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. 1993 Jul;31(7):1866–1869. doi: 10.1128/jcm.31.7.1866-1869.1993

Detection of Toxoplasma gondii in venous blood from AIDS patients by polymerase chain reaction.

J Dupouy-Camet 1, S L de Souza 1, C Maslo 1, A Paugam 1, A G Saimot 1, R Benarous 1, C Tourte-Schaefer 1, F Derouin 1
PMCID: PMC265647  PMID: 8349765

Abstract

Detection of Toxoplasma gondii in blood by means of the polymerase chain reaction (PCR) may facilitate the diagnosis and follow-up of cerebral toxoplasmosis in patients with AIDS. We evaluated this approach with seven patients with tissue culture-proven parasitemia, 14 patients with presumptive cerebral toxoplasmosis, and 17 healthy human immunodeficiency virus-positive controls. Each sample of blood was assayed on three different occasions by a PCR assay based on detection of the gene encoding the P30 surface protein. A positive PCR diagnosis required positivity in at least two of the three PCR tests. None of the controls had a positive PCR diagnosis, but six of the seven patients with parasitemia did. Cerebral toxoplasmosis was confirmed in 13 of the 14 patients with a presumptive diagnosis; diagnosis by PCR was positive before treatment for 9 of these 13 patients, whereas tissue culture was positive for only 1 patient. During treatment, blood samples were taken from 14 patients at regular intervals until day 12. PCR diagnosis became negative on ethidium-stained gels, but persistent signals were observed after hybridization, in some cases, for up to 12 days after initiation of therapy. PCR on venous blood could thus be a sensitive and noninvasive method for the diagnosis of cerebral and disseminated toxoplasmosis in AIDS patients and could be a potential tool for monitoring the effects of treatment.

