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. 1992 Jun;30(6):1424–1427. doi: 10.1128/jcm.30.6.1424-1427.1992

Method for detection of simian immunodeficiency virus neutralizing antibodies using a noncommercial antigen capture enzyme-linked immunosorbent assay.

M J Welch 1, M E Thouless 1
PMCID: PMC265303  PMID: 1624558

Abstract

A neutralization test (NT) using a noncommercial antigen capture enzyme-linked immunosorbent assay (ELISA) to detect simian immunodeficiency virus (SIV) growth in vitro was developed. The capture antibody was a mixture of purified macaque anti-SIV immunoglobulin G (IgG) and a monoclonal antibody to SIV p27. Captured antigens were detected by using purified macaque anti-SIV IgG conjugated to horseradish peroxidase. The NT reliably and sensitively detected differences when various amounts of SIV were used with positive and negative control macaque sera. Dilutions of sequential sera from a macaque (Macaca nemestrina) that had been experimentally infected with SIV were tested for neutralizing antibody with 300 50% tissue culture infective doses of SIV. In this macaque, neutralizing activity and anti-SIV IgG levels in serum (detected by ELISA) increased with time after SIV inoculation, and high IgG titers were required in serum before neutralization occurred in vitro. This simple NT, which detects the presence of SIV serum neutralizing antibodies at a low cost, will be useful for investigating the role of neutralizing antibodies in the SIV-infected macaque model for AIDS.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Benveniste R. E., Arthur L. O., Tsai C. C., Sowder R., Copeland T. D., Henderson L. E., Oroszlan S. Isolation of a lentivirus from a macaque with lymphoma: comparison with HTLV-III/LAV and other lentiviruses. J Virol. 1986 Nov;60(2):483–490. doi: 10.1128/jvi.60.2.483-490.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Benveniste R. E., Hill R. W., Eron L. J., Csaikl U. M., Knott W. B., Henderson L. E., Sowder R. C., Nagashima K., Gonda M. A. Characterization of clones of HIV-1 infected HuT 78 cells defective in gag gene processing and of SIV clones producing large amounts of envelope glycoprotein. J Med Primatol. 1990;19(3-4):351–366. [PubMed] [Google Scholar]
  3. Daniel M. D., Sehgal P. K., Kodama T., Wyand M. S., Ringler D. J., King N. W., Schmidt D. K., Troup C. D., Desrosiers R. C. Use of simian immunodeficiency virus for vaccine research. J Med Primatol. 1990;19(3-4):395–399. [PubMed] [Google Scholar]
  4. Desrosiers R. C., Wyand M. S., Kodama T., Ringler D. J., Arthur L. O., Sehgal P. K., Letvin N. L., King N. W., Daniel M. D. Vaccine protection against simian immunodeficiency virus infection. Proc Natl Acad Sci U S A. 1989 Aug;86(16):6353–6357. doi: 10.1073/pnas.86.16.6353. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Groopman J. E., Benz P. M., Ferriani R., Mayer K., Allan J. D., Weymouth L. A. Characterization of serum neutralization response to the human immunodeficiency virus (HIV). AIDS Res Hum Retroviruses. 1987 Spring;3(1):71–85. doi: 10.1089/aid.1987.3.71. [DOI] [PubMed] [Google Scholar]
  6. Haigwood N. L., Shuster J. R., Moore G. K., Lee H., Skiles P. V., Higgins K. W., Barr P. J., George-Nascimento C., Steimer K. S. Importance of hypervariable regions of HIV-1 gp120 in the generation of virus neutralizing antibodies. AIDS Res Hum Retroviruses. 1990 Jul;6(7):855–869. doi: 10.1089/aid.1990.6.855. [DOI] [PubMed] [Google Scholar]
  7. Harada S., Purtilo D. T., Koyanagi Y., Sonnabend J., Yamamoto N. Sensitive assay for neutralizing antibodies against AIDS-related viruses (HTLV-III/LAV). J Immunol Methods. 1986 Sep 27;92(2):177–181. doi: 10.1016/0022-1759(86)90163-8. [DOI] [PubMed] [Google Scholar]
