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. 1993 Jul;31(7):1940–1942. doi: 10.1128/jcm.31.7.1940-1942.1993

Comparison of arbitrarily primed polymerase chain reaction, ribotyping, and monoclonal antibody analysis for subtyping Legionella pneumophila serogroup 1.

P Gomez-Lus 1, B S Fields 1, R F Benson 1, W T Martin 1, S P O'Connor 1, C M Black 1
PMCID: PMC265666  PMID: 8394380

Abstract

Arbitrarily primed polymerase chain reaction (AP-PCR) was used to characterize Legionella pneumophila serogroup 1. Cells from a single colony could be subtyped by AP-PCR within a few hours. The discrimination between strains of L. pneumophila serogroup 1 by AP-PCR was equivalent to that by monoclonal antibody analysis and ribotyping. Four strains representing the monoclonal antibody pattern most frequently associated with outbreaks all yielded unique amplicon patterns by AP-PCR.

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Selected References

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  1. Graves L. M., Swaminathan B., Reeves M. W., Wenger J. Ribosomal DNA fingerprinting of Listeria monocytogenes using a digoxigenin-labeled DNA probe. Eur J Epidemiol. 1991 Jan;7(1):77–82. doi: 10.1007/BF00221345. [DOI] [PubMed] [Google Scholar]
  2. Haertl R., Bandlow G. Subtyping of Legionella pneumophila serogroup 1 isolates by small-fragment restriction endonuclease analysis. Eur J Clin Microbiol Infect Dis. 1991 Aug;10(8):630–635. doi: 10.1007/BF01975814. [DOI] [PubMed] [Google Scholar]
  3. Joly J. R., McKinney R. M., Tobin J. O., Bibb W. F., Watkins I. D., Ramsay D. Development of a standardized subgrouping scheme for Legionella pneumophila serogroup 1 using monoclonal antibodies. J Clin Microbiol. 1986 Apr;23(4):768–771. doi: 10.1128/jcm.23.4.768-771.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Maher W. E., Plouffe J. F., Para M. F. Plasmid profiles of clinical and environmental isolates of Legionella pneumophila serogroup 1. J Clin Microbiol. 1983 Dec;18(6):1422–1423. doi: 10.1128/jcm.18.6.1422-1423.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Reingold A. L., Thomason B. M., Brake B. J., Thacker L., Wilkinson H. W., Kuritsky J. N. Legionella pneumonia in the United States: the distribution of serogroups and species causing human illness. J Infect Dis. 1984 May;149(5):819–819. doi: 10.1093/infdis/149.5.819. [DOI] [PubMed] [Google Scholar]
  6. Sanden G. N., Cassiday P. K., Barbaree J. M. Rapid immunodot technique for identifying Bordetella pertussis. J Clin Microbiol. 1993 Jan;31(1):170–172. doi: 10.1128/jcm.31.1.170-172.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Saunders N. A., Harrison T. G., Haththotuwa A., Kachwalla N., Taylor A. G. A method for typing strains of Legionella pneumophila serogroup 1 by analysis of restriction fragment length polymorphisms. J Med Microbiol. 1990 Jan;31(1):45–55. doi: 10.1099/00222615-31-1-45. [DOI] [PubMed] [Google Scholar]
  8. Schoonmaker D., Heimberger T., Birkhead G. Comparison of ribotyping and restriction enzyme analysis using pulsed-field gel electrophoresis for distinguishing Legionella pneumophila isolates obtained during a nosocomial outbreak. J Clin Microbiol. 1992 Jun;30(6):1491–1498. doi: 10.1128/jcm.30.6.1491-1498.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Struelens M. J., Maes N., Rost F., Deplano A., Jacobs F., Liesnard C., Bornstein N., Grimont F., Lauwers S., McIntyre M. P. Genotypic and phenotypic methods for the investigation of a nosocomial Legionella pneumophila outbreak and efficacy of control measures. J Infect Dis. 1992 Jul;166(1):22–30. doi: 10.1093/infdis/166.1.22. [DOI] [PubMed] [Google Scholar]
  10. Stull T. L., LiPuma J. J., Edlind T. D. A broad-spectrum probe for molecular epidemiology of bacteria: ribosomal RNA. J Infect Dis. 1988 Feb;157(2):280–286. doi: 10.1093/infdis/157.2.280. [DOI] [PubMed] [Google Scholar]
  11. Tompkins L. S., Troup N. J., Woods T., Bibb W., McKinney R. M. Molecular epidemiology of Legionella species by restriction endonuclease and alloenzyme analysis. J Clin Microbiol. 1987 Oct;25(10):1875–1880. doi: 10.1128/jcm.25.10.1875-1880.1987. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Welsh J., McClelland M. Fingerprinting genomes using PCR with arbitrary primers. Nucleic Acids Res. 1990 Dec 25;18(24):7213–7218. doi: 10.1093/nar/18.24.7213. [DOI] [PMC free article] [PubMed] [Google Scholar]
  13. van Ketel R. J., ter Schegget J., Zanen H. C. Molecular epidemiology of Legionella pneumophila serogroup 1. J Clin Microbiol. 1984 Sep;20(3):362–364. doi: 10.1128/jcm.20.3.362-364.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]

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