Abstract
Four fluorescein isothiocyanate-labelled monoclonal antibody typing reagents were compared for intensity of fluorescence and sensitivity in confirming herpes simplex virus (HSV) in cell culture. A total of 125 (50 HSV type 1 [HSV-1] and 75 HSV-2) specimens positive for HSV were concurrently stained with type-specific monoclonal antibodies from Bartels (Bartels Immunodiagnostic Supplies, Inc., Bellevue, Wash.), Kallestad (Pathfinder; Kallestad Diagnostics, Chaska, Minn.), Diagnostic Products Corp. (DPC) (PathoDx; DPC, Los Angeles, Calif.), and Syva (Microtrak; Syva Co., Palo Alto, Calif.). Fifty cultured specimens not displaying HSV-associated cytopathic effect were also stained. Each of the four reagents confirmed the 50 negative cultures and 125 positive cultures. One HSV-1-positive culture was missed by the Kallestad antibody. Ten HSV-1 isolates displayed dull fluorescence when stained with the Syva HSV-2 reagent. The other three reagents stained only the HSV-1 well with these 10 specimens. The Syva and Bartels stains were brighter, in general, than the Kallestad and DPC stains. However, the Kallestad and DPC stains were brilliant enough to allow interpretation without hesitation. The differences in intensity and background staining emphasize the need for laboratories to routinely perform quality checks on their reagents and to test new products which become commercially available.
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Selected References
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- Aarnaes S. L., Peterson E. M., de la Maza L. M. Evaluation of a new herpes simplex virus typing reagent for tissue culture confirmation. Diagn Microbiol Infect Dis. 1989 May-Jun;12(3):269–270. doi: 10.1016/0732-8893(89)90026-6. [DOI] [PubMed] [Google Scholar]
- Gleaves C. A., Rice D. H., Bindra R., Hursh D. A., Curtis S. E., Lee C. F., Wendt S. F. Evaluation of a HSV specific monoclonal antibody reagent for laboratory diagnosis of herpes simplex virus infection. Diagn Microbiol Infect Dis. 1989 Jul-Aug;12(4):315–318. doi: 10.1016/0732-8893(89)90096-5. [DOI] [PubMed] [Google Scholar]
- Hsiung G. D. The impact of cell culture sensitivity on rapid viral diagnosis: a historical perspective. Yale J Biol Med. 1989 Mar-Apr;62(2):79–88. [PMC free article] [PubMed] [Google Scholar]
- Johnston S. L., Siegel C. S. Comparison of enzyme immunoassay, shell vial culture, and conventional cell culture for the rapid detection of herpes simplex virus. Diagn Microbiol Infect Dis. 1990 May-Jun;13(3):241–244. doi: 10.1016/0732-8893(90)90066-5. [DOI] [PubMed] [Google Scholar]
- Johnston S. L., Wellens K., Siegel C. S. Rapid isolation of herpes simplex virus by using mink lung and rhabdomyosarcoma cell cultures. J Clin Microbiol. 1990 Dec;28(12):2806–2807. doi: 10.1128/jcm.28.12.2806-2807.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lipson S. M., Salo R. J., Leonardi G. P. Evaluation of five monoclonal antibody-based kits or reagents for the identification and culture confirmation of herpes simplex virus. J Clin Microbiol. 1991 Mar;29(3):466–469. doi: 10.1128/jcm.29.3.466-469.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nahmias A., DelBuono I., Pipkin J., Hutton R., Wickliffe C. Rapid identification and typing of herpes simplex virus types 1 and 2 by a direct immunofluorescence technique. Appl Microbiol. 1971 Sep;22(3):455–458. doi: 10.1128/am.22.3.455-458.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Swierkosz E. M., Arens M. Q., Schmidt R. R., Armstrong T. Evaluation of two immunofluorescence assays with monoclonal antibodies for typing of herpes simplex virus. J Clin Microbiol. 1985 Apr;21(4):643–644. doi: 10.1128/jcm.21.4.643-644.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]