Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1992 Sep;30(9):2495–2497. doi: 10.1128/jcm.30.9.2495-2497.1992

Haemophilus influenzae is frequently detected with monoclonal antibody 8BD9 in sputum samples from patients with cystic fibrosis.

L V Möller 1, G J Ruijs 1, H G Heijerman 1, J Dankert 1, L van Alphen 1
PMCID: PMC265533  PMID: 1401024

Abstract

To determine the frequency of Haemophilus influenzae in sputum from patients with cystic fibrosis (CF), 477 sputum samples obtained from 86 CF patients were analyzed by standard culture and by the in situ immunoperoxidase staining technique with monoclonal antibody 8BD9. H. influenzae was isolated from 109 sputum samples (23%) from 45 patients (52%) and detected by immunoperoxidase staining in 175 sputum samples (37%) obtained from 63 patients (73%). The results of this study demonstrate the frequent presence of H. influenzae in sputum samples from CF patients.

Full text

PDF

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bauernfeind A., Rotter K., Weisslein-Pfister C. Selective procedure to isolate haemophilus influenzae from sputa with large quantities of Pseudomonas aeruginosa. Infection. 1987 Jul-Aug;15(4):278–280. doi: 10.1007/BF01644138. [DOI] [PubMed] [Google Scholar]
  2. Currie D. C., Higgs E., Metcalfe S., Roberts D. E., Cole P. J. Simple method of monitoring colonising microbial load in chronic bronchial sepsis: pilot comparison of reduction in colonising microbial load with antibiotics given intermittently and continuously. J Clin Pathol. 1987 Aug;40(8):830–836. doi: 10.1136/jcp.40.8.830. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Gilligan P. H. Microbiology of airway disease in patients with cystic fibrosis. Clin Microbiol Rev. 1991 Jan;4(1):35–51. doi: 10.1128/cmr.4.1.35. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Groeneveld K., van Alphen L., Geelen-van-den Broek N. J., Eijk P. P., Zanen H. C., van Ketel R. J. Detection of Haemophilus influenzae with monoclonal antibody. Lancet. 1987 Feb 21;1(8530):441–442. doi: 10.1016/s0140-6736(87)90136-x. [DOI] [PubMed] [Google Scholar]
  5. Groeneveld K., van Alphen L., van Ketel R. J., Geelen-van den Broek L., Eijk P. P., Zanen H. C. Nonculture detection of Haemophilus influenzae in sputum with monoclonal antibodies specific for outer membrane lipoprotein P6. J Clin Microbiol. 1989 Oct;27(10):2263–2267. doi: 10.1128/jcm.27.10.2263-2267.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Hoiby N. Epidemiological investigations of the respiratory tract bacteriology in patients with cystic fibrosis. Acta Pathol Microbiol Scand B Microbiol Immunol. 1974 Aug;82(4):541–550. [PubMed] [Google Scholar]
  7. Kilian M. A taxonomic study of the genus Haemophilus, with the proposal of a new species. J Gen Microbiol. 1976 Mar;93(1):9–62. doi: 10.1099/00221287-93-1-9. [DOI] [PubMed] [Google Scholar]
  8. Murray P. R., Washington J. A. Microscopic and baceriologic analysis of expectorated sputum. Mayo Clin Proc. 1975 Jun;50(6):339–344. [PubMed] [Google Scholar]
  9. Pressler T., Szaff M., Høiby N. Antibiotic treatment of Haemophilus influenzae and Haemophilus parainfluenzae infections in patients with cystic fibrosis. Acta Paediatr Scand. 1984 Jul;73(4):541–547. doi: 10.1111/j.1651-2227.1984.tb09968.x. [DOI] [PubMed] [Google Scholar]
  10. Rayner R. J., Hiller E. J., Ispahani P., Baker M. Haemophilus infection in cystic fibrosis. Arch Dis Child. 1990 Mar;65(3):255–258. doi: 10.1136/adc.65.3.255. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Roberts D. E., Cole P. Use of selective media in bacteriological investigation of patients with chronic suppurative respiratory infection. Lancet. 1980 Apr 12;1(8172):796–797. doi: 10.1016/s0140-6736(80)91295-7. [DOI] [PubMed] [Google Scholar]
  12. Wong K., Roberts M. C., Owens L., Fife M., Smith A. L. Selective media for the quantitation of bacteria in cystic fibrosis sputum. J Med Microbiol. 1984 Apr;17(2):113–119. doi: 10.1099/00222615-17-2-113. [DOI] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES