Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 May;30(5):1194–1197. doi: 10.1128/jcm.30.5.1194-1197.1992

Novel immunoenzymatic assay for identification of coagulase- and protein A-negative Staphylococcus aureus strains.

C A Guzmàn 1, M C Guardati 1, D Fenoglio 1, G Coratza 1, C Pruzzo 1, G Satta 1
PMCID: PMC265249  PMID: 1583119

Abstract

A purified monoclonal antibody (MAb) which specifically reacts with Staphylococcus aureus glucosaminidase was obtained. This MAb was utilized to develop an immunoenzymatic assay for the identification of S. aureus strains. The sensitivity of this assay, based on the simultaneous detection of S. aureus glucosaminidase and protein A, was evaluated by analyzing a total of 196 strains, 26 of which did not exhibit one or more of the following properties: protein A, clumping factor, and staphylocoagulase. All strains yielded positive results by the MAb-based immunoenzymatic test. The assay's ability to differentiate between S. aureus and other staphylococci was then analyzed by testing a total of 277 non-S. aureus strains that yielded negative results. Our data demonstrate that this immunoenzymatic assay can be used as a single S. aureus identification criterion, particularly useful for those strains negative for clumping factor, staphylocoagulase, or protein A.

Full text

PDF
1194

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Accolla R. S., Cina R., Montesoro E., Celada F. Antibody-mediated activation of genetically defective Escherichia coli beta-galactosidases by monoclonal antibodies produced by somatic cell hybrids. Proc Natl Acad Sci U S A. 1981 Apr;78(4):2478–2482. doi: 10.1073/pnas.78.4.2478. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Aldridge K. E., Kogos C., Sanders C. V., Marier R. L. Comparison of rapid identification assays for Staphylococcus aureus. J Clin Microbiol. 1984 May;19(5):703–704. doi: 10.1128/jcm.19.5.703-704.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Baker J. S., Bormann M. A., Boudreau D. H. Evaluation of various rapid agglutination methods for the identification of Staphylococcus aureus. J Clin Microbiol. 1985 May;21(5):726–729. doi: 10.1128/jcm.21.5.726-729.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Berke A., Tilton R. C. Evaluation of rapid coagulase methods for the identification of Staphylococcus aureus. J Clin Microbiol. 1986 May;23(5):916–919. doi: 10.1128/jcm.23.5.916-919.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brown W. J. Comparison of a yellow latex reagent with other agglutination methods for the identification of Staphylococcus aureus. J Clin Microbiol. 1986 Mar;23(3):640–642. doi: 10.1128/jcm.23.3.640-642.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Forsgren A. Significance of protein a production by staphylococci. Infect Immun. 1970 Nov;2(5):672–673. doi: 10.1128/iai.2.5.672-673.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Kearney J. F., Radbruch A., Liesegang B., Rajewsky K. A new mouse myeloma cell line that has lost immunoglobulin expression but permits the construction of antibody-secreting hybrid cell lines. J Immunol. 1979 Oct;123(4):1548–1550. [PubMed] [Google Scholar]
  8. Kronvall G., Dossett J. H., Quie P. G., Williams R. C. Occurrence of protein a in staphylococcal strains: quantitative aspects and correlation to antigenic and bacteriophage types. Infect Immun. 1971 Jan;3(1):10–15. doi: 10.1128/iai.3.1.10-15.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Lindmark R., Movitz J., Sjöquist J. Extracellular protein A from a methicillin-resistant strain of Staphylococcus aureus. Eur J Biochem. 1977 Apr 15;74(3):623–628. doi: 10.1111/j.1432-1033.1977.tb11431.x. [DOI] [PubMed] [Google Scholar]
  10. Maxim P. E., Mathews H. L., Mengoli H. F. Single-tube mixed agglutination test for the detection of staphylococcal protein A. J Clin Microbiol. 1976 Nov;4(5):418–422. doi: 10.1128/jcm.4.5.418-422.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Orth D. S., Chugg L. R., Anderson A. W. Comparison of animal sera for suitability in coagulase testing. Appl Microbiol. 1971 Mar;21(3):420–425. doi: 10.1128/am.21.3.420-425.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Pompei R., Thaller M. C., Pittaluga F., Flore O., Satta G. Analysis of bacteriolytic activity patterns, a novel approach to the taxonomy of enterococci. Int J Syst Bacteriol. 1992 Jan;42(1):37–43. doi: 10.1099/00207713-42-1-37. [DOI] [PubMed] [Google Scholar]
  13. Sandermann H., Jr, Strominger J. L. Purification and properties of C 55 -isoprenoid alcohol phosphokinase from Staphylococcus aureus. J Biol Chem. 1972 Aug 25;247(16):5123–5131. [PubMed] [Google Scholar]
  14. Satta G., Varaldo P. E., Grazi G., Fontana R. Bacteriolytic activity in staphylococci. Infect Immun. 1977 Apr;16(1):37–42. doi: 10.1128/iai.16.1.37-42.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Selepak S. T., Witebsky F. G. Inoculum size and lot-to-lot variation as significant variables in the tube coagulase test for Staphylococcus aureus. J Clin Microbiol. 1985 Nov;22(5):835–837. doi: 10.1128/jcm.22.5.835-837.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Sperber W. H., Tatini S. R. Interpretation of the tube coagulase test for identification of Staphylococcus aureus. Appl Microbiol. 1975 Apr;29(4):502–505. doi: 10.1128/am.29.4.502-505.1975. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Valisena S., Varaldo P. E., Satta G. Purification and characterization of three separate bacteriolytic enzymes excreted by Staphylococcus aureus, Staphylococcus simulans, and Staphylococcus saprophyticus. J Bacteriol. 1982 Aug;151(2):636–647. doi: 10.1128/jb.151.2.636-647.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Valisena S., Varaldo P. E., Satta G. Staphylococcal endo-beta-N-acetylglucosaminidase inhibits response of human lymphocytes to mitogens and interferes with production of antibodies in mice. J Clin Invest. 1991 Jun;87(6):1969–1976. doi: 10.1172/JCI115224. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Varaldo P. E., Grazi G., Soro O., Cisani G., Satta G. Simplified lyogroup system, a new method for routine identification of staphylococci: description and comparison with three other methods. J Clin Microbiol. 1980 Jul;12(1):63–68. doi: 10.1128/jcm.12.1.63-68.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Wegrzynowicz Z., Heczko P. B., Jeljaszewicz J., Neugebauer M., Pulverer G. Pseudocoagulase activity of staphylococci. J Clin Microbiol. 1979 Jan;9(1):15–19. doi: 10.1128/jcm.9.1.15-19.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Wilkinson B. J., Maxwell S., Schaus S. M. Classification and characteristics of coagulase-negative, methicillin-resistant staphylococci. J Clin Microbiol. 1980 Aug;12(2):161–166. doi: 10.1128/jcm.12.2.161-166.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Winblad S., Ericson C. Sensitized sheep red cells as a reactant for Staphylococcus aureus protein A. Methodology and epidemiology with special reference to weakly reacting methicillin-resistant strains. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Feb;81(1):150–156. [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES