Abstract
Isolates of Mycobacterium avium serotypes 4 and 8 originating from patients with AIDS in New York City, Los Angeles, or San Francisco were further characterized by multilocus enzyme electrophoresis. Reference strains used to produce typing antisera were also examined. Thirty-one electrophoretic types (ETs) were found among 58 isolates of serotype 4, while 10 ETs were identified among 21 isolates of serotype 8. One major ET was found within each serotype, and these two ETs were closely related, separated by a genetic distance of only 0.05. Six ETs were found in more than one city. In four cases, isolates of serotypes 4 and 8 shared the same ET. Multilocus enzyme electrophoresis in combination with serotyping should be helpful in locating the specific infection sources of these commonly isolated opportunistic pathogens.
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Selected References
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- Abou-Zeid C., Harboe M., Sundsten B., Cocito C. Cross-reactivity of antigens from the cytoplasm and cell walls of some corynebacteria and mycobacteria. J Infect Dis. 1985 Jan;151(1):170–178. doi: 10.1093/infdis/151.1.170. [DOI] [PubMed] [Google Scholar]
- Collins F. M. Mycobacterium avium-complex infections and development of the acquired immunodeficiency syndrome: casual opportunist or causal cofactor? Int J Lepr Other Mycobact Dis. 1986 Sep;54(3):458–474. [PubMed] [Google Scholar]
- Crawford J. T., Bates J. H. Analysis of plasmids in Mycobacterium avium-intracellulare isolates from persons with acquired immunodeficiency syndrome. Am Rev Respir Dis. 1986 Oct;134(4):659–661. doi: 10.1164/arrd.1986.134.4.659. [DOI] [PubMed] [Google Scholar]
- Falkinham J. O., 3rd, Parker B. C., Gruft H. Epidemiology of infection by nontuberculous mycobacteria. I. Geographic distribution in the eastern United States. Am Rev Respir Dis. 1980 Jun;121(6):931–937. doi: 10.1164/arrd.1980.121.6.931. [DOI] [PubMed] [Google Scholar]
- Graham L., Jr, Warren N. G., Tsang A. Y., Dalton H. P. Mycobacterium avium complex pseudobacteriuria from a hospital water supply. J Clin Microbiol. 1988 May;26(5):1034–1036. doi: 10.1128/jcm.26.5.1034-1036.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hampson S. J., Portaels F., Thompson J., Green E. P., Moss M. T., Hermon-Taylor J., McFadden J. J. DNA probes demonstrate a single highly conserved strain of Mycobacterium avium infecting AIDS patients. Lancet. 1989 Jan 14;1(8629):65–68. doi: 10.1016/s0140-6736(89)91427-x. [DOI] [PubMed] [Google Scholar]
- Helbert M., Robinson D., Buchanan D., Hellyer T., McCarthy M., Brown I., Pinching A. J., Mitchell D. M. Mycobacterial infection in patients infected with the human immunodeficiency virus. Thorax. 1990 Jan;45(1):45–48. doi: 10.1136/thx.45.1.45. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Hellyer T. J., Brown I. N., Dale J. W., Easmon C. S. Plasmid analysis of Mycobacterium avium-intracellulare (MAI) isolated in the United Kingdom from patients with and without AIDS. J Med Microbiol. 1991 Apr;34(4):225–231. doi: 10.1099/00222615-34-4-225. [DOI] [PubMed] [Google Scholar]
- Horsburgh C. R., Jr, Cohn D. L., Roberts R. B., Masur H., Miller R. A., Tsang A. Y., Iseman M. D. Mycobacterium avium-M. intracellulare isolates from patients with or without acquired immunodeficiency syndrome. Antimicrob Agents Chemother. 1986 Dec;30(6):955–957. doi: 10.1128/aac.30.6.955. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Jensen A. G., Bennedsen J., Rosdahl V. T. Plasmid profiles of mycobacterium avium/intracellulare isolated from patients with AIDS or cervical lymphadenitis and from environmental samples. Scand J Infect Dis. 1989;21(6):645–649. doi: 10.3109/00365548909021692. [DOI] [PubMed] [Google Scholar]
