Abstract
Two monoclonal antibodies (MAbs) of the immunoglobulin G2A isotype, reacting with a Nocardia-specific 54-kDa antigen, were generated. As determined by Western blot (immunoblot), both MAbs reacted only with the 54-kDa band. As determined by indirect immunofluorescence or enzyme immunoassay with whole microorganisms, the MAbs did not react with Nocardia cells. One of the MAbs showed weak cross-reactivity with mycobacterial antigens, while the other showed no cross-reactivity.
Full text
PDFImages in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Angeles A. M., Sugar A. M. Identification of a common immunodominant protein in culture filtrates of three Nocardia species and use in etiologic diagnosis of mycetoma. J Clin Microbiol. 1987 Dec;25(12):2278–2280. doi: 10.1128/jcm.25.12.2278-2280.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Baddour L. M., Baselski V. S., Herr M. J., Christensen G. D., Bisno A. L. Nocardiosis in recipients of renal transplants: evidence for nosocomial acquisition. Am J Infect Control. 1986 Oct;14(5):214–219. doi: 10.1016/0196-6553(86)90120-3. [DOI] [PubMed] [Google Scholar]
- Bazin H., Cormont F., De Clercq L. Rat monoclonal antibodies. II. A rapid and efficient method of purification from ascitic fluid or serum. J Immunol Methods. 1984 Jun 8;71(1):9–16. doi: 10.1016/0022-1759(84)90200-x. [DOI] [PubMed] [Google Scholar]
- Blumer S. O., Kaufman L. Microimmunodiffusion test for nocardiosis. J Clin Microbiol. 1979 Sep;10(3):308–312. doi: 10.1128/jcm.10.3.308-312.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Boiron P., Provost F. Use of partially purified 54-kilodalton antigen for diagnosis of nocardiosis by Western blot (immunoblot) assay. J Clin Microbiol. 1990 Feb;28(2):328–331. doi: 10.1128/jcm.28.2.328-331.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Humphreys D. W., Crowder J. G., White A. Serological reactions to Nocardia antigens. Am J Med Sci. 1975 May-Jun;269(3):323–326. doi: 10.1097/00000441-197505000-00005. [DOI] [PubMed] [Google Scholar]
- Jiménez T., Díaz A. M., Zlotnik H. Monoclonal antibodies to Nocardia asteroides and Nocardia brasiliensis antigens. J Clin Microbiol. 1990 Jan;28(1):87–91. doi: 10.1128/jcm.28.1.87-91.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kints J. P., Manouvriez P., Bazin H. Rat monoclonal antibodies. VII. Enhancement of ascites production and yield of monoclonal antibodies in rats following pretreatment with pristane and Freund's adjuvant. J Immunol Methods. 1989 May 12;119(2):241–245. doi: 10.1016/0022-1759(89)90402-x. [DOI] [PubMed] [Google Scholar]
- Matthews R., Burnie J. Diagnosis of systemic candidiasis by an enzyme-linked dot immunobinding assay for a circulating immunodominant 47-kilodalton antigen. J Clin Microbiol. 1988 Mar;26(3):459–463. doi: 10.1128/jcm.26.3.459-463.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rajki K., Brehmer W., Hammer H. J., Fischer W., Daus H., Mauch H. Analysis of the soluble cytoplasmic components of Mycobacteria and Nocardia by crossed immunoelectrofocusing. Zentralbl Bakteriol Mikrobiol Hyg A. 1982 Mar;251(3):389–398. [PubMed] [Google Scholar]
- Rosendale D. E., Myers C., Boyko E. J., Jafek B. Nocardia asteroides cervical osteomyelitis in an immunocompetent host. Otolaryngol Head Neck Surg. 1988 Sep;99(3):334–337. doi: 10.1177/019459988809900313. [DOI] [PubMed] [Google Scholar]
- Shainhouse J. Z., Pier A. C., Stevens D. A. Complement fixation antibody test for human nocardiosis. J Clin Microbiol. 1978 Nov;8(5):516–519. doi: 10.1128/jcm.8.5.516-519.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Sugar A. M., Schoolnik G. K., Stevens D. A. Antibody response in human nocardiosis: identification of two immunodominant culture-filtrate antigens derived from Nocardia asteroides. J Infect Dis. 1985 May;151(5):895–901. doi: 10.1093/infdis/151.5.895. [DOI] [PubMed] [Google Scholar]
- el-Zaatari F. A., Reiss E., Yakrus M. A., Bragg S. L., Kaufman L. Monoclonal antibodies against isoelectrically focused Nocardia asteroides proteins characterized by the enzyme-linked immunoelectro-transfer blot method. Diagn Immunol. 1986;4(2):97–106. [PubMed] [Google Scholar]