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Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Aug;30(8):2019–2024. doi: 10.1128/jcm.30.8.2019-2024.1992

Standardization of an enzyme immunoassay for human antibody to Haemophilus ducreyi.

M Desjardins 1, C E Thompson 1, L G Filion 1, J O Ndinya-Achola 1, F A Plummer 1, A R Ronald 1, P Piot 1, D W Cameron 1
PMCID: PMC265434  PMID: 1500508

Abstract

We standardized a serologic enzyme immunoassay (EIA) for human immunoglobulin G and M antibodies against Haemophilus ducreyi. We evaluated the performance of this test with respect to the time from acute chancroid and coinfection with human immunodeficiency virus (HIV). Antibody to a crude, soluble bacterial antigen of one H. ducreyi strain was detected in a panel of serum samples from clinically and microbiologically confirmed cases of chancroid and from controls. Test interpretation was standardized for optimal sensitivity and specificity. Performance of the EIA was enhanced in the period of early convalescence from acute primary chancroid and was not diminished in the presence of HIV coinfection. The EIA performed adequately as a serologic screening test for field evaluation and epidemiologic application in conjunction with sexually transmitted disease and HIV detection and control efforts.

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Selected References

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  1. Albritton W. L. Biology of Haemophilus ducreyi. Microbiol Rev. 1989 Dec;53(4):377–389. doi: 10.1128/mr.53.4.377-389.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Cameron D. W., Ngugi E. N., Ronald A. R., Simonsen J. N., Braddick M., Bosire M., Kimata J., Kamala J., Ndinya-Achola J. O., Waiyaki P. G. Condom use prevents genital ulcers in women working as prostitutes. Influence of human immunodeficiency virus infection. Sex Transm Dis. 1991 Jul-Sep;18(3):188–191. doi: 10.1097/00007435-199107000-00013. [DOI] [PubMed] [Google Scholar]
  3. Cameron D. W., Padian N. S. Sexual transmission of HIV and the epidemiology of other sexually transmitted diseases. AIDS. 1990;4 (Suppl 1):S99–103. [PubMed] [Google Scholar]
  4. Cameron D. W., Simonsen J. N., D'Costa L. J., Ronald A. R., Maitha G. M., Gakinya M. N., Cheang M., Ndinya-Achola J. O., Piot P., Brunham R. C. Female to male transmission of human immunodeficiency virus type 1: risk factors for seroconversion in men. Lancet. 1989 Aug 19;2(8660):403–407. doi: 10.1016/s0140-6736(89)90589-8. [DOI] [PubMed] [Google Scholar]
  5. Carne C. A., Weller I. V., Waite J., Briggs M., Pearce F., Adler M. W., Tedder R. S. Impaired responsiveness of homosexual men with HIV antibodies to plasma derived hepatitis B vaccine. Br Med J (Clin Res Ed) 1987 Apr 4;294(6576):866–868. doi: 10.1136/bmj.294.6576.866. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. D'Costa L. J., Bowmer I., Nsanze H., Dylewski J., Fransen L., Plummer F. A., Piot P., Ronald A. R. Advances in the diagnosis and management of chancroid. Sex Transm Dis. 1986 Jul-Sep;13(3 Suppl):189–191. doi: 10.1097/00007435-198607000-00019. [DOI] [PubMed] [Google Scholar]
  7. Des Jarlais D. C., Abdul-Quader A., Minkoff H., Hoegsberg B., Landesman S., Tross S. Crack use and multiple AIDS risk behaviors. J Acquir Immune Defic Syndr. 1991;4(4):446–447. [PubMed] [Google Scholar]
  8. Dylewski J., Nsanze H., Maitha G., Ronald A. Laboratory diagnosis of Haemophilus ducreyi: sensitivity of culture media. Diagn Microbiol Infect Dis. 1986 Mar;4(3):241–245. doi: 10.1016/0732-8893(86)90103-3. [DOI] [PubMed] [Google Scholar]