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Selected References

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  1. Bougnoux M. E., Nicaise P., Heyer F., Ancelle T., Pinquier J. L., Dupouy-Camet J., Tourte-Schaeffer C. Diagnostic de la toxoplasmose cérébrale chez les sidéens. Valeur de la recherche d'anticorps dans le liquide céphalo-rachidien. Presse Med. 1990 Nov 17;19(38):1751–1753. [PubMed] [Google Scholar]
  2. Brézin A. P., Eqwuagu C. E., Silveira C., Thulliez P., Martins M. C., Mahdi R. M., Belfort R., Jr, Nussenblatt R. B. Analysis of aqueous humor in ocular toxoplasmosis. N Engl J Med. 1991 Mar 7;324(10):699–699. [PubMed] [Google Scholar]
  3. Burg J. L., Perelman D., Kasper L. H., Ware P. L., Boothroyd J. C. Molecular analysis of the gene encoding the major surface antigen of Toxoplasma gondii. J Immunol. 1988 Nov 15;141(10):3584–3591. [PubMed] [Google Scholar]
  4. Cazenave J., Forestier F., Bessieres M. H., Broussin B., Begueret J. Contribution of a new PCR assay to the prenatal diagnosis of congenital toxoplasmosis. Prenat Diagn. 1992 Feb;12(2):119–127. doi: 10.1002/pd.1970120207. [DOI] [PubMed] [Google Scholar]
  5. Cristina N., Derouin F., Pelloux H., Pierce R., Cesbron-Delauwn M. F., Ambroise-Thomas P. Détection de Toxoplasma gondii chez des patients sidéens par la technique de "Polymerase Chain Reaction" (PCR), à l'aide de la séquence répétée TGR1E. Pathol Biol (Paris) 1992 Jan;40(1):52–55. [PubMed] [Google Scholar]
  6. Dannemann B., McCutchan J. A., Israelski D., Antoniskis D., Leport C., Luft B., Nussbaum J., Clumeck N., Morlat P., Chiu J. Treatment of toxoplasmic encephalitis in patients with AIDS. A randomized trial comparing pyrimethamine plus clindamycin to pyrimethamine plus sulfadiazine. The California Collaborative Treatment Group. Ann Intern Med. 1992 Jan 1;116(1):33–43. doi: 10.7326/0003-4819-116-1-33. [DOI] [PubMed] [Google Scholar]
  7. Derouin F., Garin Y. J. Isolement de Toxoplasma gondii par culture cellulaire chez les sujets infectés par le VIH. Presse Med. 1992 Nov 21;21(39):1853–1856. [PubMed] [Google Scholar]
  8. Derouin F., Mazeron M. C., Garin Y. J. Comparative study of tissue culture and mouse inoculation methods for demonstration of Toxoplasma gondii. J Clin Microbiol. 1987 Sep;25(9):1597–1600. doi: 10.1128/jcm.25.9.1597-1600.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Derouin F., Sarfati C., Beauvais B., Iliou M. C., Dehen L., Lariviere M. Laboratory diagnosis of pulmonary toxoplasmosis in patients with acquired immunodeficiency syndrome. J Clin Microbiol. 1989 Jul;27(7):1661–1663. doi: 10.1128/jcm.27.7.1661-1663.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. Derouin F., Thulliez P., Garin Y. J. Intérêt et limites de la sérologie de toxoplasmose chez les sujets VIH+. Pathol Biol (Paris) 1991 Apr;39(4):255–259. [PubMed] [Google Scholar]
  11. Dupouy-Camet J., Bougnoux M. E., Lavareda de Souza S., Thulliez P., Dommergues M., Mandelbrot L., Ancelle T., Tourte-Schaefer C., Benarous R. Comparative value of polymerase chain reaction and conventional biological tests for the prenatal diagnosis of congenital toxoplasmosis. Ann Biol Clin (Paris) 1992;50(5):315–319. [PubMed] [Google Scholar]
  12. Gross U., Roggenkamp A., Janitschke K., Heesemann J. Improved sensitivity of the polymerase chain reaction for detection of Toxoplasma gondii in biological and human clinical specimens. Eur J Clin Microbiol Infect Dis. 1992 Jan;11(1):33–39. doi: 10.1007/BF01971268. [DOI] [PubMed] [Google Scholar]
  13. Grover C. M., Thulliez P., Remington J. S., Boothroyd J. C. Rapid prenatal diagnosis of congenital Toxoplasma infection by using polymerase chain reaction and amniotic fluid. J Clin Microbiol. 1990 Oct;28(10):2297–2301. doi: 10.1128/jcm.28.10.2297-2301.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Herrmann B. G., Frischauf A. M. Isolation of genomic DNA. Methods Enzymol. 1987;152:180–183. doi: 10.1016/0076-6879(87)52018-3. [DOI] [PubMed] [Google Scholar]
  15. Hitt J. A., Filice G. A. Detection of Toxoplasma gondii parasitemia by gene amplification, cell culture, and mouse inoculation. J Clin Microbiol. 1992 Dec;30(12):3181–3184. doi: 10.1128/jcm.30.12.3181-3184.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Ho-Yen D. O., Joss A. W., Balfour A. H., Smyth E. T., Baird D., Chatterton J. M. Use of the polymerase chain reaction to detect Toxoplasma gondii in human blood samples. J Clin Pathol. 1992 Oct;45(10):910–913. doi: 10.1136/jcp.45.10.910. [DOI] [PMC free article] [PubMed] [Google Scholar]
  17. Holliman R. E., Johnson J. D., Savva D. Diagnosis of cerebral toxoplasmosis in association with AIDS using the polymerase chain reaction. Scand J Infect Dis. 1990;22(2):243–244. doi: 10.3109/00365549009037911. [DOI] [PubMed] [Google Scholar]
  18. Joss A. W., Chatterton J. M., Evans R., Ho-Yen D. O. Toxoplasma polymerase chain reaction on experimental blood samples. J Med Microbiol. 1993 Jan;38(1):38–43. doi: 10.1099/00222615-38-1-38. [DOI] [PubMed] [Google Scholar]
  19. Lebech M., Lebech A. M., Nelsing S., Vuust J., Mathiesen L., Petersen E. Detection of Toxoplasma gondii DNA by polymerase chain reaction in cerebrospinal fluid from AIDS patients with cerebral toxoplasmosis. J Infect Dis. 1992 May;165(5):982–983. doi: 10.1093/infdis/165.5.982. [DOI] [PubMed] [Google Scholar]
  20. Luft B. J., Remington J. S. Toxoplasmic encephalitis in AIDS. Clin Infect Dis. 1992 Aug;15(2):211–222. doi: 10.1093/clinids/15.2.211. [DOI] [PubMed] [Google Scholar]
  21. Nakao M., Konishi E. Proliferation of Toxoplasma gondii in human neutrophils in vitro. Parasitology. 1991 Aug;103(Pt 1):23–27. doi: 10.1017/s0031182000059242. [DOI] [PubMed] [Google Scholar]
  22. Noordhoek G. T., Wolters E. C., de Jonge M. E., van Embden J. D. Detection by polymerase chain reaction of Treponema pallidum DNA in cerebrospinal fluid from neurosyphilis patients before and after antibiotic treatment. J Clin Microbiol. 1991 Sep;29(9):1976–1984. doi: 10.1128/jcm.29.9.1976-1984.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
  23. Parmley S. F., Goebel F. D., Remington J. S. Detection of Toxoplasma gondii in cerebrospinal fluid from AIDS patients by polymerase chain reaction. J Clin Microbiol. 1992 Nov;30(11):3000–3002. doi: 10.1128/jcm.30.11.3000-3002.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  24. Roth A., Roth B., Höffken G., Steuber S., Khalifa K. I., Janitschke K. Application of the polymerase chain reaction in the diagnosis of pulmonary toxoplasmosis in immunocompromised patients. Eur J Clin Microbiol Infect Dis. 1992 Dec;11(12):1177–1181. doi: 10.1007/BF01961141. [DOI] [PubMed] [Google Scholar]
  25. Sugitani M., Inchauspé G., Shindo M., Prince A. M. Sensitivity of serological assays to identify blood donors with hepatitis C viraemia. Lancet. 1992 Apr 25;339(8800):1018–1019. doi: 10.1016/0140-6736(92)90538-e. [DOI] [PubMed] [Google Scholar]
  26. Tirard V., Niel G., Rosenheim M., Katlama C., Ciceron L., Ogunkolade W., Danis M., Gentilini M. Diagnosis of toxoplasmosis in patients with AIDS by isolation of the parasite from the blood. N Engl J Med. 1991 Feb 28;324(9):634–634. doi: 10.1056/nejm199102283240914. [DOI] [PubMed] [Google Scholar]

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