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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Apr;30(4):1019–1021. doi: 10.1128/jcm.30.4.1019-1021.1992

New Granada Medium for detection and identification of group B streptococci.

M de la Rosa 1, M Perez 1, C Carazo 1, L Pareja 1, J I Peis 1, F Hernandez 1
PMCID: PMC265207  PMID: 1572958

Abstract

A methotrexate-containing medium for the detection of beta-hemolytic group B streptococci from clinical specimens on the basis of detection of pigment is described. The medium contained peptone, starch, serum, MgSO4, glucose, pyruvate, methotrexate (as pigment enhancer), phosphate-morpholine-propanesulfonic acid buffer, and selective agents. The recovery of beta-hemolytic group B streptococci was comparable to that obtained with selective broth.

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Selected References

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  1. Ascher D. P., Wilson S., Fischer G. W. Comparison of commercially available group B streptococcal latex agglutination assays. J Clin Microbiol. 1991 Dec;29(12):2895–2896. doi: 10.1128/jcm.29.12.2895-2896.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. De La Rosa M., Villareal R., Vega D., Miranda C., Martinezbrocal A. Granada medium for detection and identification of group B streptococci. J Clin Microbiol. 1983 Oct;18(4):779–785. doi: 10.1128/jcm.18.4.779-785.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Islam A. K. Rapid recognition of group-B Streptococci. Lancet. 1977 Jan 29;1(8005):256–257. doi: 10.1016/s0140-6736(77)91055-8. [DOI] [PubMed] [Google Scholar]
  4. Merritt K., Jacobs N. J. Characterization and incidence of pigment production by human clinical group B streptococci. J Clin Microbiol. 1978 Jul;8(1):105–107. doi: 10.1128/jcm.8.1.105-107.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Merritt K., Jacobs N. J. Improved medium for detecting pigment production by group B streptococci. J Clin Microbiol. 1976 Oct;4(4):379–380. doi: 10.1128/jcm.4.4.379-380.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Noble M. A., Bent J. M., West A. B. Detection and identification of group B streptococci by use of pigment production. J Clin Pathol. 1983 Mar;36(3):350–352. doi: 10.1136/jcp.36.3.350. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Persson K. M., Forsgren A. Evaluation of culture methods for isolation of group B streptococci. Diagn Microbiol Infect Dis. 1987 Feb;6(2):175–177. doi: 10.1016/0732-8893(87)90104-0. [DOI] [PubMed] [Google Scholar]
  8. Reardon E. P., Noble M. A., Luther E. R., Wort A. J., Bent J., Swift M. Evaluation of a rapid method for the detection of vaginal group B streptococci in women in labor. Am J Obstet Gynecol. 1984 Mar 1;148(5):575–578. doi: 10.1016/0002-9378(84)90751-8. [DOI] [PubMed] [Google Scholar]
  9. Tapsall J. W. Pigment production by Lancefield-group-B streptococci (Streptococcus agalactiae). J Med Microbiol. 1986 Feb;21(1):75–81. doi: 10.1099/00222615-21-1-75. [DOI] [PubMed] [Google Scholar]
  10. Waitkins S. A. A selective and differential medium for group B streptococci. Med Lab Sci. 1982 Apr;39(2):185–188. [PubMed] [Google Scholar]
  11. Wang E. E., Hammerberg O., Lyn P., Peng H. Q., Hunter D., Richardson H. Rapid detection of group B streptococcal carriage in parturient women using a modified starch serum medium. Clin Invest Med. 1988 Feb;11(1):52–56. [PubMed] [Google Scholar]
  12. Wang E., Richardson H. A rapid method for detection of group B streptococcal colonization: testing at the bedside. Obstet Gynecol. 1990 Nov;76(5 Pt 1):882–885. doi: 10.1097/00006250-199011000-00033. [DOI] [PubMed] [Google Scholar]

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