Figure 1.

Mutation of the YRHHY domain of Vif in the context of replication-competent HIV-1 results in loss of Vif function against A3G but not A3F. HIV WT and pHIV-YRHHY > A5, a replication competent HIV-1 containing the YRHHY > A5 mutation, were transfected into 293T cells in the presence of A3G, A3F, or D128K-A3G (a Vif-resistant mutant of A3G). The infectivity of the virus produced from the transfected cells, harvested after 48 hours, was determined by infection of TZM-bl indicator cells and quantitation of the resulting luciferase enzyme activity. The data shown are plotted as the infectivity relative to that produced in the absence of any APOBEC3 proteins which was set to 100%, with standard deviation from two independent experiments.