Abstract
A competitive enzyme immunoassay (CEIA) was established and compared with other serological techniques for detecting Coxiella burnetii antibody in camels, goats, and sheep. This technique was evaluated because a conjugated anti-camel immunoglobulin was not available to serve as a direct signal for the demonstration of antigen-antibody reaction. A C. burnetii antibody-positive human serum and a peroxidase-conjugated anti-human immunoglobulin G were used as an indicator system competing against antibody in animal serum or as an indicator of the absence of antibody. Sera were considered antibody positive when the A414 of the test sera plus the competing positive antibody was less than or equal to 50% of the A414 of the negative-control serum plus the competing antibody. Antibody to C. burnetii was repeatedly demonstrated in 66% of camel serum samples (n = 200) by the CEIA. Among 48 camel serum samples, 71% were positive for antibody by CEIA versus 65% by EIA using peroxidase-labeled protein A. The CEIA detected C. burnetii antibody in 63% of sheep serum samples (n = 40) and in 50% of goat serum samples (n = 96), while the indirect fluorescent-antibody technique detected antibody in 38% of sheep and 34% of goat serum samples and the EIA detected antibody in 50% of sheep and 35% of goat serum samples. These data indicate that the CEIA is a reliable and sensitive technique for demonstrating C. burnetii antibody in camels, sheep, and goats.
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Selected References
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- Behymer D. E., Ruppanner R., Brooks D., Williams J. C., Franti C. E. Enzyme immunoassay for surveillance of Q fever. Am J Vet Res. 1985 Nov;46(11):2413–2417. [PubMed] [Google Scholar]
- Biberfield P., Ghetie V., Sjöquist J. Demonstration and assaying of IgG antibodies in tissues and on cells by labeled staphylococcal protein A. J Immunol Methods. 1975 Jan;6(3):249–259. doi: 10.1016/0022-1759(75)90068-x. [DOI] [PubMed] [Google Scholar]
- Enright J. B., Behymer D. E., Franti C. E., Dutson V. J., Longhurst W. M., Wright M. E., Goggin J. E. The behavior of Q fever rickettsiae isolated from wild animals in Northern California. J Wildl Dis. 1971 Apr;7(2):83–90. doi: 10.7589/0090-3558-7.2.83. [DOI] [PubMed] [Google Scholar]
- Forsgren A., Sjöquist J. "Protein A" from Staphylococcus aureus. 3. Reaction with rabbit gamma-globulin. J Immunol. 1967 Jul;99(1):19–24. [PubMed] [Google Scholar]
- Hunt J. G., Field P. R., Murphy A. M. Immunoglobulin responses to Coxiella burnetii (Q fever): single-serum diagnosis of acute infection, using an immunofluorescence technique. Infect Immun. 1983 Feb;39(2):977–981. doi: 10.1128/iai.39.2.977-981.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Kronvall G., Grey H. M., Williams R. C., Jr Protein A reactivity with mouse immunoglobulins. Structural relationship between some mouse and human immunoglobulins. J Immunol. 1970 Nov;105(5):1116–1123. [PubMed] [Google Scholar]
- McDade J. E., Zaklama N. S., Imam I. Z., Wanees M. Serological survey for Q fever in Egyptian domestic animals. J Egypt Public Health Assoc. 1973;48(1-2):101–108. [PubMed] [Google Scholar]
- Orten D. J., Blecha F., Morrill J. L., Minocha H. C. Characterization of anti-idiotype reagents to bovine herpesvirus-1 monoclonal antibody. Vet Immunol Immunopathol. 1988 Dec;20(1):1–14. doi: 10.1016/0165-2427(88)90021-9. [DOI] [PubMed] [Google Scholar]
- Scott T. W., Olson J. G. Detection of eastern equine encephalomyelitis viral antigen in avian blood by enzyme immunoassay: a laboratory study. Am J Trop Med Hyg. 1986 May;35(3):611–618. doi: 10.4269/ajtmh.1986.35.611. [DOI] [PubMed] [Google Scholar]
- Tozawa H., Komatsu H., Ohkata K., Nakajima T., Watanabe M., Tanaka Y., Arifuku M. Neutralizing activity of the antibodies against two kinds of envelope glycoproteins of Sendai virus. Arch Virol. 1986;91(1-2):145–161. doi: 10.1007/BF01316735. [DOI] [PubMed] [Google Scholar]
- Zweygarth E., Sabwa C., Röttcher D. An enzyme-linked immunosorbent assay for the detection of antibodies to Trypanosoma (T.) brucei evansi in camels (Camelus dromedarius) using peroxidase-conjugated protein A. Trop Med Parasitol. 1986 Jun;37(2):105–106. [PubMed] [Google Scholar]
- van Oirschot J. T., Rziha H. J., Moonen P. J., Pol J. M., van Zaane D. Differentiation of serum antibodies from pigs vaccinated or infected with Aujeszky's disease virus by a competitive enzyme immunoassay. J Gen Virol. 1986 Jun;67(Pt 6):1179–1182. doi: 10.1099/0022-1317-67-6-1179. [DOI] [PubMed] [Google Scholar]