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. 1992 Apr;30(4):858–861. doi: 10.1128/jcm.30.4.858-861.1992

Serologic confirmation of simian T-lymphotropic virus type I infection by using immunoassays developed for human T-lymphotropic virus antibody detection.

D L Rudolph 1, J Yee 1, J Mone 1, S K Foung 1, J J Lipka 1, G R Reyes 1, K Hadlock 1, L Chan 1, F Villinger 1, M D Lairmore 1, et al.
PMCID: PMC265174  PMID: 1349306

Abstract

Serum specimens from diverse species of Old World monkeys, categorized as seropositive (n = 97) or seronegative (n = 23) for human T-lymphotropic virus (HTLV) infection, were tested by using recombinant env-spiked Western immunoblot assays and synthetic peptide assays for simultaneous detection and discrimination of simian T-lymphotropic virus (STLV) infection. Of the 97 seropositive specimens, 93 reacted with the recombinant transmembrane (r21env) protein and 90 reacted with a recombinant, MTA-1, derived from the central region of the external glycoprotein of HTLV-I (rgp46env), thus yielding test sensitivities of 96 and 93%, respectively. While 1 of the 23 negative monkey specimens reacted with r21env, none reacted with rgp46env, for overall specificities of 96 and 100%, respectively. Analysis of synthetic peptide-based immunoassays demonstrated that while 85 of 97 (88%) seropositive specimens reacted with HTLV-I-specific epitope (p19gag), none of the specimens reacted with HTLV-II-specific epitope (gp52env). These results show that recombinant envelope-spiked Western blots provide a simple means for serologic confirmation of STLV-I infection and that type-specific synthetic peptides can be used to confirm the virus type in seropositive monkey specimens.

