Abstract
Location of the double-bond position of monounsaturated fatty acids of various bacteria was accomplished with combined gas chromatography-mass spectrometry analysis of dimethyl disulfide (DMDS) derivatives. The monoenoic fatty acids from whole cells were converted to methyl esters and then to DMDS adducts and analyzed by capillary gas chromatography-mass spectrometry. The mass spectra of DMDS adducts gave an easily recognizable molecular ion and two major diagnostic ions attributable to fragmentation between the two CH3S groups located at the original site of unsaturation. Twenty-one relatively novel monoenoic fatty acids were identified among the bacteria studied. All Flavobacterium species contained i17:1 omega 8c, Bacillus alvei contained i16:1 omega 11c and i17:1 omega 12c, and Psychrobacter immobilis contained 12:1 omega 9c. Resolution of cis and trans isomers with capillary gas chromatography and subsequent mass spectrometry permitted positive identification of 16:1 omega 7c and 16:1 omega 7t in Arcobacter (Campylobacter) cryaerophila and 16:1 omega 9c and 16:1 omega 9t in Aerococcus viridans.
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- Brenner D. J., Hollis D. G., Moss C. W., English C. K., Hall G. S., Vincent J., Radosevic J., Birkness K. A., Bibb W. F., Quinn F. D. Proposal of Afipia gen. nov., with Afipia felis sp. nov. (formerly the cat scratch disease bacillus), Afipia clevelandensis sp. nov. (formerly the Cleveland Clinic Foundation strain), Afipia broomeae sp. nov., and three unnamed genospecies. J Clin Microbiol. 1991 Nov;29(11):2450–2460. doi: 10.1128/jcm.29.11.2450-2460.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Couderc F., De Briel D., Demont N., Gilard V., Promé J. C. Mass spectrometry as a tool for identifying group D2 corynebacteria by their fatty acid profiles. J Gen Microbiol. 1991 Aug;137(8):1903–1909. doi: 10.1099/00221287-137-8-1903. [DOI] [PubMed] [Google Scholar]
- Moss C. W., Holzer G., Wallace P. L., Hollis D. G. Cellular fatty acid compositions of an unidentified organism and a bacterium associated with cat scratch disease. J Clin Microbiol. 1990 May;28(5):1071–1074. doi: 10.1128/jcm.28.5.1071-1074.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moss C. W., Lambert-Fair M. A. Location of double bonds in monounsaturated fatty acids of Campylobacter cryaerophila with dimethyl disulfide derivatives and combined gas chromatography-mass spectrometry. J Clin Microbiol. 1989 Jul;27(7):1467–1470. doi: 10.1128/jcm.27.7.1467-1470.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Moss C. W., Wallance P. L., Bosley G. S. Identification of monounsaturated fatty acids of Aerococcus viridans with dimethyl disulfide derivatives and combined gas chromatography-mass spectrometry. J Clin Microbiol. 1989 Sep;27(9):2130–2132. doi: 10.1128/jcm.27.9.2130-2132.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nichols P. D., Mayberry W. R., Antworth C. P., White D. C. Determination of monounsaturated double-bond position and geometry in the cellular fatty acids of the pathogenic bacterium Francisella tularensis. J Clin Microbiol. 1985 May;21(5):738–740. doi: 10.1128/jcm.21.5.738-740.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Welch D. F. Applications of cellular fatty acid analysis. Clin Microbiol Rev. 1991 Oct;4(4):422–438. doi: 10.1128/cmr.4.4.422. [DOI] [PMC free article] [PubMed] [Google Scholar]