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. 1992 Jul;30(7):1703–1710. doi: 10.1128/jcm.30.7.1703-1710.1992

Conserved sequences of the adenovirus genome for detection of all human adenovirus types by hybridization.

T H Scott-Taylor 1, G W Hammond 1
PMCID: PMC265367  PMID: 1629324

Abstract

The application of DNA hybridization directly to clinical specimens has the potential of improving the diagnosis of fastidious types of adenovirus. In this study, the genome of one adenovirus type from each human subgenus (A to F) was systematically evaluated by hybridization for homologous sequences to find the optimal common probe for detection of all human adenovirus types. The area of cross-hybridization, most closely defined with adenovirus type 2 (Ad2), mapped from map units 11.4 to 16.1 and 26.9 to 29.7 and, principally, to a central area of the genome between map units 47.5 and 65.2. The last area, enclosing the hexon gene, was highly conserved. Cloned probes generated from this area demonstrated the greatest homology to heterologous types by hybridization analysis. A HindIII-BglII clone containing the hexon gene of Ad2 within narrow confines reacted most evenly with all adenoviral types and detected the DNA of all other subgenera with a sensitivity 2 logs greater than that of a complete genomic Ad2 probe. The most homologous adenoviral gene sequences were observed in genes involved with DNA replication or intimately connected to the hexon in the early capsid formation. These results show that the hexon gene constitutes the best single region of the adenovirus genome for use as a genus-specific probe for the diagnosis of all human adenoviral subgenera by DNA hybridization.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Adrian T., Wadell G., Hierholzer J. C., Wigand R. DNA restriction analysis of adenovirus prototypes 1 to 41. Arch Virol. 1986;91(3-4):277–290. doi: 10.1007/BF01314287. [DOI] [PubMed] [Google Scholar]
  2. Akusjärvi G., Aleström P., Pettersson M., Lager M., Jörnvall H., Pettersson U. The gene for the adenovirus 2 hexon polypeptide. J Biol Chem. 1984 Nov 25;259(22):13976–13979. [PubMed] [Google Scholar]
  3. Birnboim H. C., Doly J. A rapid alkaline extraction procedure for screening recombinant plasmid DNA. Nucleic Acids Res. 1979 Nov 24;7(6):1513–1523. doi: 10.1093/nar/7.6.1513. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bonner T. I., Brenner D. J., Neufeld B. R., Britten R. J. Reduction in the rate of DNA reassociation by sequence divergence. J Mol Biol. 1973 Dec 5;81(2):123–135. doi: 10.1016/0022-2836(73)90184-8. [DOI] [PubMed] [Google Scholar]
  5. Brandt C. D., Kim H. W., Jeffries B. C., Pyles G., Christmas E. E., Reid J. L., Chanock R. M., Parrott R. H. Infections in 18,000 infants and children in a controlled study of respiratory tract disease. II. Variation in adenovirus infections by year and season. Am J Epidemiol. 1972 Mar;95(3):218–227. doi: 10.1093/oxfordjournals.aje.a121389. [DOI] [PubMed] [Google Scholar]
  6. Cepko C. L., Sharp P. A. Analysis of Ad5 hexon and 100K ts mutants using conformation-specific monoclonal antibodies. Virology. 1983 Aug;129(1):137–154. doi: 10.1016/0042-6822(83)90402-6. [DOI] [PubMed] [Google Scholar]
  7. Chanock R. M. Impact of adenoviruses in human disease. Prev Med. 1974 Dec;3(4):466–472. doi: 10.1016/0091-7435(74)90009-7. [DOI] [PubMed] [Google Scholar]
  8. Collis P. B., Dudding B. A., Winter P. E., Russell P. K., Buescher E. L. Adenovirus vaccines in military recruit populations: a cost-benefit analysis. J Infect Dis. 1973 Dec;128(6):745–752. doi: 10.1093/infdis/128.6.745. [DOI] [PubMed] [Google Scholar]
