Abstract
In a blind comparison, 465 randomly collected clinical fecal specimens were examined. Hemo-De was found to be an excellent replacement for ethyl acetate in the concentration procedure and for carbol-xylene and xylene in the trichrome staining procedure. Elimination of toxic reagents, combined with its lower cost, makes Hemo-De the preferred choice in routine parasitology examinations.
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Selected References
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- Garcia L. S., Shimizu R. Comparison of clinical results for the use of ethyl acetate and diethyl ether in the formalin-ether sedimentation technique performed on polyvinyl alcohol-preserved specimens. J Clin Microbiol. 1981 Apr;13(4):709–713. doi: 10.1128/jcm.13.4.709-713.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Gubash S. M., Milburn L. Use of terpene-based products, with and without anhydrous alcohol, as clearing agents in trichrome staining technique for intestinal protozoa. J Clin Microbiol. 1990 Mar;28(3):610–611. doi: 10.1128/jcm.28.3.610-611.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neimeister R., Logan A. L., Egleton J. H. Modified trichrome staining technique with a xylene substitute. J Clin Microbiol. 1985 Aug;22(2):306–307. doi: 10.1128/jcm.22.2.306-307.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Neimeister R., Logan A. L., Gerber B., Egleton J. H., Kleger B. Hemo-De as substitute for ethyl acetate in formalin-ethyl acetate concentration technique. J Clin Microbiol. 1987 Feb;25(2):425–426. doi: 10.1128/jcm.25.2.425-426.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
- WHEATLEY W. B. A rapid staining procedure for intestinal amoebae and flagellates. Am J Clin Pathol. 1951 Oct;21(10):990–991. doi: 10.1093/ajcp/21.10_ts.990. [DOI] [PubMed] [Google Scholar]
- Young K. H., Bullock S. L., Melvin D. M., Spruill C. L. Ethyl acetate as a substitute for diethyl ether in the formalin-ether sedimentation technique. J Clin Microbiol. 1979 Dec;10(6):852–853. doi: 10.1128/jcm.10.6.852-853.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]