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. 1993 Sep;31(9):2315–2319. doi: 10.1128/jcm.31.9.2315-2319.1993

Rapid competitive enzyme-linked immunosorbent assay using a monoclonal antibody reacting with a 15-kilodalton tegumental antigen of Schistosoma mansoni for serodiagnosis of schistosomiasis.

A J Da Silva 1, M R Piuvezam 1, H de Moura 1, S Maddison 1, J M Peralta 1
PMCID: PMC265753  PMID: 8408548

Abstract

A competitive enzyme-linked immunosorbent assay (CELISA) for antibody detection was developed by using a monoclonal antibody which reacts with a 15-kDa tegumental antigen of the adult worm of Schistosoma mansoni. This monoclonal antibody was not able to react with antigens of Schistosoma japonicum or Schistosoma haematobium in enzyme-linked immunoelectrotransfer blot (EITB) and indirect immunofluorescence tests. The assay was performed in a period of 1 h using an adult worm crude extract antigen. To evaluate the CELISA, a total of 73 serum samples was analyzed: 35 were from S. mansoni-infected patients, 23 were from individuals with parasitic infections other than schistosomiasis, and 14 were from healthy individuals. All serum samples from healthy individuals and from patients infected with other parasites were negative, as were two (6%) samples from patients infected with S. mansoni. EITB analysis showed that 32 of 33 CELISA-positive samples were positive in the EITB but with different patterns of reactivity. A 15-kDa protein reacted with 60% of serum samples, and a 60-kDa protein showed the highest level of reactivity (85%). The two samples from patients infected with S. mansoni that were negative in the CELISA reacted with 70-, 60-, 50-, 47-, and 38-kDa proteins. One sample, positive in CELISA, did not react with proteins of the antigenic extract.

