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. 1993 Aug;31(8):2185–2191. doi: 10.1128/jcm.31.8.2185-2191.1993

Comparison of the reactivities of baculovirus-expressed recombinant Norwalk virus capsid antigen with those of the native Norwalk virus antigen in serologic assays and some epidemiologic observations.

K Y Green 1, J F Lew 1, X Jiang 1, A Z Kapikian 1, M K Estes 1
PMCID: PMC265719  PMID: 8396590

Abstract

Since the discovery of the Norwalk virus (NV) by immune electron microscopy (IEM) in 1972, serologic studies with this virus have relied on particle-positive fecal material from infected volunteers as the source of antigen because it has not been possible to propagate this virus in cell culture. However, the recent cloning of the NV (strain 8FIIa) genome and expression of the capsid protein in a baculovirus system to form "virus-like particles" has provided a consistent source of antigen (designated rNV). The purpose of the present study was to compare the antigenicities of these rNV particles with those of native NV antigen derived from human fecal material by using well-characterized sera obtained from earlier studies. In IEM studies, the rNV antigen reacted with NV-specific antibodies in a manner similar to that observed previously when particle-positive fecal material was used as antigen. In addition, a direct enzyme-linked immunosorbent assay, in which the rNV antigen was used as antigen, proved efficient and specific for the detection of serologic responses to NV compared with the previously established techniques of IEM and blocking antibody immunoassays in which particle-positive fecal material was used as the antigen. The availability of an unlimited source of antigen will enable serologic studies that will greatly increase our understanding of the epidemiology of NV and its role in human enteric illness.