  8. Ho D. D., Sarngadharan M. G., Hirsch M. S., Schooley R. T., Rota T. R., Kennedy R. C., Chanh T. C., Sato V. L. Human immunodeficiency virus neutralizing antibodies recognize several conserved domains on the envelope glycoproteins. J Virol. 1987 Jun;61(6):2024–2028. doi: 10.1128/jvi.61.6.2024-2028.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Kodama T., Burns D. P., Silva D. P., Veronese F. D., Desrosiers R. C. Strain-specific neutralizing determinant in the transmembrane protein of simian immunodeficiency virus. J Virol. 1991 Apr;65(4):2010–2018. doi: 10.1128/jvi.65.4.2010-2018.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Marthas M. L., Sutjipto S., Higgins J., Lohman B., Torten J., Luciw P. A., Marx P. A., Pedersen N. C. Immunization with a live, attenuated simian immunodeficiency virus (SIV) prevents early disease but not infection in rhesus macaques challenged with pathogenic SIV. J Virol. 1990 Aug;64(8):3694–3700. doi: 10.1128/jvi.64.8.3694-3700.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. McKeating J. A., McKnight A., McIntosh K., Clapham P. R., Mulder C., Weiss R. A. Evaluation of human and simian immunodeficiency virus plaque and neutralization assays. J Gen Virol. 1989 Dec;70(Pt 12):3327–3333. doi: 10.1099/0022-1317-70-12-3327. [DOI] [PubMed] [Google Scholar]
  12. Murphey-Corb M., Martin L. N., Davison-Fairburn B., Montelaro R. C., Miller M., West M., Ohkawa S., Baskin G. B., Zhang J. Y., Putney S. D. A formalin-inactivated whole SIV vaccine confers protection in macaques. Science. 1989 Dec 8;246(4935):1293–1297. doi: 10.1126/science.2555923. [DOI] [PubMed] [Google Scholar]
  13. Nakane P. K., Kawaoi A. Peroxidase-labeled antibody. A new method of conjugation. J Histochem Cytochem. 1974 Dec;22(12):1084–1091. doi: 10.1177/22.12.1084. [DOI] [PubMed] [Google Scholar]
  14. Nara P. L., Hatch W. C., Dunlop N. M., Robey W. G., Arthur L. O., Gonda M. A., Fischinger P. J. Simple, rapid, quantitative, syncytium-forming microassay for the detection of human immunodeficiency virus neutralizing antibody. AIDS Res Hum Retroviruses. 1987 Fall;3(3):283–302. doi: 10.1089/aid.1987.3.283. [DOI] [PubMed] [Google Scholar]
  15. Rasheed S., Norman G. L., Gill P. S., Meyer P. R., Cheng L., Levine A. M. Virus-neutralizing activity, serologic heterogeneity, and retrovirus isolation from homosexual men in the Los Angeles area. Virology. 1986 Apr 15;150(1):1–9. doi: 10.1016/0042-6822(86)90260-6. [DOI] [PubMed] [Google Scholar]
  16. Salter R. D., Howell D. N., Cresswell P. Genes regulating HLA class I antigen expression in T-B lymphoblast hybrids. Immunogenetics. 1985;21(3):235–246. doi: 10.1007/BF00375376. [DOI] [PubMed] [Google Scholar]
  17. Stromberg K., Benveniste R. E., Arthur L. O., Rabin H., Giddens W. E., Jr, Ochs H. D., Morton W. R., Tsai C. C. Characterization of exogenous type D retrovirus from a fibroma of a macaque with simian AIDS and fibromatosis. Science. 1984 Apr 20;224(4646):289–282. doi: 10.1126/science.6200929. [DOI] [PubMed] [Google Scholar]
  18. Sutjipto S., Kodama T., Yee J., Gettie A., Jennings M., Desrosiers R. C., Marx P. A. Characterization of monoclonal antibodies that distinguish simian immunodeficiency virus isolates from each other and from human immunodeficiency virus types 1 and 2. J Gen Virol. 1990 Jan;71(Pt 1):247–249. doi: 10.1099/0022-1317-71-1-247. [DOI] [PubMed] [Google Scholar]
  19. Sutjipto S., Pedersen N. C., Miller C. J., Gardner M. B., Hanson C. V., Gettie A., Jennings M., Higgins J., Marx P. A. Inactivated simian immunodeficiency virus vaccine failed to protect rhesus macaques from intravenous or genital mucosal infection but delayed disease in intravenously exposed animals. J Virol. 1990 May;64(5):2290–2297. doi: 10.1128/jvi.64.5.2290-2297.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Vujcic L., Katzenstein D., Martin M., Quinnan G. International collaborative study to compare assays for antibodies that neutralize human immunodeficiency virus. AIDS Res Hum Retroviruses. 1990 Jul;6(7):847–853. doi: 10.1089/aid.1990.6.847. [DOI] [PubMed] [Google Scholar]
  21. Weber J., Schild G. World Health Organization workshop on the measurement and significance of neutralizing antibody to HIV and SIV. AIDS. 1989 Feb;3(2):109–111. doi: 10.1097/00002030-198902000-00012. [DOI] [PubMed] [Google Scholar]

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