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Selected References

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  1. Anderson D. W., Epstein J. S., Lee T. H., Lairmore M. D., Saxinger C., Kalyanaraman V. S., Slamon D., Parks W., Poiesz B. J., Pierik L. T. Serological confirmation of human T-lymphotropic virus type I infection in healthy blood and plasma donors. Blood. 1989 Nov 15;74(7):2585–2591. [PubMed] [Google Scholar]
  2. Andrada-Serpa M. J., Tosswill J., Schor D., Linhares D., Dobbin J., Pereira M. S. Seroepidemiologic survey for antibodies to human retroviruses in human and non-human primates in Brazil. Int J Cancer. 1989 Sep 15;44(3):389–393. doi: 10.1002/ijc.2910440302. [DOI] [PubMed] [Google Scholar]
  3. Blakeslee J. R., Jr, McClure H. M., Anderson D. C., Bauer R. M., Huff L. Y., Olsen R. G. Chronic fatal disease in gorillas seropositive for simian T-lymphotropic virus I antibodies. Cancer Lett. 1987 Oct;37(1):1–6. doi: 10.1016/0304-3835(87)90139-x. [DOI] [PubMed] [Google Scholar]
  4. Chen Y. M., Lee T. H., Samuel K. P., Okayama A., Tachibana N., Miyoshi I., Papas T. S., Essex M. Antibody reactivity to different regions of human T-cell leukemia virus type 1 gp61 in infected people. J Virol. 1989 Nov;63(11):4952–4957. doi: 10.1128/jvi.63.11.4952-4957.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Coates S. R., Harris A. J., Parkes D. L., Smith C. M., Liu H. L., Akita R. W., Ferrer M. M., Sampson E. K., Brandis J. W., Sliwkowski M. X. Serological evaluation of Escherichia coli-expressed human T-cell leukemia virus type I env, gag p24, and tax proteins. J Clin Microbiol. 1990 Jun;28(6):1139–1142. doi: 10.1128/jcm.28.6.1139-1142.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Dracopoli N. C., Turner T. R., Else J. G., Jolly C. J., Anthony R., Gallo R. C., Saxinger W. C. STLV-I antibodies in feral populations of East African vervet monkeys (Cercopithecus aethiops). Int J Cancer. 1986 Oct 15;38(4):523–529. doi: 10.1002/ijc.2910380412. [DOI] [PubMed] [Google Scholar]
  7. Fultz P. N., Gordon T. P., Anderson D. C., McClure H. M. Prevalence of natural infection with simian immunodeficiency virus and simian T-cell leukemia virus type I in a breeding colony of sooty mangabey monkeys. AIDS. 1990 Jul;4(7):619–625. doi: 10.1097/00002030-199007000-00002. [DOI] [PubMed] [Google Scholar]
  8. Hartley T. M., Khabbaz R. F., Cannon R. O., Kaplan J. E., Lairmore M. D. Characterization of antibody reactivity to human T-cell lymphotropic virus types I and II using immunoblot and radioimmunoprecipitation assays. J Clin Microbiol. 1990 Apr;28(4):646–650. doi: 10.1128/jcm.28.4.646-650.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Hartley T. M., Malone G. E., Khabbaz R. F., Lal R. B., Kaplan J. E. Evaluation of a recombinant human T-cell lymphotropic virus type I (HTLV-I) p21E antibody detection enzyme immunoassay as a supplementary test in HTLV-I/II antibody testing algorithms. J Clin Microbiol. 1991 Jun;29(6):1125–1127. doi: 10.1128/jcm.29.6.1125-1127.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Homma T., Kanki P. J., King N. W., Jr, Hunt R. D., O'Connell M. J., Letvin N. L., Daniel M. D., Desrosiers R. C., Yang C. S., Essex M. Lymphoma in macaques: association with virus of human T lymphotrophic family. Science. 1984 Aug 17;225(4663):716–718. doi: 10.1126/science.6087453. [DOI] [PubMed] [Google Scholar]
  11. Inoue J., Watanabe T., Sato M., Oda A., Toyoshima K., Yoshida M., Seiki M. Nucleotide sequence of the protease-coding region in an infectious DNA of simian retrovirus (STLV) of the HTLV-I family. Virology. 1986 Apr 15;150(1):187–195. doi: 10.1016/0042-6822(86)90278-3. [DOI] [PubMed] [Google Scholar]
  12. Ishikawa K., Fukasawa M., Tsujimoto H., Else J. G., Isahakia M., Ubhi N. K., Ishida T., Takenaka O., Kawamoto Y., Shotake T. Serological survey and virus isolation of simian T-cell leukemia/T-lymphotropic virus type I (STLV-I) in non-human primates in their native countries. Int J Cancer. 1987 Aug 15;40(2):233–239. doi: 10.1002/ijc.2910400219. [DOI] [PubMed] [Google Scholar]
  13. Lairmore M. D., Lerche N. W., Schultz K. T., Stone C. M., Brown B. G., Hermann L. M., Yee J. A., Jennings M. SIV, STLV-I and type D retrovirus antibodies in captive rhesus macaques and immunoblot reactivity to SIV p27 in human and rhesus monkey sera. AIDS Res Hum Retroviruses. 1990 Nov;6(11):1233–1238. doi: 10.1089/aid.1990.6.1233. [DOI] [PubMed] [Google Scholar]
  14. Lal R. B., Griffis K. P. Predictive B- and T-cell linear epitopes in structural proteins of HTLV-I, HTLV-II, and STLV-I. AIDS Res Hum Retroviruses. 1991 Aug;7(8):663–670. doi: 10.1089/aid.1991.7.663. [DOI] [PubMed] [Google Scholar]
  15. Lal R. B., Heneine W., Rudolph D. L., Present W. B., Hofhienz D., Hartley T. M., Khabbaz R. F., Kaplan J. E. Synthetic peptide-based immunoassays for distinguishing between human T-cell lymphotropic virus type I and type II infections in seropositive individuals. J Clin Microbiol. 1991 Oct;29(10):2253–2258. doi: 10.1128/jcm.29.10.2253-2258.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Lal R. B., Rudolph D. L., Lairmore M. D., Khabbaz R. F., Garfield M., Coligan J. E., Folks T. M. Serologic discrimination of human T cell lymphotropic virus infection by using a synthetic peptide-based enzyme immunoassay. J Infect Dis. 1991 Jan;163(1):41–46. doi: 10.1093/infdis/163.1.41. [DOI] [PubMed] [Google Scholar]
  17. Lillehoj E. P., Alexander S. S., Dubrule C. J., Wiktor S., Adams R., Tai C. C., Manns A., Blattner W. A. Development and evaluation of a human T-cell leukemia virus type I serologic confirmatory assay incorporating a recombinant envelope polypeptide. J Clin Microbiol. 1990 Dec;28(12):2653–2658. doi: 10.1128/jcm.28.12.2653-2658.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lipka J. J., Santiago P., Chan L., Reyes G. R., Samuel K. P., Blattner W. A., Shaw G. M., Hanson C. V., Sninsky J. J., Foung S. K. Modified Western blot assay for confirmation and differentiation of human T cell lymphotropic virus types I and II. J Infect Dis. 1991 Aug;164(2):400–403. doi: 10.1093/infdis/164.2.400. [DOI] [PubMed] [Google Scholar]
  19. McCarthy T. J., Kennedy J. L., Blakeslee J. R., Bennett B. T. Spontaneous malignant lymphoma and leukemia in a simian T-lymphotropic virus type I (STLV-I) antibody positive olive baboon. Lab Anim Sci. 1990 Jan;40(1):79–81. [PubMed] [Google Scholar]
  20. Noda Y., Ishikawa K., Sasagawa A., Honjo S., Mori S., Tsujimoto H., Hayami M. Hematologic abnormalities similar to the preleukemic state of adult T-cell leukemia in African green monkeys naturally infected with simian T-cell leukemia virus. Jpn J Cancer Res. 1986 Dec;77(12):1227–1234. [PubMed] [Google Scholar]
  21. Rudolph D. L., Keesling S. S., Lerche N., Yee J. A., Lal R. Dominance of HTLV type I-specific antibody responsiveness in Old World monkeys. AIDS Res Hum Retroviruses. 1991 Sep;7(9):721–722. doi: 10.1089/aid.1991.7.721. [DOI] [PubMed] [Google Scholar]
  22. Sakakibara I., Sugimoto Y., Sasagawa A., Honjo S., Tsujimoto H., Nakamura H., Hayami M. Spontaneous malignant lymphoma in an African green monkey naturally infected with simian T-lymphotropic virus (STLV). J Med Primatol. 1986;15(5):311–318. [PubMed] [Google Scholar]
  23. Tsujimoto H., Noda Y., Ishikawa K., Nakamura H., Fukasawa M., Sakakibara I., Sasagawa A., Honjo S., Hayami M. Development of adult T-cell leukemia-like disease in African green monkey associated with clonal integration of simian T-cell leukemia virus type I. Cancer Res. 1987 Jan 1;47(1):269–274. [PubMed] [Google Scholar]
  24. Watanabe T., Seiki M., Tsujimoto H., Miyoshi I., Hayami M., Yoshida M. Sequence homology of the simian retrovirus genome with human T-cell leukemia virus type I. Virology. 1985 Jul 15;144(1):59–65. doi: 10.1016/0042-6822(85)90304-6. [DOI] [PubMed] [Google Scholar]

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