  9. Everitt E., Lutter L., Philipson L. Structural proteins of adenoviruses. XII. Location and neighbor relationship among proteins of adenovirion type 2 as revealed by enzymatic iodination, immunoprecipitation and chemical cross-linking. Virology. 1975 Sep;67(1):197–208. doi: 10.1016/0042-6822(75)90417-1. [DOI] [PubMed] [Google Scholar]
  10. Fox J. P., Brandt C. D., Wassermann F. E., Hall C. E., Spigland I., Kogon A., Elveback L. R. The virus watch program: a continuing surveillance of viral infections in metropolitan New York families. VI. Observations of adenovirus infections: virus excretion patterns, antibody response, efficiency of surveillance, patterns of infections, and relation to illness. Am J Epidemiol. 1969 Jan;89(1):25–50. doi: 10.1093/oxfordjournals.aje.a120913. [DOI] [PubMed] [Google Scholar]
  11. Friefeld B. R., Lichy J. H., Field J., Gronostajski R. M., Guggenheimer R. A., Krevolin M. D., Nagata K., Hurwitz J., Horwitz M. S. The in vitro replication of adenovirus DNA. Curr Top Microbiol Immunol. 1984;110:221–255. doi: 10.1007/978-3-642-46494-2_8. [DOI] [PubMed] [Google Scholar]
  12. Gary G. W., Jr, Hierholzer J. C., Black R. E. Characteristics of noncultivable adenoviruses associated with diarrhea in infants: a new subgroup of human adenoviruses. J Clin Microbiol. 1979 Jul;10(1):96–103. doi: 10.1128/jcm.10.1.96-103.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Green M., Mackey J. K., Wold W. S., Rigden P. Thirty-one human adenovirus serotypes (Ad1-Ad31) form five groups (A-E) based upon DNA genome homologies. Virology. 1979 Mar;93(2):481–492. doi: 10.1016/0042-6822(79)90251-4. [DOI] [PubMed] [Google Scholar]
  14. Herrmann J. E., Perron-Henry D. M., Stobbs-Walro D., Blacklow N. R. Preparation and characterization of monoclonal antibodies to enteric adenovirus types 40 and 41. Arch Virol. 1987;94(3-4):259–265. doi: 10.1007/BF01310718. [DOI] [PubMed] [Google Scholar]
  15. Kinloch R., Mackay N., Mautner V. Adenovirus hexon. Sequence comparison of subgroup C serotypes 2 and 5. J Biol Chem. 1984 May 25;259(10):6431–6436. [PubMed] [Google Scholar]
  16. Kitchingman G. R. Restriction mapping and molecular cloning of adenovirus type 4 (subgroup E) DNA. Gene. 1982 Dec;20(2):205–210. doi: 10.1016/0378-1119(82)90039-7. [DOI] [PubMed] [Google Scholar]
  17. Larsen S. H., Margolskee R. F., Nathans D. Alignment of the restriction map of mouse adenovirus FL with that of human adenovirus 2. Virology. 1979 Sep;97(2):406–414. doi: 10.1016/0042-6822(79)90351-9. [DOI] [PubMed] [Google Scholar]
  18. Mogabgab W. J. Mycoplasma pneumoniae and adenovirus respiratory illnesses in military and university personnel, 1959-1966. Am Rev Respir Dis. 1968 Mar;97(3):345–358. doi: 10.1164/arrd.1968.97.3.345. [DOI] [PubMed] [Google Scholar]
  19. Persson H., Mathisen B., Philipson L., Pettersson U. A maturation protein in adenovirus morphogenesis. Virology. 1979 Feb;93(1):198–208. doi: 10.1016/0042-6822(79)90287-3. [DOI] [PubMed] [Google Scholar]
  20. Persson H., Philipson L. Regulation of adenovirus gene expression. Curr Top Microbiol Immunol. 1982;97:157–203. doi: 10.1007/978-3-642-68318-3_4. [DOI] [PubMed] [Google Scholar]
  21. Pieniazek N. J., Slemenda S. B., Pieniazek D., Velarde J., Jr, Luftig R. B. Sequence of human enteric adenovirus type 41 Tak fiber protein gene. Nucleic Acids Res. 1989 Nov 25;17(22):9474–9474. doi: 10.1093/nar/17.22.9474. [DOI] [PMC free article] [PubMed] [Google Scholar]
  22. Reed K. C., Mann D. A. Rapid transfer of DNA from agarose gels to nylon membranes. Nucleic Acids Res. 1985 Oct 25;13(20):7207–7221. doi: 10.1093/nar/13.20.7207. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Roberts M. M., White J. L., Grütter M. G., Burnett R. M. Three-dimensional structure of the adenovirus major coat protein hexon. Science. 1986 May 30;232(4754):1148–1151. doi: 10.1126/science.3704642. [DOI] [PubMed] [Google Scholar]
