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. 1992 Apr;30(4):1005–1007. doi: 10.1128/jcm.30.4.1005-1007.1992

Highly sensitive enhanced chemiluminescence immunodetection method for herpes simplex virus type 2 Western immunoblot.

J Dalessio 1, R Ashley 1
PMCID: PMC265202  PMID: 1315326

Abstract

Three Western blot (immunoblot) methods for detecting antibodies to herpes simplex virus type 2 were compared: (i) nitrocellulose blots with 4-chloro-1-naphthol immunostaining (4CN-WB); (ii) polyvinylidene difluoride (PVDF) blots with 3,3',5,5'-tetramethylbenzidene immunostaining (TMB-WB); and (iii) PVDF blots with enhanced chemiluminescence (ECL-WB). TMB-WB was 10-fold more sensitive than 4CN-WB, while ECL-WB was as much as 500-fold more sensitive.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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