  9. Fast M. V., D'Costa L. J., Nsanze H., Piot P., Curran J., Karasira P., Mirza N., Maclean I. W., Ronald A. R. The clinical diagnosis of genital ulcer disease in men in the tropics. Sex Transm Dis. 1984 Apr-Jun;11(2):72–76. doi: 10.1097/00007435-198404000-00004. [DOI] [PubMed] [Google Scholar]
  10. Greenblatt R. M., Lukehart S. A., Plummer F. A., Quinn T. C., Critchlow C. W., Ashley R. L., D'Costa L. J., Ndinya-Achola J. O., Corey L., Ronald A. R. Genital ulceration as a risk factor for human immunodeficiency virus infection. AIDS. 1988 Feb;2(1):47–50. doi: 10.1097/00002030-198802000-00008. [DOI] [PubMed] [Google Scholar]
  11. Hammond G. W., Lian C. J., Wilt J. C., Ronald A. R. Comparison of specimen collection and laboratory techniques for isolation of Haemophilus ducreyi. J Clin Microbiol. 1978 Jan;7(1):39–43. doi: 10.1128/jcm.7.1.39-43.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Hanley J. A., McNeil B. J. The meaning and use of the area under a receiver operating characteristic (ROC) curve. Radiology. 1982 Apr;143(1):29–36. doi: 10.1148/radiology.143.1.7063747. [DOI] [PubMed] [Google Scholar]
  13. Hansen E. J., Loftus T. A. Monoclonal antibodies reactive with all strains of Haemophilus ducreyi. Infect Immun. 1984 Apr;44(1):196–198. doi: 10.1128/iai.44.1.196-198.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Huang K. L., Ruben F. L., Rinaldo C. R., Jr, Kingsley L., Lyter D. W., Ho M. Antibody responses after influenza and pneumococcal immunization in HIV-infected homosexual men. JAMA. 1987 Apr 17;257(15):2047–2050. [PubMed] [Google Scholar]
  15. Janoff E. N., Smith P. D., Blaser M. J. Acute antibody responses to Giardia lamblia are depressed in patients with AIDS. J Infect Dis. 1988 Apr;157(4):798–804. doi: 10.1093/infdis/157.4.798. [DOI] [PubMed] [Google Scholar]
  16. Jessamine P. G., Ronald A. R. Chancroid and the role of genital ulcer disease in the spread of human retroviruses. Med Clin North Am. 1990 Nov;74(6):1417–1431. doi: 10.1016/s0025-7125(16)30488-6. [DOI] [PubMed] [Google Scholar]
  17. Karim Q. N., Finn G. Y., Easmon C. S., Dangor Y., Dance D. A., Ngeow Y. F., Ballard R. C. Rapid detection of Haemophilus ducreyi in clinical and experimental infections using monoclonal antibody: a preliminary evaluation. Genitourin Med. 1989 Dec;65(6):361–365. doi: 10.1136/sti.65.6.361. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Metz C. E. Basic principles of ROC analysis. Semin Nucl Med. 1978 Oct;8(4):283–298. doi: 10.1016/s0001-2998(78)80014-2. [DOI] [PubMed] [Google Scholar]
  19. Morse S. A. Chancroid and Haemophilus ducreyi. Clin Microbiol Rev. 1989 Apr;2(2):137–157. doi: 10.1128/cmr.2.2.137. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Museyi K., Van Dyck E., Vervoort T., Taylor D., Hoge C., Piot P. Use of an enzyme immunoassay to detect serum IgG antibodies to Haemophilus ducreyi. J Infect Dis. 1988 May;157(5):1039–1043. doi: 10.1093/infdis/157.5.1039. [DOI] [PubMed] [Google Scholar]
  21. Nettleman M. D. Receiver operator characteristic (ROC) curves. Infect Control Hosp Epidemiol. 1988 Aug;9(8):374–377. doi: 10.1086/645891. [DOI] [PubMed] [Google Scholar]
  22. Ragni M. V., Ruben F. L., Winkelstein A., Spero J. A., Bontempo F. A., Lewis J. H. Antibody responses to immunization of patients with hemophilia with and without evidence of human immunodeficiency virus (human T-lymphotropic virus type III) infection. J Lab Clin Med. 1987 May;109(5):545–549. [PubMed] [Google Scholar]

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