  24. Schildkraut C. Dependence of the melting temperature of DNA on salt concentration. Biopolymers. 1965;3(2):195–208. doi: 10.1002/bip.360030207. [DOI] [PubMed] [Google Scholar]
  25. Scott-Taylor T., Ahluwalia G., Klisko B., Hammond G. W. Prevalent enteric adenovirus variant not detected by commercial monoclonal antibody enzyme immunoassay. J Clin Microbiol. 1990 Dec;28(12):2797–2801. doi: 10.1128/jcm.28.12.2797-2801.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  26. Similä S., Ylikorkala O., Wasz-Höckert O. Type 7 adenovirus pneumonia. J Pediatr. 1971 Oct;79(4):605–611. doi: 10.1016/s0022-3476(71)80307-4. [DOI] [PubMed] [Google Scholar]
  27. Singh-Naz N., Rodriguez W. J., Kidd A. H., Brandt C. D. Monoclonal antibody enzyme-linked immunosorbent assay for specific identification and typing of subgroup F adenoviruses. J Clin Microbiol. 1988 Feb;26(2):297–300. doi: 10.1128/jcm.26.2.297-300.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  28. Slemenda S. B., Pieniazek N. J., Velarde J., Jr, Pieniazek D., Luftig R. B. Nucleotide sequence of the region coding for 100K and 33K proteins of human enteric adenovirus type 41 (Tak). Nucleic Acids Res. 1990 May 25;18(10):3069–3069. doi: 10.1093/nar/18.10.3069. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Takács M., Berencsi G., Lengyel A., Nász I. Restriction site maps of the human adenovirus type 8 DNA. Acta Virol. 1983 Jul;27(4):289–298. [PubMed] [Google Scholar]
  30. Tibbetts C. Physical organization of subgroup B human adenovirus genomes. J Virol. 1977 Nov;24(2):564–579. doi: 10.1128/jvi.24.2.564-579.1977. [DOI] [PMC free article] [PubMed] [Google Scholar]
  31. Tokunaga O., Shinagawa M., Padmanabhan R. Physical mapping of the genome and sequence analysis at the inverted terminal repetition of adenovirus type 4 DNA. Gene. 1982 Jun;18(3):329–334. doi: 10.1016/0378-1119(82)90171-8. [DOI] [PubMed] [Google Scholar]
  32. Toogood C. I., Hay R. T. DNA sequence of the adenovirus type 41 hexon gene and predicted structure of the protein. J Gen Virol. 1988 Sep;69(Pt 9):2291–2301. doi: 10.1099/0022-1317-69-9-2291. [DOI] [PubMed] [Google Scholar]
  33. Toogood C. I., Murali R., Burnett R. M., Hay R. T. The adenovirus type 40 hexon: sequence, predicted structure and relationship to other adenovirus hexons. J Gen Virol. 1989 Dec;70(Pt 12):3203–3214. doi: 10.1099/0022-1317-70-12-3203. [DOI] [PubMed] [Google Scholar]
  34. Uhnoo I., Wadell G., Svensson L., Johansson M. E. Importance of enteric adenoviruses 40 and 41 in acute gastroenteritis in infants and young children. J Clin Microbiol. 1984 Sep;20(3):365–372. doi: 10.1128/jcm.20.3.365-372.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  35. Vos H. L., van der Lee F. M., Reemst A. M., van Loon A. E., Sussenbach J. S. The genes encoding the DNA binding protein and the 23K protease of adenovirus types 40 and 41. Virology. 1988 Mar;163(1):1–10. doi: 10.1016/0042-6822(88)90227-9. [DOI] [PubMed] [Google Scholar]
  36. van Loon A. E., Ligtenberg M., Reemst A. M., Sussenbach J. S., Rozijn T. H. Structure and organization of the left-terminal DNA regions of fastidious adenovirus types 40 and 41. Gene. 1987;58(1):109–126. doi: 10.1016/0378-1119(87)90034-5. [DOI] [PubMed] [Google Scholar]
  37. van Loon A. E., Rozijn T. H., de Jong J. C., Sussenbach J. S. Physicochemical properties of the DNAs of the fastidious adenovirus species 40 and 41. Virology. 1985 Jan 15;140(1):197–200. doi: 10.1016/0042-6822(85)90461-1. [DOI] [PubMed] [Google Scholar]

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