Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1993 Jun;31(6):1659–1662. doi: 10.1128/jcm.31.6.1659-1662.1993

Proliferation of rat Pneumocystis carinii on cells sheeted on microcarrier beads in spinner flasks.

C H Lee 1, N L Bauer 1, M M Shaw 1, M M Durkin 1, M S Bartlett 1, S F Queener 1, J W Smith 1
PMCID: PMC265602  PMID: 8315014

Abstract

A method of growing rat Pneumocystis carinii with human embryonic lung fibroblasts (HEL-299 cells) sheeted onto microcarrier beads has been developed. This method allows production of large quantities of P. carinii organisms with very little contamination of host cells. A fivefold increase in the numbers of organisms was achieved, as determined by organism count, antigen detection, and DNA quantification. The majority of organisms produced by this method are trophozoites.

Full text

PDF
1659

Images in this article

1660Image
on p.1660
1661Image
on p.1661

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Bartlett M. S., Eichholtz R., Smith J. W. Antimicrobial susceptibility of Pneumocystis carinii in culture. Diagn Microbiol Infect Dis. 1985 Sep;3(5):381–387. doi: 10.1016/0732-8893(85)90076-8. [DOI] [PubMed] [Google Scholar]
  2. Bartlett M. S., Fishman J. A., Queener S. F., Durkin M. M., Jay M. A., Smith J. W. New rat model of Pneumocystis carinii infection. J Clin Microbiol. 1988 Jun;26(6):1100–1102. doi: 10.1128/jcm.26.6.1100-1102.1988. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bartlett M. S., Verbanac P. A., Smith J. W. Cultivation of Pneumocystis carinii with WI-38 cells. J Clin Microbiol. 1979 Dec;10(6):796–799. doi: 10.1128/jcm.10.6.796-799.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cushion M. T., Ebbets D. Growth and metabolism of Pneumocystis carinii in axenic culture. J Clin Microbiol. 1990 Jun;28(6):1385–1394. doi: 10.1128/jcm.28.6.1385-1394.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Cushion M. T., Ruffolo J. J., Linke M. J., Walzer P. D. Pneumocystis carinii: growth variables and estimates in the A549 and WI-38 VA13 human cell lines. Exp Parasitol. 1985 Aug;60(1):43–54. doi: 10.1016/s0014-4894(85)80021-7. [DOI] [PubMed] [Google Scholar]
  6. Cushion M. T., Walzer P. D. Growth and serial passage of Pneumocystis carinii in the A549 cell line. Infect Immun. 1984 May;44(2):245–251. doi: 10.1128/iai.44.2.245-251.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Durkin M. M., Bartlett M. S., Queener S. F., Shaw M. M., Lee C. H., Smith J. W. An enzyme-linked immunosorbent assay for enumeration of Pneumocystis carinii in vitro and in vivo. J Clin Microbiol. 1992 Dec;30(12):3258–3262. doi: 10.1128/jcm.30.12.3258-3262.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Edlind T. D., Bartlett M. S., Weinberg G. A., Prah G. N., Smith J. W. The beta-tubulin gene from rat and human isolates of Pneumocystis carinii. Mol Microbiol. 1992 Nov;6(22):3365–3373. doi: 10.1111/j.1365-2958.1992.tb02204.x. [DOI] [PubMed] [Google Scholar]
  9. Paulsrud J. R., Queener S. F., Bartlett M. S., Smith J. W. Isolation and characterization of rat lung Pneumocystis carinii gp120. J Protozool. 1991 Nov-Dec;38(6):10S–11S. [PubMed] [Google Scholar]
  10. Pifer L. L., Hughes W. T., Murphy M. J., Jr Propagation of Pneumocystis carinii in vitro. Pediatr Res. 1977 Apr;11(4):305–316. doi: 10.1203/00006450-197704000-00010. [DOI] [PubMed] [Google Scholar]
  11. Pifer L. L., Woods D., Hughes W. T. Propagation of Pneumocystis carinii in Vero cell culture. Infect Immun. 1978 Apr;20(1):66–68. doi: 10.1128/iai.20.1.66-68.1978. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Weinberg G. A., Bartlett M. S. Comparison of pulsed field gel electrophoresis karyotypes of Pneumocystis carinii derived from rat lung, cell culture, and ferret lung. J Protozool. 1991 Nov-Dec;38(6):64S–65S. [PubMed] [Google Scholar]
  13. Ypma-Wong M. F., Fonzi W. A., Sypherd P. S. Fungus-specific translation elongation factor 3 gene present in Pneumocystis carinii. Infect Immun. 1992 Oct;60(10):4140–4145. doi: 10.1128/iai.60.10.4140-4145.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES