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Experimental set up. a, A schematic drawing of a PDMS microfluidic device with a D. melanogaster embryo developing in a temperature-step (T-step). b, A microphotograph illustrating the T-step around the embryo, visualized using a suspension of thermochromic liquid crystals (Image Therm Engineering) flowing at a flow rate of 50 mm s−1. The temperature of the green stream is 21 °C and the temperature of the red stream is 24 °C. Scale bar, 400 µm.
