Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Apr;30(4):1014–1015. doi: 10.1128/jcm.30.4.1014-1015.1992

Comparison of immunotyping of Chlamydia trachomatis by indirect fluorescent-antibody staining and radioimmunoassay.

B J van der Pol 1, R B Jones 1
PMCID: PMC265205  PMID: 1572957

Abstract

Chlamydia trachomatis isolates from 58 patients were immunotyped by radioimmunoassay (RIA) and fluorescent-antibody staining (FA) by using monoclonal antibodies. Unambiguous typing was observed with 55 isolates by FA and 53 isolates by RIA, although 3 isolates were typed by RIA but not FA. FA required less antigen and was more sensitive in detecting mixed infections.

Full text

PDF
1014

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Barnes R. C., Rompalo A. M., Stamm W. E. Comparison of Chlamydia trachomatis serovars causing rectal and cervical infections. J Infect Dis. 1987 Dec;156(6):953–958. doi: 10.1093/infdis/156.6.953. [DOI] [PubMed] [Google Scholar]
  2. Barnes R. C., Wang S. P., Kuo C. C., Stamm W. E. Rapid immunotyping of Chlamydia trachomatis with monoclonal antibodies in a solid-phase enzyme immunoassay. J Clin Microbiol. 1985 Oct;22(4):609–613. doi: 10.1128/jcm.22.4.609-613.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Batteiger B. E., Fraiz J., Newhall W. J., Katz B. P., Jones R. B. Association of recurrent chlamydial infection with gonorrhea. J Infect Dis. 1989 Apr;159(4):661–669. doi: 10.1093/infdis/159.4.661. [DOI] [PubMed] [Google Scholar]
  4. Newhall W. J., 5th, Terho P., Wilde C. E., 3rd, Batteiger B. E., Jones R. B. Serovar determination of Chlamydia trachomatis isolates by using type-specific monoclonal antibodies. J Clin Microbiol. 1986 Feb;23(2):333–338. doi: 10.1128/jcm.23.2.333-338.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Wang S. P., Grayston J. T. Chlamydia pneumoniae elementary body antigenic reactivity with fluorescent antibody is destroyed by methanol. J Clin Microbiol. 1991 Jul;29(7):1539–1541. doi: 10.1128/jcm.29.7.1539-1541.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Wang S. P., Grayston J. T. Serotyping of Chlamydia trachomatis by indirect fluorescent-antibody staining of inclusions in cell culture with monoclonal antibodies. J Clin Microbiol. 1991 Jul;29(7):1295–1298. doi: 10.1128/jcm.29.7.1295-1298.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Wang S. P., Grayston J. T. Three new serovars of Chlamydia trachomatis: Da, Ia, and L2a. J Infect Dis. 1991 Feb;163(2):403–405. doi: 10.1093/infdis/163.2.403. [DOI] [PubMed] [Google Scholar]
  8. Wang S. P., Kuo C. C., Barnes R. C., Stephens R. S., Grayston J. T. Immunotyping of Chlamydia trachomatis with monoclonal antibodies. J Infect Dis. 1985 Oct;152(4):791–800. doi: 10.1093/infdis/152.4.791. [DOI] [PubMed] [Google Scholar]
  9. Wang S. P., Kuo C. C., Grayston J. T. A simplified method for immunological typing of trachoma-inclusion conjunctivitis-lymphogranuloma venereum organisms. Infect Immun. 1973 Mar;7(3):356–360. doi: 10.1128/iai.7.3.356-360.1973. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES