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. 1993 Jun;31(6):1521–1524. doi: 10.1128/jcm.31.6.1521-1524.1993

Comparison of a whole-virus enzyme immunoassay (EIA) with a peptide-based EIA for detecting rubella virus immunoglobulin G antibodies following rubella vaccination.

M Zrein 1, J H Joncas 1, L Pedneault 1, L Robillard 1, R J Dwyer 1, M Lacroix 1
PMCID: PMC265571  PMID: 8314994

Abstract

A total of 250 human serum samples were tested for rubella virus immunoglobulin G antibodies by two enzyme immunoassays (EIAs), one using whole rubella virus antigen and the other based on the use of synthetic peptide antigen. The samples were taken from 125 volunteers before and after their immunization with the RA 27/3 rubella vaccine. This study indicates that a synthetic peptide-based EIA can favorably replace current viral lysate-based EIAs to detect rubella virus antibodies following immunization. Because the synthetic peptide used in this newly developed EIA represents a putative neutralization epitope of the rubella virus, it could also be instrumental in determining rubella immune status and in assessing vaccine program efficiency.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

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