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. 1993 Jul;31(7):1899–1902. doi: 10.1128/jcm.31.7.1899-1902.1993

Total cellular fatty acid composition of cultured Pneumocystis carinii.

J R Paulsrud 1, S F Queener 1, M S Bartlett 1, J W Smith 1
PMCID: PMC265654  PMID: 8349771

Abstract

Oleic acid makes up > 50% of the total fatty acids of Pneumocystis carinii grown on WI-38 cells. Oleic acid levels increased in parallel with increasing trophozoites over 7 days in culture. The fatty acid composition of P. carinii resembles that of certain fungi but differs from those of lung surfactant lipid, host cells, and fetal bovine serum.

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Selected References

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  1. Bartlett M. S., Eichholtz R., Smith J. W. Antimicrobial susceptibility of Pneumocystis carinii in culture. Diagn Microbiol Infect Dis. 1985 Sep;3(5):381–387. doi: 10.1016/0732-8893(85)90076-8. [DOI] [PubMed] [Google Scholar]
  2. Bartlett M. S., Marr J. J., Queener S. F., Klein R. S., Smith J. W. Activity of inosine analogs against Pneumocystis carinii in culture. Antimicrob Agents Chemother. 1986 Jul;30(1):181–183. doi: 10.1128/aac.30.1.181. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Bartlett M. S., Queener S. F., Tidwell R. R., Milhous W. K., Berman J. D., Ellis W. Y., Smith J. W. 8-Aminoquinolines from Walter Reed Army Institute for Research for treatment and prophylaxis of Pneumocystis pneumonia in rat models. Antimicrob Agents Chemother. 1991 Feb;35(2):277–282. doi: 10.1128/aac.35.2.277. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Bartlett M. S., Verbanac P. A., Smith J. W. Cultivation of Pneumocystis carinii with WI-38 cells. J Clin Microbiol. 1979 Dec;10(6):796–799. doi: 10.1128/jcm.10.6.796-799.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Brondz I., Olsen I. Multivariate analyses of cellular carbohydrates and fatty acids of Candida albicans, Torulopsis glabrata, and Saccharomyces cerevisiae. J Clin Microbiol. 1990 Aug;28(8):1854–1857. doi: 10.1128/jcm.28.8.1854-1857.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Delplanque B., Jacotot B. Influence of environmental medium on fatty acid composition of human cells: leukocytes and fibroblasts. Lipids. 1987 Apr;22(4):241–249. doi: 10.1007/BF02533986. [DOI] [PubMed] [Google Scholar]
  7. Edman J. C., Kovacs J. A., Masur H., Santi D. V., Elwood H. J., Sogin M. L. Ribosomal RNA sequence shows Pneumocystis carinii to be a member of the fungi. Nature. 1988 Aug 11;334(6182):519–522. doi: 10.1038/334519a0. [DOI] [PubMed] [Google Scholar]
  8. Edman U., Edman J. C., Lundgren B., Santi D. V. Isolation and expression of the Pneumocystis carinii thymidylate synthase gene. Proc Natl Acad Sci U S A. 1989 Sep;86(17):6503–6507. doi: 10.1073/pnas.86.17.6503. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Isseroff R. R., Martinez D. T., Ziboh V. A. Alterations in fatty acid composition of murine keratinocytes with in vitro cultivation. J Invest Dermatol. 1985 Aug;85(2):131–134. doi: 10.1111/1523-1747.ep12276536. [DOI] [PubMed] [Google Scholar]
  10. Jarnum S., Rasmussen E. F., Ohlsen A. S., Sorensen A. W. Generalized Pneumocystis carinii infection with severe idiopathic hypoproteinemia. Ann Intern Med. 1968 Jan;68(1):138–145. doi: 10.7326/0003-4819-68-1-138. [DOI] [PubMed] [Google Scholar]
  11. Kaneshiro E. S., Cushion M. T., Walzer P. D., Jayasimhulu K. Analyses of Pneumocystis fatty acids. J Protozool. 1989 Jan-Feb;36(1):69S–72S. [PubMed] [Google Scholar]
  12. Koukou A. I., Tsoukatos D., Drainas C. Effect of ethanol on the phospholipid and fatty acid content of Schizosaccharomyces pombe membranes. J Gen Microbiol. 1990 Jul;136(7):1271–1277. doi: 10.1099/00221287-136-7-1271. [DOI] [PubMed] [Google Scholar]
  13. Lepage G., Roy C. C. Improved recovery of fatty acid through direct transesterification without prior extraction or purification. J Lipid Res. 1984 Dec 1;25(12):1391–1396. [PubMed] [Google Scholar]
