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. 1993 Jun;31(6):1606–1608. doi: 10.1128/jcm.31.6.1606-1608.1993

Rapid methods for identification of Staphylococcus aureus when both human and animal staphylococci are tested: comparison with a new immunoenzymatic assay.

M C Guardati 1, C A Guzmàn 1, G Piatti 1, C Pruzzo 1
PMCID: PMC265586  PMID: 8315003

Abstract

A new immunoenzymatic assay (IEA) for the identification of Staphylococcus aureus strains of both human and animal origin was compared with rapid commercial kits. The sensitivities and specificities of the commercial kits varied from 90.2 to 96% and 90.8 to 93.7%, respectively. The IEA did not give any false-negative or false-positive results, while commercial kits gave high percentages of false-positive results among clumping factor-positive non-S. aureus strains. The IEA is particularly useful for isolates for which identification is doubtful, for large-scale epidemiological studies, and for identifying isolates from animals as S. aureus.

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Selected References

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  1. Baker J. S., Bormann M. A., Boudreau D. H. Evaluation of various rapid agglutination methods for the identification of Staphylococcus aureus. J Clin Microbiol. 1985 May;21(5):726–729. doi: 10.1128/jcm.21.5.726-729.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Berke A., Tilton R. C. Evaluation of rapid coagulase methods for the identification of Staphylococcus aureus. J Clin Microbiol. 1986 May;23(5):916–919. doi: 10.1128/jcm.23.5.916-919.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Brown W. J. Comparison of a yellow latex reagent with other agglutination methods for the identification of Staphylococcus aureus. J Clin Microbiol. 1986 Mar;23(3):640–642. doi: 10.1128/jcm.23.3.640-642.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Doern G. V. Evaluation of a commercial latex agglutination test for identification of Staphylococcus aureus. J Clin Microbiol. 1982 Mar;15(3):416–418. doi: 10.1128/jcm.15.3.416-418.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Forsgren A. Significance of protein a production by staphylococci. Infect Immun. 1970 Nov;2(5):672–673. doi: 10.1128/iai.2.5.672-673.1970. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Fournier J. M., Boutonnier A., Bouvet A. Staphylococcus aureus strains which are not identified by rapid agglutination methods are of capsular serotype 5. J Clin Microbiol. 1989 Jun;27(6):1372–1374. doi: 10.1128/jcm.27.6.1372-1374.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Gregson D. B., Low D. E., Skulnick M., Simor A. E. Problems with rapid agglutination methods for identification of Staphylococcus aureus when Staphylococcus saprophyticus is being tested. J Clin Microbiol. 1988 Jul;26(7):1398–1399. doi: 10.1128/jcm.26.7.1398-1399.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Guzmàn C. A., Guardati M. C., Fenoglio D., Coratza G., Pruzzo C., Satta G. Novel immunoenzymatic assay for identification of coagulase- and protein A-negative Staphylococcus aureus strains. J Clin Microbiol. 1992 May;30(5):1194–1197. doi: 10.1128/jcm.30.5.1194-1197.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Igimi S., Takahashi E., Mitsuoka T. Staphylococcus schleiferi subsp. coagulans subsp. nov., isolated from the external auditory meatus of dogs with external ear otitis. Int J Syst Bacteriol. 1990 Oct;40(4):409–411. doi: 10.1099/00207713-40-4-409. [DOI] [PubMed] [Google Scholar]
  10. Kronvall G., Dossett J. H., Quie P. G., Williams R. C. Occurrence of protein a in staphylococcal strains: quantitative aspects and correlation to antigenic and bacteriophage types. Infect Immun. 1971 Jan;3(1):10–15. doi: 10.1128/iai.3.1.10-15.1971. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Lairscey R., Buck G. E. Performance of four slide agglutination methods for identification of Staphylococcus aureus when testing methicillin-resistant staphylococci. J Clin Microbiol. 1987 Jan;25(1):181–182. doi: 10.1128/jcm.25.1.181-182.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Larsson A., Sjöquist J. Novel latex agglutination method with chicken anti-protein A for detection of Staphylococcus aureus infections. J Clin Microbiol. 1989 Dec;27(12):2856–2857. doi: 10.1128/jcm.27.12.2856-2857.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. Lindmark R., Movitz J., Sjöquist J. Extracellular protein A from a methicillin-resistant strain of Staphylococcus aureus. Eur J Biochem. 1977 Apr 15;74(3):623–628. doi: 10.1111/j.1432-1033.1977.tb11431.x. [DOI] [PubMed] [Google Scholar]
  14. Maxim P. E., Mathews H. L., Mengoli H. F. Single-tube mixed agglutination test for the detection of staphylococcal protein A. J Clin Microbiol. 1976 Nov;4(5):418–422. doi: 10.1128/jcm.4.5.418-422.1976. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Raus J., Love D. N. Comparison of the staphylocoagulase activities of Staphylococcus aureus and Staphylococcus intermedius on Chromozym-TH. J Clin Microbiol. 1990 Feb;28(2):207–210. doi: 10.1128/jcm.28.2.207-210.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Valisena S., Varaldo P. E., Satta G. Purification and characterization of three separate bacteriolytic enzymes excreted by Staphylococcus aureus, Staphylococcus simulans, and Staphylococcus saprophyticus. J Bacteriol. 1982 Aug;151(2):636–647. doi: 10.1128/jb.151.2.636-647.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Winblad S., Ericson C. Sensitized sheep red cells as a reactant for Staphylococcus aureus protein A. Methodology and epidemiology with special reference to weakly reacting methicillin-resistant strains. Acta Pathol Microbiol Scand B Microbiol Immunol. 1973 Feb;81(1):150–156. [PubMed] [Google Scholar]

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