Abstract
A polymerase chain reaction was used to evaluate the occurrence of human papillomavirus (HPV) DNA in urine samples compared with that in urethra and cervix samples simultaneously collected with brushes. Of 138 presumably healthy military conscripts, 12 (8%) had HPV DNA-positive urethra samples and 8 (5%) had HPV DNA-positive urine samples. Both the urine and urethra cell samples of five men were positive, with identical types found in the paired specimens. Seven had HPV DNA-positive urethra samples only, and three had HPV DNA-positive urine samples only. Five of 7 urethra samples from males and 11 of 12 urethra samples from females, who were among patients consulting a clinic for adolescents, were positive for HPV DNA. Among those patients whose urethras were positive for HPV DNA, the corresponding urine samples of 3 of the 5 men and all the 11 women were also positive, with one or two HPV types being in common within the paired samples. Among female patients referred to a colposcopy clinic, 49% (241 of 489) of the cervical cell samples and 38% (187 of 489) of the urine specimens were found to be HPV DNA positive. Of the patients whose cervixes were positive for HPV DNA, 65% (158 of 241) of the simultaneously collected urine samples were also positive for HPV DNA. On the other hand, 84% (158 of 187) of the patients with HPV DNA in their urine also had HPV DNA in their cervical samples. Although not all individuals with genital HPV infections could be identified as HPV positive by analysis of urine samples, at least in epidemiological surveys in which invasive samples are difficult to obtain, such as from children, analysis of urine could be an alternative means of identifying HPV DNA.
Full text
PDF
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Goldsborough M. D., DiSilvestre D., Temple G. F., Lorincz A. T. Nucleotide sequence of human papillomavirus type 31: a cervical neoplasia-associated virus. Virology. 1989 Jul;171(1):306–311. doi: 10.1016/0042-6822(89)90545-x. [DOI] [PubMed] [Google Scholar]
- Marich J. E., Pontsler A. V., Rice S. M., McGraw K. A., Dubensky T. W. The phylogenetic relationship and complete nucleotide sequence of human papillomavirus type 35. Virology. 1992 Feb;186(2):770–776. doi: 10.1016/0042-6822(92)90045-q. [DOI] [PubMed] [Google Scholar]
- Melchers W. J., Schift R., Stolz E., Lindeman J., Quint W. G. Human papillomavirus detection in urine samples from male patients by the polymerase chain reaction. J Clin Microbiol. 1989 Aug;27(8):1711–1714. doi: 10.1128/jcm.27.8.1711-1714.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Nakazawa A., Inoue M., Fujita M., Tanizawa O., Hakura A. Detection of human papillomavirus type 16 in sexual partners of patients having cervical cancer by polymerase chain reaction. Jpn J Cancer Res. 1991 Nov;82(11):1187–1190. doi: 10.1111/j.1349-7006.1991.tb01777.x. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Päbo S., Gifford J. A., Wilson A. C. Mitochondrial DNA sequences from a 7000-year old brain. Nucleic Acids Res. 1988 Oct 25;16(20):9775–9787. doi: 10.1093/nar/16.20.9775. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rymark P., Forslund O., Hansson B. G., Lindholm K. Genital HPV infection not a local but a regional infection: experience from a female teenage group. Genitourin Med. 1993 Feb;69(1):18–22. doi: 10.1136/sti.69.1.18. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saiki R. K., Scharf S., Faloona F., Mullis K. B., Horn G. T., Erlich H. A., Arnheim N. Enzymatic amplification of beta-globin genomic sequences and restriction site analysis for diagnosis of sickle cell anemia. Science. 1985 Dec 20;230(4732):1350–1354. doi: 10.1126/science.2999980. [DOI] [PubMed] [Google Scholar]
- Syrjänen K. J. Epidemiology of human papillomavirus (HPV) infections and their associations with genital squamous cell cancer. Review article. APMIS. 1989 Nov;97(11):957–970. doi: 10.1111/j.1699-0463.1989.tb00504.x. [DOI] [PubMed] [Google Scholar]
- de Villiers E. M. Heterogeneity of the human papillomavirus group. J Virol. 1989 Nov;63(11):4898–4903. doi: 10.1128/jvi.63.11.4898-4903.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]