  14. Lundgren B., Kovacs J. A., Nelson N. N., Stock F., Martinez A., Gill V. J. Pneumocystis carinii and specific fungi have a common epitope, identified by a monoclonal antibody. J Clin Microbiol. 1992 Feb;30(2):391–395. doi: 10.1128/jcm.30.2.391-395.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Marumo K., Aoki Y. Discriminant analysis of cellular fatty acids of Candida species, Torulopsis glabrata, and Cryptococcus neoformans determined by gas-liquid chromatography. J Clin Microbiol. 1990 Jul;28(7):1509–1513. doi: 10.1128/jcm.28.7.1509-1513.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  16. Moss C. W. Gas-liquid chromatography as an analytical tool in microbiology. J Chromatogr. 1981 Jan 9;203:337–347. doi: 10.1016/s0021-9673(00)80305-2. [DOI] [PubMed] [Google Scholar]
  17. Moss C. W., Shinoda T., Samuels J. W. Determination of cellular fatty acid compositions of various yeasts by gas-liquid chromatography. J Clin Microbiol. 1982 Dec;16(6):1073–1079. doi: 10.1128/jcm.16.6.1073-1079.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Pesanti E. L. Phospholipid profile of Pneumocystis carinii and its interaction with alveolar type II epithelial cells. Infect Immun. 1987 Mar;55(3):736–741. doi: 10.1128/iai.55.3.736-741.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  19. Queener S. F., Bartlett M. S., Jay M. A., Durkin M. M., Smith J. W. Activity of lipid-soluble inhibitors of dihydrofolate reductase against Pneumocystis carinii in culture and in a rat model of infection. Antimicrob Agents Chemother. 1987 Sep;31(9):1323–1327. doi: 10.1128/aac.31.9.1323. [DOI] [PMC free article] [PubMed] [Google Scholar]
  20. Queener S. F., Bartlett M. S., Richardson J. D., Durkin M. M., Jay M. A., Smith J. W. Activity of clindamycin with primaquine against Pneumocystis carinii in vitro and in vivo. Antimicrob Agents Chemother. 1988 Jun;32(6):807–813. doi: 10.1128/aac.32.6.807. [DOI] [PMC free article] [PubMed] [Google Scholar]
  21. Sanders R. L., Longmore W. J. Phosphatidyglycerol in rat lung. II. Comparison of occurrence, composition, and metabolism in surfactant and residual lung fractions. Biochemistry. 1975 Feb 25;14(4):835–840. doi: 10.1021/bi00675a030. [DOI] [PubMed] [Google Scholar]
  22. Schutgens R. B., Wanders R. J., Nijenhuis A., van den Hoek C. M., Heymans H. S., Schrakamp G., Bleeker-Wagemakers E. M., Delleman J. W., Schram A. W., Tager J. M. Genetic diseases caused by peroxisomal dysfunction. New findings in clinical and biochemical studies. Enzyme. 1987;38(1-4):161–176. doi: 10.1159/000469202. [DOI] [PubMed] [Google Scholar]
  23. Smith J. W., Bartlett M. S., Queener S. F., Durkin M. M., Jay M. A., Hull M. T., Klein R. S., Marr J. J. Pneumocystis carinii pneumonia therapy with 9-deazainosine in rats. Diagn Microbiol Infect Dis. 1987 Jun;7(2):113–118. doi: 10.1016/0732-8893(87)90028-9. [DOI] [PubMed] [Google Scholar]
  24. Stoll L. L., Spector A. A. Changes in serum influence the fatty acid composition of established cell lines. In Vitro. 1984 Sep;20(9):732–738. doi: 10.1007/BF02618879. [DOI] [PubMed] [Google Scholar]
  25. Stringer S. L., Stringer J. R., Blase M. A., Walzer P. D., Cushion M. T. Pneumocystis carinii: sequence from ribosomal RNA implies a close relationship with fungi. Exp Parasitol. 1989 May;68(4):450–461. doi: 10.1016/0014-4894(89)90130-6. [DOI] [PubMed] [Google Scholar]
  26. Sueishi K., Benson B. J. Isolation of a major apolipoprotein of canine and murine pulmonary surfactant. Biochemical and immunochemical characteristics. Biochim Biophys Acta. 1981 Sep 24;665(3):442–453. doi: 10.1016/0005-2760(81)90257-5. [DOI] [PubMed] [Google Scholar]
  27. Viljoen B. C., Kock J. L., Britz T. J. The significance of long-chain fatty acid composition and other phenotypic characteristics in determining relationships among some Pichia and Candida species. J Gen Microbiol. 1988 Jul;134(7):1893–1899. doi: 10.1099/00221287-134-7-1893. [DOI] [PubMed] [Google Scholar]
  28. White G. L., Hawthorne J. N. Phosphatidic acid and phosphatidylinositol metabolism in Schizosaccharomyces pombe. Biochem J. 1970 Apr;117(2):203–213. doi: 10.1042/bj1170203. [DOI] [PMC free article] [PubMed] [Google Scholar]
  29. Zimmerman P. E., Voelker D. R., McCormack F. X., Paulsrud J. R., Martin W. J., 2nd 120-kD surface glycoprotein of Pneumocystis carinii is a ligand for surfactant protein A. J Clin Invest. 1992 Jan;89(1):143–149. doi: 10.1172/JCI115554. [DOI] [PMC free article] [PubMed] [Google Scholar]

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