Abstract
Thirty isolates of Torulopsis glabrata were examined by pulsed-field gel electrophoresis, which resolved 13 DNA pieces, allowing the identification of 12 types. Bands at 1,400, 1,200, 1,070, 1,025, 681, and 500 kbp were conserved. When applied to 18 isolates from an outbreak, 10 distinct types were identified by this technique. Seven patients had isolates which were identical.
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- Burnie J. P., Odds F. C., Lee W., Webster C., Williams J. D. Outbreak of systemic Candida albicans in intensive care unit caused by cross infection. Br Med J (Clin Res Ed) 1985 Mar 9;290(6470):746–748. doi: 10.1136/bmj.290.6470.746. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carle G. F., Olson M. V. An electrophoretic karyotype for yeast. Proc Natl Acad Sci U S A. 1985 Jun;82(11):3756–3760. doi: 10.1073/pnas.82.11.3756. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Carruba G., Pontieri E., De Bernardis F., Martino P., Cassone A. DNA fingerprinting and electrophoretic karyotype of environmental and clinical isolates of Candida parapsilosis. J Clin Microbiol. 1991 May;29(5):916–922. doi: 10.1128/jcm.29.5.916-922.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Doebbeling B. N., Hollis R. J., Isenberg H. D., Wenzel R. P., Pfaller M. A. Restriction fragment analysis of a Candida tropicalis outbreak of sternal wound infections. J Clin Microbiol. 1991 Jun;29(6):1268–1270. doi: 10.1128/jcm.29.6.1268-1270.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Fox B. C., Mobley H. L., Wade J. C. The use of a DNA probe for epidemiological studies of candidiasis in immunocompromised hosts. J Infect Dis. 1989 Mar;159(3):488–494. doi: 10.1093/infdis/159.3.488. [DOI] [PubMed] [Google Scholar]
- Kaufmann C. S., Merz W. G. Electrophoretic karyotypes of Torulopsis glabrata. J Clin Microbiol. 1989 Oct;27(10):2165–2168. doi: 10.1128/jcm.27.10.2165-2168.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lee W., Burnie J. P., Matthews R. C., Oppenheim B. O., Damani N. N. Hospital outbreaks with yeasts. J Hosp Infect. 1991 Jun;18 (Suppl A):237–249. doi: 10.1016/0195-6701(91)90029-8. [DOI] [PubMed] [Google Scholar]
- Lee W., Burnie J., Matthews R. Fingerprinting Candida albicans. J Immunol Methods. 1986 Nov 6;93(2):177–182. doi: 10.1016/0022-1759(86)90186-9. [DOI] [PubMed] [Google Scholar]
- Magee B. B., Koltin Y., Gorman J. A., Magee P. T. Assignment of cloned genes to the seven electrophoretically separated Candida albicans chromosomes. Mol Cell Biol. 1988 Nov;8(11):4721–4726. doi: 10.1128/mcb.8.11.4721. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Matthews R., Burnie J. Assessment of DNA fingerprinting for rapid identification of outbreaks of systemic candidiasis. BMJ. 1989 Feb 11;298(6670):354–357. doi: 10.1136/bmj.298.6670.354. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Reagan D. R., Pfaller M. A., Hollis R. J., Wenzel R. P. Characterization of the sequence of colonization and nosocomial candidemia using DNA fingerprinting and a DNA probe. J Clin Microbiol. 1990 Dec;28(12):2733–2738. doi: 10.1128/jcm.28.12.2733-2738.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rustchenko-Bulgac E. P. Variations of Candida albicans electrophoretic karyotypes. J Bacteriol. 1991 Oct;173(20):6586–6596. doi: 10.1128/jb.173.20.6586-6596.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Schwartz D. C., Cantor C. R. Separation of yeast chromosome-sized DNAs by pulsed field gradient gel electrophoresis. Cell. 1984 May;37(1):67–75. doi: 10.1016/0092-8674(84)90301-5. [DOI] [PubMed] [Google Scholar]
- Vazquez J. A., Beckley A., Sobel J. D., Zervos M. J. Comparison of restriction enzyme analysis and pulsed-field gradient gel electrophoresis as typing systems for Candida albicans. J Clin Microbiol. 1991 May;29(5):962–967. doi: 10.1128/jcm.29.5.962-967.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]