Abstract
Growth value thresholds used to identify positive blood culture vials can be defined by users for each BACTEC NR-660 bacteremia detection instrument. Growth values were compared with the recovery of organisms from vials flagged as positive during the testing of 3.056 high-volume vials containing aerobic (BACTEC PLUS 26) medium over a 2-month period. Results showed that optimal threshold values for our use of these vials varied from those recommended by the manufacturer; if the thresholds defined from these data had been used during the study period, total vials flagged as positive from which no organisms were recovered (false alarms) would have been reduced from 181 (5.9/100 vials tested) to 71 (2.3/100 vials tested), with a minimal decrease in the identification of vials containing usual or occasional pathogens (hits). Adjustments of growth value thresholds by the individual user can make the use of BACTEC instruments more efficient by decreasing further processing of vials from which no organisms are recovered.
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Selected References
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- Anderson J. D., Trombley C., Cimolai N. Assessment of the BACTEC NR660 blood culture system for the detection of bacteremia in young children. J Clin Microbiol. 1989 Apr;27(4):721–723. doi: 10.1128/jcm.27.4.721-723.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Courcol R. J., Durocher A. V., Robin H., Martin G. R. Influence of blood carbon dioxide pressure on growth index values in non-radiometric Bactec vials. Eur J Clin Microbiol Infect Dis. 1989 May;8(5):400–401. doi: 10.1007/BF01964055. [DOI] [PubMed] [Google Scholar]
- Courcol R. J., Fruchart A., Roussel-Delvallez M., Martin G. R. Routine evaluation of the nonradiometric BACTEC NR 660 system. J Clin Microbiol. 1986 Jul;24(1):26–29. doi: 10.1128/jcm.24.1.26-29.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jungkind D., Millan J., Allen S., Dyke J., Hill E. Clinical comparison of a new automated infrared blood culture system with the BACTEC 460 system. J Clin Microbiol. 1986 Feb;23(2):262–266. doi: 10.1128/jcm.23.2.262-266.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Levi M. H., Gialanella P., Motyl M. R., McKitrick J. C. Rapid detection of positive blood cultures with the BACTEC NR-660 does not require first-day subculturing. J Clin Microbiol. 1988 Nov;26(11):2262–2265. doi: 10.1128/jcm.26.11.2262-2265.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Masterson K. C., McGowan J. E., Jr Detection of positive blood cultures by the Bactec NR660. The clinical importance of five versus seven days of testing. Am J Clin Pathol. 1988 Jul;90(1):91–94. doi: 10.1093/ajcp/90.1.91. [DOI] [PubMed] [Google Scholar]
- Morello J. A., Matushek S. M., Dunne W. M., Hinds D. B. Performance of a BACTEC nonradiometric medium for pediatric blood cultures. J Clin Microbiol. 1991 Feb;29(2):359–362. doi: 10.1128/jcm.29.2.359-362.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Elsacker-Niele A. M., Lichtendahl-Bernards A. T., Hermans J., Vandenbroucke J. P., Mouton R. P. Comparison of the automated Bactec NR-660 with a conventional blood culture system. Eur J Clin Microbiol Infect Dis. 1989 Aug;8(8):724–726. doi: 10.1007/BF01963760. [DOI] [PubMed] [Google Scholar]