- Kiehn T. E., Edwards F. F., Brannon P., Tsang A. Y., Maio M., Gold J. W., Whimbey E., Wong B., McClatchy J. K., Armstrong D. Infections caused by Mycobacterium avium complex in immunocompromised patients: diagnosis by blood culture and fecal examination, antimicrobial susceptibility tests, and morphological and seroagglutination characteristics. J Clin Microbiol. 1985 Feb;21(2):168–173. doi: 10.1128/jcm.21.2.168-173.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Meissner G., Anz W. Sources of Mycobacterium avium complex infection resulting in human diseases. Am Rev Respir Dis. 1977 Dec;116(6):1057–1064. doi: 10.1164/arrd.1977.116.6.1057. [DOI] [PubMed] [Google Scholar]
- Saito H., Tomioka H., Sato K., Tasaka H., Dawson D. J. Identification of various serovar strains of Mycobacterium avium complex by using DNA probes specific for Mycobacterium avium and Mycobacterium intracellulare. J Clin Microbiol. 1990 Aug;28(8):1694–1697. doi: 10.1128/jcm.28.8.1694-1697.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saito H., Tomioka H., Sato K., Tasaka H., Tsukamura M., Kuze F., Asano K. Identification and partial characterization of Mycobacterium avium and Mycobacterium intracellulare by using DNA probes. J Clin Microbiol. 1989 May;27(5):994–997. doi: 10.1128/jcm.27.5.994-997.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Selander R. K., Caugant D. A., Ochman H., Musser J. M., Gilmour M. N., Whittam T. S. Methods of multilocus enzyme electrophoresis for bacterial population genetics and systematics. Appl Environ Microbiol. 1986 May;51(5):873–884. doi: 10.1128/aem.51.5.873-884.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Selander R. K., Musser J. M., Caugant D. A., Gilmour M. N., Whittam T. S. Population genetics of pathogenic bacteria. Microb Pathog. 1987 Jul;3(1):1–7. doi: 10.1016/0882-4010(87)90032-5. [DOI] [PubMed] [Google Scholar]
- Stacey A. R. Isolation of Mycobacterium avium-intracellulare-scrofulaceum complex from faeces of patients with AIDS. Br Med J (Clin Res Ed) 1986 Nov 8;293(6556):1194–1194. doi: 10.1136/bmj.293.6556.1194. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wallace R. J., Jr, Musser J. M., Hull S. I., Silcox V. A., Steele L. C., Forrester G. D., Labidi A., Selander R. K. Diversity and sources of rapidly growing mycobacteria associated with infections following cardiac surgery. J Infect Dis. 1989 Apr;159(4):708–716. doi: 10.1093/infdis/159.4.708. [DOI] [PubMed] [Google Scholar]
- Wards B. J., Collins D. M., de Lisle G. W. Restriction endonuclease analysis of members of the Mycobacterium avium-M. intracellulare-M. scrofulaceum serocomplex. J Clin Microbiol. 1987 Dec;25(12):2309–2313. doi: 10.1128/jcm.25.12.2309-2313.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wasem C. F., McCarthy C. M., Murray L. W. Multilocus enzyme electrophoresis analysis of the Mycobacterium avium complex and other mycobacteria. J Clin Microbiol. 1991 Feb;29(2):264–271. doi: 10.1128/jcm.29.2.264-271.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Whipple D. L., Le Febvre R. B., Andrews R. E., Jr, Thiermann A. B. Isolation and analysis of restriction endonuclease digestive patterns of chromosomal DNA from Mycobacterium paratuberculosis and other Mycobacterium species. J Clin Microbiol. 1987 Aug;25(8):1511–1515. doi: 10.1128/jcm.25.8.1511-1515.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Yakrus M. A., Good R. C. Geographic distribution, frequency, and specimen source of Mycobacterium avium complex serotypes isolated from patients with acquired immunodeficiency syndrome. J Clin Microbiol. 1990 May;28(5):926–929. doi: 10.1128/jcm.28.5.926-929.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- du Moulin G. C., Stottmeier K. D., Pelletier P. A., Tsang A. Y., Hedley-Whyte J. Concentration of Mycobacterium avium by hospital hot water systems. JAMA. 1988 Sep 16;260(11):1599–1601. doi: 10.1001/jama.260.11.1599. [DOI] [PubMed] [Google Scholar]