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. 1993 Jul;31(7):1940–1942. doi: 10.1128/jcm.31.7.1940-1942.1993

Comparison of arbitrarily primed polymerase chain reaction, ribotyping, and monoclonal antibody analysis for subtyping Legionella pneumophila serogroup 1.

P Gomez-Lus 1, B S Fields 1, R F Benson 1, W T Martin 1, S P O'Connor 1, C M Black 1
PMCID: PMC265666  PMID: 8394380

Abstract

Arbitrarily primed polymerase chain reaction (AP-PCR) was used to characterize Legionella pneumophila serogroup 1. Cells from a single colony could be subtyped by AP-PCR within a few hours. The discrimination between strains of L. pneumophila serogroup 1 by AP-PCR was equivalent to that by monoclonal antibody analysis and ribotyping. Four strains representing the monoclonal antibody pattern most frequently associated with outbreaks all yielded unique amplicon patterns by AP-PCR.

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Selected References

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