Abstract
Molecular typing and serotyping were compared for 150 Chlamydia trachomatis strains isolated from genital sources, belonging to 10 different serovars. Because of the general agreement of the two methods, molecular omp1 genotyping was applied to the epidemiological study of C. trachomatis isolates from genital infections in Bordeaux (France), during a 29-month period. The most prevalent omp1 genotypes were E (51.7%), F (17.3%), D (8.8%), and G (8.4%). Restriction enzyme analysis allowed identification of a serovar D variant (Dv), whereas serovar E strains were homogeneous.
Full text
PDF
Images in this article
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Frost E. H., Deslandes S., Veilleux S., Bourgaux-Ramoisy D. Typing Chlamydia trachomatis by detection of restriction fragment length polymorphism in the gene encoding the major outer membrane protein. J Infect Dis. 1991 May;163(5):1103–1107. doi: 10.1093/infdis/163.5.1103. [DOI] [PubMed] [Google Scholar]
- Kuo C. C., Wang S. P., Holmes K. K., Grayston J. T. Immunotypes of Chlamydia trachomatis isolates in Seattle, Washington. Infect Immun. 1983 Aug;41(2):865–868. doi: 10.1128/iai.41.2.865-868.1983. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Lampe M. F., Suchland R. J., Stamm W. E. Nucleotide sequence of the variable domains within the major outer membrane protein gene from serovariants of Chlamydia trachomatis. Infect Immun. 1993 Jan;61(1):213–219. doi: 10.1128/iai.61.1.213-219.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Näher H., Petzoldt D. Serovar distribution of urogenital C trachomatis isolates in Germany. Genitourin Med. 1991 Apr;67(2):114–116. doi: 10.1136/sti.67.2.114. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Persson K., Osser S. Serovars of Chlamydia trachomatis causing postabortion salpingitis. Eur J Clin Microbiol Infect Dis. 1989 Sep;8(9):795–798. doi: 10.1007/BF02185848. [DOI] [PubMed] [Google Scholar]
- Peterson E. M., Markoff B. A., de la Maza L. M. The major outer membrane protein nucleotide sequence of Chlamydia trachomatis, serovar E. Nucleic Acids Res. 1990 Jun 11;18(11):3414–3414. doi: 10.1093/nar/18.11.3414. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rodriguez P., Vekris A., de Barbeyrac B., Dutilh B., Bonnet J., Bebear C. Typing of Chlamydia trachomatis by restriction endonuclease analysis of the amplified major outer membrane protein gene. J Clin Microbiol. 1991 Jun;29(6):1132–1136. doi: 10.1128/jcm.29.6.1132-1136.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Saikku P., Wang S. P. Chlamydia trachomatis immunotypes in Finland. Acta Pathol Microbiol Immunol Scand B. 1987 Apr;95(2):131–134. doi: 10.1111/j.1699-0463.1987.tb03100.x. [DOI] [PubMed] [Google Scholar]
- Sayada C., Denamur E., Orfila J., Catalan F., Elion J. Rapid genotyping of the Chlamydia trachomatis major outer membrane protein by the polymerase chain reaction. FEMS Microbiol Lett. 1991 Sep 15;67(1):73–78. doi: 10.1016/0378-1097(91)90447-i. [DOI] [PubMed] [Google Scholar]
- Sayada C., Denamur E., Xerri B., Orfila J., Catalan F., Elion J. Epidémiologie de Chlamydia trachomatis par l'analyse du gène de la protéine majeure de membrane externe. Pathol Biol (Paris) 1992 May;40(5):583–589. [PubMed] [Google Scholar]
- Wagenvoort J. H., Suchland R. J., Stamm W. E. Serovar distribution of urogenital Chlamydia trachomatis strains in The Netherlands. Genitourin Med. 1988 Jun;64(3):159–161. doi: 10.1136/sti.64.3.159. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Wang S. P., Grayston J. T. Three new serovars of Chlamydia trachomatis: Da, Ia, and L2a. J Infect Dis. 1991 Feb;163(2):403–405. doi: 10.1093/infdis/163.2.403. [DOI] [PubMed] [Google Scholar]
- Wang S. P., Kuo C. C., Barnes R. C., Stephens R. S., Grayston J. T. Immunotyping of Chlamydia trachomatis with monoclonal antibodies. J Infect Dis. 1985 Oct;152(4):791–800. doi: 10.1093/infdis/152.4.791. [DOI] [PubMed] [Google Scholar]