Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 May;30(5):1174–1178. doi: 10.1128/jcm.30.5.1174-1178.1992

Indirect hemagglutination assay for diagnosis of Escherichia coli O157 infection in patients with hemolytic-uremic syndrome.

M Bitzan 1, H Karch 1
PMCID: PMC265245  PMID: 1583116

Abstract

An indirect hemagglutination assay consisting of sheep erythrocytes coated with lipopolysaccharide (LPS) from Shiga-like toxin-producing Escherichia coli O157 was used for the serological diagnosis of E. coli O157 infections in children with classical (enteropathic) hemolytic-uremic syndrome (HUS). One week after the onset of diarrhea (acute phase of the disease), the E. coli O157 antibody titer was greater than or equal to 1:4,096 in 22 of 27 patients with HUS, compared with 4 of 249 controls, the majority of whom had O157 antibody titers of between 1:4 and 1:256. This antibody response was observed in HUS patients with stool cultures positive and negative for E. coli O157. Selective absorption with homologous LPS and heterologous LPS showed that the antibody response was specific for E. coli O157. Because of its simplicity and ease of interpretation, the indirect hemagglutination assay described in this paper is recommended for the serological diagnosis of E. coli O157 infections in patients with HUS.

Full text

PDF
1174

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bitzan M., Häck H. J., Mauff G. Yersinia enterocolitica serodiagnosis: a dual role of specific IgA. Evaluation of microagglutination and ELISA. Zentralbl Bakteriol Mikrobiol Hyg A. 1987 Dec;267(2):194–205. doi: 10.1016/s0176-6724(87)80005-6. [DOI] [PubMed] [Google Scholar]
  2. Bitzan M., Moebius E., Ludwig K., Müller-Wiefel D. E., Heesemann J., Karch H. High incidence of serum antibodies to Escherichia coli O157 lipopolysaccharide in children with hemolytic-uremic syndrome. J Pediatr. 1991 Sep;119(3):380–385. doi: 10.1016/s0022-3476(05)82049-9. [DOI] [PubMed] [Google Scholar]
  3. Chart H., Smith H. R., Scotland S. M., Rowe B., Milford D. V., Taylor C. M. Serological identification of Escherichia coli O157:H7 infection in haemolytic uraemic syndrome. Lancet. 1991 Jan 19;337(8734):138–140. doi: 10.1016/0140-6736(91)90801-u. [DOI] [PubMed] [Google Scholar]
  4. Cleary T. G., Lopez E. L. The shiga-like toxin-producing Escherichia coli and hemolytic uremic syndrome. Pediatr Infect Dis J. 1989 Oct;8(10):720–724. doi: 10.1097/00006454-198910000-00012. [DOI] [PubMed] [Google Scholar]
  5. Del Guercio P., Tolone G., Andrade F. B., Biozzi G., Binaghi R. A. Opsonic, cytophilic and agglutinating activity of guinea-pig gamma-2 and gamma-M anti-Salmonella antibodies. Immunology. 1969 Mar;16(3):361–371. [PMC free article] [PubMed] [Google Scholar]
  6. Jodal U. The immune response to urinary tract infections in childhood. I. Serological diagnosis of primary symptomatic infection in girls by indirect hemagglutination. Acta Paediatr Scand. 1975 Jan;64(1):96–104. [PubMed] [Google Scholar]
  7. Karch H., Wiss R., Gloning H., Emmrich P., Aleksić S., Bockemühl J. Hämolytisch-urämisches Syndrom bei Kleinkindern durch Verotoxin-produzierende Escherichia coli. Dtsch Med Wochenschr. 1990 Mar 30;115(13):489–495. doi: 10.1055/s-2008-1065036. [DOI] [PubMed] [Google Scholar]
  8. Karmali M. A., Arbus G. S., Petric M., Patrick M. L., Roscoe M., Shaw J., Lior H. Hospital-acquired Escherichia coli O157:H7 associated haemolytic uraemic syndrome in a nurse. Lancet. 1988 Mar 5;1(8584):526–526. doi: 10.1016/s0140-6736(88)91310-4. [DOI] [PubMed] [Google Scholar]
  9. Karmali M. A. Infection by verocytotoxin-producing Escherichia coli. Clin Microbiol Rev. 1989 Jan;2(1):15–38. doi: 10.1128/cmr.2.1.15. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Karmali M. A., Steele B. T., Petric M., Lim C. Sporadic cases of haemolytic-uraemic syndrome associated with faecal cytotoxin and cytotoxin-producing Escherichia coli in stools. Lancet. 1983 Mar 19;1(8325):619–620. doi: 10.1016/s0140-6736(83)91795-6. [DOI] [PubMed] [Google Scholar]
  11. Lopez E. L., Diaz M., Devoto S., Grinstein S., Woloj M., Murray B. E., Rubeglio E., Mendilaharzu F., Turco M., Vasquez M. Evidence of infection with organisms producing Shiga-like toxins in household contacts of children with the hemolytic uremic syndrome. Pediatr Infect Dis J. 1991 Jan;10(1):20–24. doi: 10.1097/00006454-199101000-00005. [DOI] [PubMed] [Google Scholar]
  12. Perry M. B., Bundle D. R. Antigenic relationships of the lipopolysaccharides of Escherichia hermannii strains with those of Escherichia coli O157:H7, Brucella melitensis, and Brucella abortus. Infect Immun. 1990 May;58(5):1391–1395. doi: 10.1128/iai.58.5.1391-1395.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Schlecht S., Westphal O. Wachstum und Lipopolysaccharid (O-Antigen)-Gehalt von Salmonellen bei Züchtung auf Agarnährböden. Zentralbl Bakteriol Orig. 1966 Jun;200(2):241–259. [PubMed] [Google Scholar]
  14. Tarr P. I., Neill M. A., Clausen C. R., Watkins S. L., Christie D. L., Hickman R. O. Escherichia coli O157:H7 and the hemolytic uremic syndrome: importance of early cultures in establishing the etiology. J Infect Dis. 1990 Aug;162(2):553–556. doi: 10.1093/infdis/162.2.553. [DOI] [PubMed] [Google Scholar]
  15. Vosti K. L., Monto A. S., Rantz L. A. Host-parasite interaction in patients with infections due to Escherichia coli. II. Serologic response of the host. J Lab Clin Med. 1965 Oct;66(4):613–626. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES