Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Jul;30(7):1891–1892. doi: 10.1128/jcm.30.7.1891-1892.1992

Selective buffered charcoal-yeast extract medium for isolation of nocardiae from mixed cultures.

M A Garrett 1, H T Holmes 1, F S Nolte 1
PMCID: PMC265402  PMID: 1629350

Abstract

The recovery of Nocardia species from mixed cultures is facilitated by use of a selective medium. We show that buffered charcoal-yeast extract medium with polymyxin, anisomycin, and vancomycin can be used for the selective isolation of nocardiae from contaminated specimens.

Full text

PDF
1891

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Mishra S. K., Gordon R. E., Barnett D. A. Identification of nocardiae and streptomycetes of medical importance. J Clin Microbiol. 1980 Jun;11(6):728–736. doi: 10.1128/jcm.11.6.728-736.1980. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Murray P. R., Heeren R. L., Niles A. C. Effect of decontamination procedures on recovery of Nocardia spp. J Clin Microbiol. 1987 Oct;25(10):2010–2011. doi: 10.1128/jcm.25.10.2010-2011.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Murray P. R., Niles A. C., Heeren R. L. Modified Thayer-Martin medium for recovery of Nocardia species from contaminated specimens. J Clin Microbiol. 1988 Jun;26(6):1219–1220. doi: 10.1128/jcm.26.6.1219-1220.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Palmer D. L., Harvey R. L., Wheeler J. K. Diagnostic and therapeutic considerations in Nocardia asteroides infection. Medicine (Baltimore) 1974 Sep;53(5):391–401. doi: 10.1097/00005792-197409000-00005. [DOI] [PubMed] [Google Scholar]
  5. Raad I., Rand K., Gaskins D. Buffered charcoal-yeast extract medium for the isolation of brucellae. J Clin Microbiol. 1990 Jul;28(7):1671–1672. doi: 10.1128/jcm.28.7.1671-1672.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Shawar R. M., Moore D. G., LaRocco M. T. Cultivation of Nocardia spp. on chemically defined media for selective recovery of isolates from clinical specimens. J Clin Microbiol. 1990 Mar;28(3):508–512. doi: 10.1128/jcm.28.3.508-512.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Simpson G. L., Stinson E. B., Egger M. J., Remington J. S. Nocardial infections in the immunocompromised host: A detailed study in a defined population. Rev Infect Dis. 1981 May-Jun;3(3):492–507. doi: 10.1093/clinids/3.3.492. [DOI] [PubMed] [Google Scholar]
  8. Singh M., Sandhu R. S., Randhawa H. S. Comparison of paraffin baiting and conventional culture techniques for isolation of Nocardia asteroides from sputum. J Clin Microbiol. 1987 Jan;25(1):176–177. doi: 10.1128/jcm.25.1.176-177.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Vickers R. M., Rihs J. D., Yu V. L. Clinical demonstration of isolation of Nocardia asteroides on buffered charcoal-yeast extract media. J Clin Microbiol. 1992 Jan;30(1):227–228. doi: 10.1128/jcm.30.1.227-228.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES