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. 1992 Apr;30(4):854–857. doi: 10.1128/jcm.30.4.854-857.1992

Comparison of plasmid profiles, phage types, and antimicrobial resistance patterns of Salmonella enteritidis isolates in the United States.

D C Rodrigue 1, D N Cameron 1, N D Puhr 1, F W Brenner 1, M E St Louis 1, I K Wachsmuth 1, R V Tauxe 1
PMCID: PMC265173  PMID: 1572970

Abstract

To evaluate the laboratory techniques for subtyping isolates of Salmonella enteritidis, we compared the plasmid profiles (PP), phage types (PT), and antimicrobial susceptibility patterns (AS) of two nationally representative samples of sporadic human S. enteritidis isolates from 1979 (n = 28) and 1984 (n = 37), 43 isolates from 20 outbreaks of S. enteritidis infections between 1983 and 1987, and 46 animal isolates selected from the U.S. Department of Agriculture Veterinary Services Laboratory in 1986 and 1987. Sporadic and outbreak isolates from humans showed similar rates of resistance to at least one of a panel of antimicrobial drugs (23 and 14%, respectively), PT (91 and 98%, respectively), and PP (97 and 100%, respectively). Sixteen different PP were identified in sporadic, outbreak, and animal isolates; two PP accounted for 76% of sporadic and outbreak isolates. Sporadic human isolates were of PT 8 (42%), of PT 13a (37%), nontypeable (9%), of PT 14b (8%), of PT 9a (3%), and of PT 13 (2%). Outbreak human isolates had similar distributions of PT. PT 8 was associated with poultry: 58% (7 of 12) of the poultry isolates but only 24% (8 of 34) of the isolates from other animals were of PT 8 (P less than 0.04). Although antimicrobial susceptibility patterns do not appear as useful as an epidemiologic marker, PP and PT effectively subtyped S. enteritidis.

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Selected References

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  1. Bauer A. W., Kirby W. M., Sherris J. C., Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966 Apr;45(4):493–496. [PubMed] [Google Scholar]
  2. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Chart H., Threlfall E. J., Rowe B. Virulence of Salmonella enteritidis phage type 4 is related to the possession of a 38 MDa plasmid. FEMS Microbiol Lett. 1989 Apr;49(2-3):299–303. doi: 10.1016/0378-1097(89)90057-8. [DOI] [PubMed] [Google Scholar]
  4. Coyle E. F., Palmer S. R., Ribeiro C. D., Jones H. I., Howard A. J., Ward L., Rowe B. Salmonella enteritidis phage type 4 infection: association with hen's eggs. Lancet. 1988 Dec 3;2(8623):1295–1297. doi: 10.1016/s0140-6736(88)92902-9. [DOI] [PubMed] [Google Scholar]
  5. Frost J. A., Ward L. R., Rowe B. Acquisition of a drug resistance plasmid converts Salmonella enteritidis phage type 4 to phage type 24. Epidemiol Infect. 1989 Oct;103(2):243–248. doi: 10.1017/s0950268800030594. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Gast R. K., Beard C. W. Production of Salmonella enteritidis-contaminated eggs by experimentally infected hens. Avian Dis. 1990 Apr-Jun;34(2):438–446. [PubMed] [Google Scholar]
  7. Holmberg S. D., Wachsmuth I. K., Hickman-Brenner F. W., Cohen M. L. Comparison of plasmid profile analysis, phage typing, and antimicrobial susceptibility testing in characterizing Salmonella typhimurium isolates from outbreaks. J Clin Microbiol. 1984 Feb;19(2):100–104. doi: 10.1128/jcm.19.2.100-104.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Khakhria R., Duck D., Lior H. Distribution of Salmonella enteritidis phage types in Canada. Epidemiol Infect. 1991 Feb;106(1):25–32. doi: 10.1017/s0950268800056417. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. MacDonald K. L., Cohen M. L., Hargrett-Bean N. T., Wells J. G., Puhr N. D., Collin S. F., Blake P. A. Changes in antimicrobial resistance of Salmonella isolated from humans in the United States. JAMA. 1987 Sep 18;258(11):1496–1499. [PubMed] [Google Scholar]
  10. Macrina F. L., Kopecko D. J., Jones K. R., Ayers D. J., McCowen S. M. A multiple plasmid-containing Escherichia coli strain: convenient source of size reference plasmid molecules. Plasmid. 1978 Jun;1(3):417–420. doi: 10.1016/0147-619x(78)90056-2. [DOI] [PubMed] [Google Scholar]
  11. Mayer L. W. Use of plasmid profiles in epidemiologic surveillance of disease outbreaks and in tracing the transmission of antibiotic resistance. Clin Microbiol Rev. 1988 Apr;1(2):228–243. doi: 10.1128/cmr.1.2.228. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Meyers J. A., Sanchez D., Elwell L. P., Falkow S. Simple agarose gel electrophoretic method for the identification and characterization of plasmid deoxyribonucleic acid. J Bacteriol. 1976 Sep;127(3):1529–1537. doi: 10.1128/jb.127.3.1529-1537.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Riley L. W., Cohen M. L., Seals J. E., Blaser M. J., Birkness K. A., Hargrett N. T., Martin S. M., Feldman R. A. Importance of host factors in human salmonellosis caused by multiresistant strains of Salmonella. J Infect Dis. 1984 Jun;149(6):878–883. doi: 10.1093/infdis/149.6.878. [DOI] [PubMed] [Google Scholar]
  14. Rodrigue D. C., Tauxe R. V., Rowe B. International increase in Salmonella enteritidis: a new pandemic? Epidemiol Infect. 1990 Aug;105(1):21–27. doi: 10.1017/s0950268800047609. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. St Louis M. E., Morse D. L., Potter M. E., DeMelfi T. M., Guzewich J. J., Tauxe R. V., Blake P. A. The emergence of grade A eggs as a major source of Salmonella enteritidis infections. New implications for the control of salmonellosis. JAMA. 1988 Apr 8;259(14):2103–2107. [PubMed] [Google Scholar]
  16. Taylor D. N., Wachsmuth I. K., Shangkuan Y. H., Schmidt E. V., Barrett T. J., Schrader J. S., Scherach C. S., McGee H. B., Feldman R. A., Brenner D. J. Salmonellosis associated with marijuana: a multistate outbreak traced by plasmid fingerprinting. N Engl J Med. 1982 May 27;306(21):1249–1253. doi: 10.1056/NEJM198205273062101. [DOI] [PubMed] [Google Scholar]
  17. Threlfall E. J., Rowe B., Ward L. R. Subdivision of Salmonella enteritidis phage types by plasmid profile typing. Epidemiol Infect. 1989 Jun;102(3):459–465. doi: 10.1017/s095026880003017x. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Wachsmuth K. Molecular epidemiology of bacterial infections: examples of methodology and of investigations of outbreaks. Rev Infect Dis. 1986 Sep-Oct;8(5):682–692. doi: 10.1093/clinids/8.5.682. [DOI] [PubMed] [Google Scholar]
  19. Ward L. R., de Sa J. D., Rowe B. A phage-typing scheme for Salmonella enteritidis. Epidemiol Infect. 1987 Oct;99(2):291–294. doi: 10.1017/s0950268800067765. [DOI] [PMC free article] [PubMed] [Google Scholar]

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