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. 1993 Sep;31(9):2309–2314. doi: 10.1128/jcm.31.9.2309-2314.1993

Characterization of the pseudorabies virus-specific immunoglobulin M response and evaluation of its diagnostic use in pigs with preexisting immunity to the virus.

T G Kimman 1
PMCID: PMC265752  PMID: 8408547

Abstract

Despite preexisting immunity to pseudorabies virus (PRV), pigs may become infected and may or may not show clinical signs of disease. To investigate whether detection of immunoglobulin M (IgM) antibodies to PRV is suitable for diagnosis of recent infection in pigs with (or without) preexisting immunity, the IgM responses of pigs were examined after both experimental and natural infections. Upon inoculation of seronegative pigs with a low dose of a mildly virulent strain of PRV, IgM was first detectable at day 7 postinoculation (p.i.), reached a maximum at day 14 p.i., and became undetectable again at about days 32 to 36 p.i. In inoculated pigs with maternal antibodies against PRV, the IgM response began later and ended sooner, and peak titers were also lower. In immune pigs with maternally derived antibodies, there was apparently no correlation between the virulence of the inoculated strain and the IgM response. The suitability of the IgM enzyme-linked immunosorbent assay (ELISA) for detection of recent infection in the field was compared with that of the virus neutralization (VN) assay and with an ELISA which specifically detects antibodies directed to glycoprotein I (gI) of PRV. Paired sera were obtained from pigs suspected of PRV infection in an area endemic for PRV infection in which vaccination against PRV is often applied. Practically all pigs had antibodies to PRV in the acute phase of the disease. Compared with the VN assay, the specificity of the IgM ELISA was high but its sensitivity was low. However, all three serotests apparently failed to detect some PRV infections. The IgM ELISA appeared to be especially useful as a diagnostic aid for detection of recent infections in pigs with high levels of neutralizing and gI antibodies, probably maternally derived, in the acute phase of the disease. Such pigs may fail to develop a significant rise in VN antibody titer. The IgM ELISA may be the only serotest for monitoring infections in such pigs.

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Selected References

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  1. Butler J. E., Heo Y., Adams P., Richerson H. B. The antigen-limited nature of microtiter ELISAs requires partial depletion of IgG to permit reliable determination of rabbit serum IgA antibody activity. Mol Immunol. 1990 Apr;27(4):319–325. doi: 10.1016/0161-5890(90)90044-z. [DOI] [PubMed] [Google Scholar]
  2. Duffy S. J., Morrison R. B., Thawley D. G. Spread of pseudorabies virus among breeding swine in quarantined herds. J Am Vet Med Assoc. 1991 Jul 1;199(1):61–65. [PubMed] [Google Scholar]
  3. James K. Immunoserology of infectious diseases. Clin Microbiol Rev. 1990 Apr;3(2):132–152. doi: 10.1128/cmr.3.2.132. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Kimman T. G., Brouwers R. A., Daus F. J., van Oirschot J. T., van Zaane D. Measurement of isotype-specific antibody responses to Aujeszky's disease virus in sera and mucosal secretions of pigs. Vet Immunol Immunopathol. 1992 Feb 15;31(1-2):95–113. doi: 10.1016/0165-2427(92)90089-9. [DOI] [PubMed] [Google Scholar]
  5. Kimman T. G., Oei-Lie N., van Zaane D. Role of memory B-cell responses in serum and mucosal fluids of swine for protective immunity against pseudorabies virus. Am J Vet Res. 1992 Nov;53(11):1992–1998. [PubMed] [Google Scholar]
  6. Kimman T. G., de Wind N., Oei-Lie N., Pol J. M., Berns A. J., Gielkens A. L. Contribution of single genes within the unique short region of Aujeszky's disease virus (suid herpesvirus type 1) to virulence, pathogenesis and immunogenicity. J Gen Virol. 1992 Feb;73(Pt 2):243–251. doi: 10.1099/0022-1317-73-2-243. [DOI] [PubMed] [Google Scholar]
  7. Kit S., Sheppard M., Ichimura H., Kit M. Second-generation pseudorabies virus vaccine with deletions in thymidine kinase and glycoprotein genes. Am J Vet Res. 1987 May;48(5):780–793. [PubMed] [Google Scholar]
  8. Martin S., Wardley R. C., Donaldson A. I. Serological response of pigs infected with Aujeszky's disease virus. Res Vet Sci. 1983 Sep;35(2):227–233. [PubMed] [Google Scholar]
  9. McCaw M. B., Molitor T. W., Joo H. S. Characterization of pseudorabies virus antibody responses in young swine after infection and vaccination by using an immunoglobulin M antibody capture enzyme-linked immunosorbent assay. J Clin Microbiol. 1992 Feb;30(2):346–350. doi: 10.1128/jcm.30.2.346-350.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  10. McFerran J. B., Dow C. Studies on immunisation of pigs with the Bartha strain of Aujeszky's disease virus. Res Vet Sci. 1975 Jul;19(1):17–22. [PubMed] [Google Scholar]
  11. Moormann R. J., de Rover T., Briaire J., Peeters B. P., Gielkens A. L., van Oirschot J. T. Inactivation of the thymidine kinase gene of a gI deletion mutant of pseudorabies virus generates a safe but still highly immunogenic vaccine strain. J Gen Virol. 1990 Jul;71(Pt 7):1591–1595. doi: 10.1099/0022-1317-71-7-1591. [DOI] [PubMed] [Google Scholar]
  12. Rodák L., Smíd B., Valícek L., Jurák E. Four-layer enzyme immunoassay (EIA) detection of differences in IgG, IgM and IgA antibody response to Aujeszky's disease virus in infected and vaccinated pigs. Vet Microbiol. 1987 Feb;13(2):121–133. doi: 10.1016/0378-1135(87)90037-x. [DOI] [PubMed] [Google Scholar]
  13. Westenbrink F., Kimman T. G. Immunoglobulin M-specific enzyme-linked immunosorbent assay for serodiagnosis of bovine respiratory syncytial virus infections. Am J Vet Res. 1987 Jul;48(7):1132–1137. [PubMed] [Google Scholar]
  14. van Oirschot J. T., Daus F., Kimman T. G., van Zaane D. Antibody response to glycoprotein I in maternally immune pigs exposed to a mildly virulent strain of pseudorabies virus. Am J Vet Res. 1991 Nov;52(11):1788–1793. [PubMed] [Google Scholar]
  15. van Oirschot J. T. Induction of antibodies to glycoprotein I in pigs exposed to different doses of a mildly virulent strain of Aujeszky's disease virus. Vet Rec. 1988 Jun 18;122(25):599–603. doi: 10.1136/vr.122.25.599. [DOI] [PubMed] [Google Scholar]
  16. van Oirschot J. T., Wijsmuller J. M., de Waal C. A., van Lith P. M. A novel concept for the control of Aujeszky's disease: experiences in two vaccinated pig herds. Vet Rec. 1990 Feb 17;126(7):159–163. [PubMed] [Google Scholar]

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