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. 1993 Jun;31(6):1672–1674. doi: 10.1128/jcm.31.6.1672-1674.1993

Differentiation of Yersinia enterocolitica serotype O:5,27 strains by phenotypic and molecular techniques.

H Fukushima 1, M Gomyoda 1, S Aleksic 1, M Tsubokura 1
PMCID: PMC265606  PMID: 8391027

Abstract

Restriction endonuclease analyses of virulence plasmid DNA (REAP) and chromosomal DNA and other phenotypic characteristics were used to study the differentiation of Yersinia enterocolitica serotype O:5,27 strains. There was a close correlation between REAP patterns and the geographical distribution of serotype O:5,27. Human isolates produced only one REAP pattern, which was also found with isolates from pigs and dogs.

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Selected References

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  1. Aleksić S., Bockemühl J., Wuthe H. H., Aleksić V. Occurrence and clinical importance of the pathogenic serogroup O: 5, 27 of Yersinia enterocolitica in the Federal Republic of Germany and methods for its serological and bacteriological identification. Zentralbl Bakteriol Mikrobiol Hyg A. 1988 Aug;269(2):197–204. doi: 10.1016/s0176-6724(88)80096-8. [DOI] [PubMed] [Google Scholar]
  2. Asakawa Y., Akahane S., Shiozawa K., Honma T. Investigations of source and route of Yersinia enterocolitica infection. Contrib Microbiol Immunol. 1979;5:115–121. [PubMed] [Google Scholar]
  3. Fukushima H., Tsubokura M. Yersinia enterocolitica biotype 3B serotype 03 phage type II infection in pigs. Nihon Juigaku Zasshi. 1985 Dec;47(6):1011–1015. doi: 10.1292/jvms1939.47.1011. [DOI] [PubMed] [Google Scholar]
  4. HIGUCHI K., SMITH J. L. Studies on the nutrition and physiology of Pasteurella pestis. VI. A differential plating medium for the estimation of the mutation rate to avirulence. J Bacteriol. 1961 Apr;81:605–608. doi: 10.1128/jb.81.4.605-608.1961. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Kado C. I., Liu S. T. Rapid procedure for detection and isolation of large and small plasmids. J Bacteriol. 1981 Mar;145(3):1365–1373. doi: 10.1128/jb.145.3.1365-1373.1981. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Kapperud G., Nesbakken T., Aleksic S., Mollaret H. H. Comparison of restriction endonuclease analysis and phenotypic typing methods for differentiation of Yersinia enterocolitica isolates. J Clin Microbiol. 1990 Jun;28(6):1125–1131. doi: 10.1128/jcm.28.6.1125-1131.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Nesbakken T., Kapperud G., Sørum H., Dommarsnes K. Structural variability of 40-50 Mdal virulence plasmids from Yersinia enterocolitica. Geographical and ecological distribution of plasmid variants. Acta Pathol Microbiol Immunol Scand B. 1987 Jun;95(3):167–173. doi: 10.1111/j.1699-0463.1987.tb03107.x. [DOI] [PubMed] [Google Scholar]
  8. Owen R. J., Borman P. A rapid biochemical method for purifying high molecular weight bacterial chromosomal DNA for restriction enzyme analysis. Nucleic Acids Res. 1987 Apr 24;15(8):3631–3631. doi: 10.1093/nar/15.8.3631. [DOI] [PMC free article] [PubMed] [Google Scholar]

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