Abstract
Well-defined Legionella pneumophila strains were analyzed by amplification of variable genomic regions with arbitrary and repeat sequence primers. Clinical and environmental outbreak-related isolates showed closely related amplicon patterns. Eleven strains of unrelated origins displayed 10 distinct patterns. Fingerprinting of L. pneumophila by polymerase chain reaction appeared to have the potential of being as epidemiologically useful as other genotypic methods.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Deragon J. M., Landry B. S. RAPD and other PCR-based analyses of plant genomes using DNA extracted from small leaf disks. PCR Methods Appl. 1992 Feb;1(3):175–180. doi: 10.1101/gr.1.3.175. [DOI] [PubMed] [Google Scholar]
- Erlich H. A., Gelfand D., Sninsky J. J. Recent advances in the polymerase chain reaction. Science. 1991 Jun 21;252(5013):1643–1651. doi: 10.1126/science.2047872. [DOI] [PubMed] [Google Scholar]
- Jayarao B. M., Bassam B. J., Caetano-Anollés G., Gresshoff P. M., Oliver S. P. Subtyping of Streptococcus uberis by DNA amplification fingerprinting. J Clin Microbiol. 1992 May;30(5):1347–1350. doi: 10.1128/jcm.30.5.1347-1350.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Riley D. E., Samadpour M., Krieger J. N. Detection of variable DNA repeats in diverse eukaryotic microorganisms by a single set of polymerase chain reaction primers. J Clin Microbiol. 1991 Dec;29(12):2746–2751. doi: 10.1128/jcm.29.12.2746-2751.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Struelens M. J., Maes N., Rost F., Deplano A., Jacobs F., Liesnard C., Bornstein N., Grimont F., Lauwers S., McIntyre M. P. Genotypic and phenotypic methods for the investigation of a nosocomial Legionella pneumophila outbreak and efficacy of control measures. J Infect Dis. 1992 Jul;166(1):22–30. doi: 10.1093/infdis/166.1.22. [DOI] [PubMed] [Google Scholar]
- Versalovic J., Koeuth T., Lupski J. R. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 1991 Dec 25;19(24):6823–6831. doi: 10.1093/nar/19.24.6823. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Welsh J., McClelland M. Genomic fingerprinting using arbitrarily primed PCR and a matrix of pairwise combinations of primers. Nucleic Acids Res. 1991 Oct 11;19(19):5275–5279. doi: 10.1093/nar/19.19.5275. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Belkum A., Bax R., Peerbooms P., Goessens W. H., van Leeuwen N., Quint W. G. Comparison of phage typing and DNA fingerprinting by polymerase chain reaction for discrimination of methicillin-resistant Staphylococcus aureus strains. J Clin Microbiol. 1993 Apr;31(4):798–803. doi: 10.1128/jcm.31.4.798-803.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- van Belkum A., De Jonckheere J., Quint W. G. Genotyping Naegleria spp. and Naegleria fowleri isolates by interrepeat polymerase chain reaction. J Clin Microbiol. 1992 Oct;30(10):2595–2598. doi: 10.1128/jcm.30.10.2595-2598.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]