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Selected References

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  1. Adler J. L., Zickl R. Winter vomiting disease. J Infect Dis. 1969 Jun;119(6):668–673. doi: 10.1093/infdis/119.6.668. [DOI] [PubMed] [Google Scholar]
  2. Buscho R., Wyatt R. G., Dolin R., Blacklow N. R., DuPont H. L., Chanock R. M. Recurrent institutional outbreaks of acute infectious nonbacterial gastroenteritis: epidemiology and etiology. Am J Epidemiol. 1973 Sep;98(3):192–198. doi: 10.1093/oxfordjournals.aje.a121548. [DOI] [PubMed] [Google Scholar]
  3. Clarke S. K., Cook G. T., Egglestone S. I., Hall T. S., Miller D. L., Reed S. E., Rubenstein D., Smith A. J., Tyrrell D. A. A virus from epidemic vomiting disease. Br Med J. 1972 Jul 8;3(5818):86–89. doi: 10.1136/bmj.3.5818.86. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Cukor G., Nowak N. A., Blacklow N. R. Immunoglobulin M responses to the Norwalk virus of gastroenteritis. Infect Immun. 1982 Aug;37(2):463–468. doi: 10.1128/iai.37.2.463-468.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Dolin R., Blacklow N. R., DuPont H., Buscho R. F., Wyatt R. G., Kasel J. A., Hornick R., Chanock R. M. Biological properties of Norwalk agent of acute infectious nonbacterial gastroenteritis. Proc Soc Exp Biol Med. 1972 Jun;140(2):578–583. doi: 10.3181/00379727-140-36508. [DOI] [PubMed] [Google Scholar]
  6. Dolin R., Levy A. G., Wyatt R. G., Thornhill T. S., Gardner J. D. Viral gastroenteritis induced by the Hawaii agent. Jejunal histopathology and serologic response. Am J Med. 1975 Dec;59(6):761–768. doi: 10.1016/0002-9343(75)90461-1. [DOI] [PubMed] [Google Scholar]
  7. Gary G. W., Jr, Kaplan J. E., Stine S. E., Anderson L. J. Detection of Norwalk virus antibodies and antigen with a biotin-avidin immunoassay. J Clin Microbiol. 1985 Aug;22(2):274–278. doi: 10.1128/jcm.22.2.274-278.1985. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. Greenberg H. B., Wyatt R. G., Valdesuso J., Kalica A. R., London W. T., Chanock R. M., Kapikian A. Z. Solid-phase microtiter radioimmunoassay for detection of the Norwalk strain of acute nonbacterial, epidemic gastroenteritis virus and its antibodies. J Med Virol. 1978;2(2):97–108. doi: 10.1002/jmv.1890020204. [DOI] [PubMed] [Google Scholar]
  9. Hyams K. C., Bourgeois A. L., Merrell B. R., Rozmajzl P., Escamilla J., Thornton S. A., Wasserman G. M., Burke A., Echeverria P., Green K. Y. Diarrheal disease during Operation Desert Shield. N Engl J Med. 1991 Nov 14;325(20):1423–1428. doi: 10.1056/NEJM199111143252006. [DOI] [PubMed] [Google Scholar]
  10. Hyams K. C., Malone J. D., Kapikian A. Z., Estes M. K., Xi J., Bourgeois A. L., Paparello S., Hawkins R. E., Green K. Y. Norwalk virus infection among Desert Storm troops. J Infect Dis. 1993 Apr;167(4):986–987. doi: 10.1093/infdis/167.4.986. [DOI] [PubMed] [Google Scholar]
  11. Jiang X., Wang M., Graham D. Y., Estes M. K. Expression, self-assembly, and antigenicity of the Norwalk virus capsid protein. J Virol. 1992 Nov;66(11):6527–6532. doi: 10.1128/jvi.66.11.6527-6532.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Johnson P. C., Mathewson J. J., DuPont H. L., Greenberg H. B. Multiple-challenge study of host susceptibility to Norwalk gastroenteritis in US adults. J Infect Dis. 1990 Jan;161(1):18–21. doi: 10.1093/infdis/161.1.18. [DOI] [PubMed] [Google Scholar]
  13. Kapikian A. Z., Greenberg H. B., Cline W. L., Kalica A. R., Wyatt R. G., James H. D., Jr, Lloyd N. L., Chanock R. M., Ryder R. W., Kim H. W. Prevalence of antibody to the Norwalk agent by a newly developed immune adherence hemagglutination assay. J Med Virol. 1978;2(4):281–294. doi: 10.1002/jmv.1890020402. [DOI] [PubMed] [Google Scholar]
  14. Kapikian A. Z., Wyatt R. G., Dolin R., Thornhill T. S., Kalica A. R., Chanock R. M. Visualization by immune electron microscopy of a 27-nm particle associated with acute infectious nonbacterial gastroenteritis. J Virol. 1972 Nov;10(5):1075–1081. doi: 10.1128/jvi.10.5.1075-1081.1972. [DOI] [PMC free article] [PubMed] [Google Scholar]
  15. Koch M. J., Brody J. A. Letter: Lack of teratogenic effects in a presumed parvovirus epidemic. N Engl J Med. 1974 Jun 20;290(25):1437–1437. doi: 10.1056/nejm197406202902514. [DOI] [PubMed] [Google Scholar]
  16. Parrino T. A., Schreiber D. S., Trier J. S., Kapikian A. Z., Blacklow N. R. Clinical immunity in acute gastroenteritis caused by Norwalk agent. N Engl J Med. 1977 Jul 14;297(2):86–89. doi: 10.1056/NEJM197707142970204. [DOI] [PubMed] [Google Scholar]
  17. Pickering L. K., DuPont H. L., Blacklow N. R., Cukor G. Diarrhea due to Norwalk virus in families. J Infect Dis. 1982 Jul;146(1):116–117. doi: 10.1093/infdis/146.1.116. [DOI] [PubMed] [Google Scholar]
  18. Thornhill T. S., Wyatt R. G., Kalica A. R., Dolin R., Chanock R. M., Kapikian A. Z. Detection by immune electron microscopy of 26- to 27-nm viruslike particles associated with two family outbreaks of gastroenteritis. J Infect Dis. 1977 Jan;135(1):20–27. doi: 10.1093/infdis/135.1.20. [DOI] [PubMed] [Google Scholar]
  19. Vesikari T., Varis T., Green K., Flores J., Kapikian A. Z. Immunogenicity and safety of rhesus-human rotavirus reassortant vaccines with serotype 1 or 2 VP7 specificity. Vaccine. 1991 May;9(5):334–339. doi: 10.1016/0264-410x(91)90060-j. [DOI] [PubMed] [Google Scholar]
  20. Wyatt R. G., Dolin R., Blacklow N. R., DuPont H. L., Buscho R. F., Thornhill T. S., Kapikian A. Z., Chanock R. M. Comparison of three agents of acute infectious nonbacterial gastroenteritis by cross-challenge in volunteers. J Infect Dis. 1974 Jun;129(6):709–714. doi: 10.1093/infdis/129.6.709. [DOI] [PubMed] [Google Scholar]
  21. Wyatt R. G., Greenberg H. B., Dalgard D. W., Allen W. P., Sly D. L., Thornhill T. S., Chanock R. M., Kapikian A. Z. Experimental infection of chimpanzees with the Norwalk agent of epidemic viral gastroenteritis. J Med Virol. 1978;2(2):89–96. doi: 10.1002/jmv.1890020203. [DOI] [PubMed] [Google Scholar]
  22. Wyatt R. G., Zapikian A. Z. Viral agents associated with acute gastroenteritis in humans. Am J Clin Nutr. 1977 Nov;30(11):1857–1870. doi: 10.1093/ajcn/30.11.1857. [DOI] [PubMed] [Google Scholar]
  23. Xi J. N., Graham D. Y., Wang K. N., Estes M. K. Norwalk virus genome cloning and characterization. Science. 1990 Dec 14;250(4987):1580–1583. doi: 10.1126/science.2177224. [DOI] [PubMed] [Google Scholar]

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