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. 1992 Aug;30(8):2200–2203. doi: 10.1128/jcm.30.8.2200-2203.1992

In vitro antibody secretion by peripheral blood mononuclear cells as an expression of the immune response to Brucella spp. in humans.

J P Vendrell 1, A M Conge 1, M Segondy 1, S Lombroso 1, M F Huguet 1, A Bertrand 1, F Janbon 1, A Serre 1
PMCID: PMC265472  PMID: 1500535

Abstract

We developed an assay to detect antibodies spontaneously secreted in vitro by peripheral blood mononuclear cells (PBMC) against Brucella spp. High levels of anti-Brucella immunoglobulin G (IgG) and/or IgM and/or IgA antibodies were detected in the cell supernatant solution of PBMC cultures for 12 patients suffering from acute or focalized brucellosis and for 5 patients recently vaccinated against brucellosis. This spontaneous in vitro antibody production disappeared 5 to 20 months after onset of clinical signs and 20 to 27 days after vaccination. The transient character of this anti-Brucella antibody production by PBMC is consistent with a temporary in vivo stimulation of the immune system by Brucella antigens. Detection of this secretion could improve the diagnosis of evolutive brucellosis.

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Selected References

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