microbiol. 1987 Aug;25(8):1560–1561. doi: 10.1128/jcm.25.8.1560-1561.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- On S. L., Holmes B. Effect of inoculum size on the phenotypic characterization of Campylobacter species. J Clin Microbiol. 1991 May;29(5):923–926. doi: 10.1128/jcm.29.5.923-926.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- On S. L., Holmes B. Reproducibility of tolerance tests that are useful in the identification of campylobacteria. J Clin Microbiol. 1991 Sep;29(9):1785–1788. doi: 10.1128/jcm.29.9.1785-1788.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Owen R. J., Martin S. R., Borman P. Rapid urea hydrolysis by gastric Campylobacters. Lancet. 1985 Jan 12;1(8420):111–111. doi: 10.1016/s0140-6736(85)92004-5. [DOI] [PubMed] [Google Scholar]
- Roop R. M., 2nd, Smibert R. M., Johnson J. L., Krieg N. R. Differential characteristics of catalase-positive campylobacters correlated with DNA homology groups. Can J Microbiol. 1984 Jul;30(7):938–951. doi: 10.1139/m84-147. [DOI] [PubMed] [Google Scholar]
- Skirrow M. B., Benjamin J. Differentiation of enteropathogenic Campylobacter. J Clin Pathol. 1980 Nov;33(11):1122–1122. doi: 10.1136/jcp.33.11.1122. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Vandamme P., Falsen E., Rossau R., Hoste B., Segers P., Tytgat R., De Ley J. Revision of Campylobacter, Helicobacter, and Wolinella taxonomy: emendation of generic descriptions and proposal of Arcobacter gen. nov. Int J Syst Bacteriol. 1991 Jan;41(1):88–103. doi: 10.1099/00207713-41-1-88. [DOI] [PubMed] [Google Scholar]