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Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Allan J. C., Avila G., Garcia Noval J., Flisser A., Craig P. S. Immunodiagnosis of taeniasis by coproantigen detection. Parasitology. 1990 Dec;101(Pt 3):473–477. doi: 10.1017/s0031182000060686. [DOI] [PubMed] [Google Scholar]
  2. Cabrera Z., Parkhouse R. M., Forsyth K., Gomez Priego A., Pabon R., Yarzabal L. Specific detection of human antibodies to Onchocerca volvulus. Trop Med Parasitol. 1989 Dec;40(4):454–459. [PubMed] [Google Scholar]
  3. Chappell C. L., Hackel J., Davis A. H. Cloned Schistosoma mansoni proteinase (hemoglobinase) as a putative serodiagnostic reagent. J Clin Microbiol. 1989 Jan;27(1):196–198. doi: 10.1128/jcm.27.1.196-198.1989. [DOI] [PMC free article] [PubMed] [Google Scholar]
  4. Correa-Oliveira R., Dusse L. M., Viana I. R., Colley D. G., Santos Carvalho O., Gazzinelli G. Human antibody responses against schistosomal antigens. I. Antibodies from patients with Ancylostoma, Ascaris lumbricoides or Schistosoma mansoni infections react with schistosome antigens. Am J Trop Med Hyg. 1988 Mar;38(2):348–355. [PubMed] [Google Scholar]
  5. De Jonge N., Gryseels B., Hilberath G. W., Polderman A. M., Deelder A. M. Detection of circulating anodic antigen by ELISA for seroepidemiology of schistosomiasis mansoni. Trans R Soc Trop Med Hyg. 1988;82(4):591–594. doi: 10.1016/0035-9203(88)90523-8. [DOI] [PubMed] [Google Scholar]
  6. De Jonge N., Rabello A. L., Krijger F. W., Kremsner P. G., Rocha R. S., Katz N., Deelder A. M. Levels of the schistosome circulating anodic and cathodic antigens in serum of schistosomiasis patients from Brazil. Trans R Soc Trop Med Hyg. 1991 Nov-Dec;85(6):756–759. doi: 10.1016/0035-9203(91)90446-6. [DOI] [PubMed] [Google Scholar]
  7. Deelder A. M., De Jonge N., Boerman O. C., Fillié Y. E., Hilberath G. W., Rotmans J. P., Gerritse M. J., Schut D. W. Sensitive determination of circulating anodic antigen in Schistosoma mansoni infected individuals by an enzyme-linked immunosorbent assay using monoclonal antibodies. Am J Trop Med Hyg. 1989 Mar;40(3):268–272. doi: 10.4269/ajtmh.1989.40.268. [DOI] [PubMed] [Google Scholar]
  8. Deplazes P., Eckert J., Pawlowski Z. S., Machowska L., Gottstein B. An enzyme-linked immunosorbent assay for diagnostic detection of Taenia saginata copro-antigens in humans. Trans R Soc Trop Med Hyg. 1991 May-Jun;85(3):391–396. doi: 10.1016/0035-9203(91)90302-f. [DOI] [PubMed] [Google Scholar]
  9. Doenhoff M. J., Dunne D. W., Lillywhite J. E. Serology of Schistosoma mansoni infections after chemotherapy. Trans R Soc Trop Med Hyg. 1989 Mar-Apr;83(2):237–238. doi: 10.1016/0035-9203(89)90658-5. [DOI] [PubMed] [Google Scholar]
  10. Fu C., Carter C. E. Detection of a circulating antigen in human schistosomiasis japonica using a monoclonal antibody. Am J Trop Med Hyg. 1990 Apr;42(4):347–351. doi: 10.4269/ajtmh.1990.42.347. [DOI] [PubMed] [Google Scholar]
  11. Gómez-Priego A., Godínez-Hana A. L., Gutiérrez-Quiroz M. Detection of serum antibodies in human Hymenolepis infection by enzyme immunoassay. Trans R Soc Trop Med Hyg. 1991 Sep-Oct;85(5):645–647. doi: 10.1016/0035-9203(91)90377-b. [DOI] [PubMed] [Google Scholar]
  12. Hancock K., Tsang V. C. Development and optimization of the FAST-ELISA for detecting antibodies to Schistosoma mansoni. J Immunol Methods. 1986 Sep 27;92(2):167–176. doi: 10.1016/0022-1759(86)90162-6. [DOI] [PubMed] [Google Scholar]
  13. Hayunga E. G., Möllegård I., Duncan J. F., Sumner M. P., Stek M., Jr, Hunter K. W., Jr Development of circulating antigen assay for rapid detection of acute schistosomiasis. Lancet. 1986 Sep 27;2(8509):716–718. doi: 10.1016/s0140-6736(86)90232-1. [DOI] [PubMed] [Google Scholar]
  14. Idris M. A., Ruppel A. Diagnostic Mr31/32,000 Schistosoma mansoni proteins (Sm31/32): reaction with sera from Sudanese patients infected with S. mansoni or S. haematobium. J Helminthol. 1988 Jun;62(2):95–101. doi: 10.1017/s0022149x00011305. [DOI] [PubMed] [Google Scholar]
  15. Janitschke K., El-Kalouby A. H., Braun-Munzinger R. A., El-Baz H., Mahmoud M. Evaluation of the ELISA test as an epidemiological tool in schistosomiasis. J Trop Med Hyg. 1981 Aug;84(4):147–154. [PubMed] [Google Scholar]
  16. Katz N., Chaves A., Pellegrino J. A simple device for quantitative stool thick-smear technique in Schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo. 1972 Nov-Dec;14(6):397–400. [PubMed] [Google Scholar]
  17. Khalife J., Grzych J. M., Pierce R., Ameisen J. C., Schacht A. M., Gras-Masse H., Tartar A., Lecocq J. P., Capron A. Immunological crossreactivity between the human immunodeficiency virus type 1 virion infectivity factor and a 170-kD surface antigen of Schistosoma mansoni. J Exp Med. 1990 Sep 1;172(3):1001–1004. doi: 10.1084/jem.172.3.1001. [DOI] [PMC free article] [PubMed] [Google Scholar]
  18. Lewis S. A., Strand M. Characterization of proteins and immunogens released by adult Schistosoma mansoni. J Parasitol. 1991 Apr;77(2):263–271. [PubMed] [Google Scholar]
  19. Maddison S. E. The present status of serodiagnosis and seroepidemiology of schistosomiasis. Diagn Microbiol Infect Dis. 1987 Jun;7(2):93–105. doi: 10.1016/0732-8893(87)90026-5. [DOI] [PubMed] [Google Scholar]
  20. Mitchell G. F., Premier R. R., Garcia E. G., Hurrell J. G., Chandler H. M., Cruise K. M., Tapales F. P., Tiu W. U. Hybridoma antibody-based competitive ELISA in Schistosoma japonicum infection. Am J Trop Med Hyg. 1983 Jan;32(1):114–117. doi: 10.4269/ajtmh.1983.32.114. [DOI] [PubMed] [Google Scholar]
  21. Mitchell G. F. The Wellcome Trust lecture. Problems specific to parasite vaccines. Parasitology. 1989;98 (Suppl):S19–S28. doi: 10.1017/s003118200007222x. [DOI] [PubMed] [Google Scholar]
  22. Tsang V. C., Hancock K., Kelly M. A., Wilson B. C., Maddison S. E. Schistosoma mansoni adult microsomal antigens, a serologic reagent. II. Specificity of antibody responses to the S. mansoni microsomal antigen (MAMA). J Immunol. 1983 Mar;130(3):1366–1370. [PubMed] [Google Scholar]
  23. Tsang V. C., Peralta J. M., Simons A. R. Enzyme-linked immunoelectrotransfer blot techniques (EITB) for studying the specificities of antigens and antibodies separated by gel electrophoresis. Methods Enzymol. 1983;92:377–391. doi: 10.1016/0076-6879(83)92032-3. [DOI] [PubMed] [Google Scholar]
  24. Yogore M. G., Jr, Lewert R. M., Blas B. L. Seroepidemiology of schistosomiasis japonica by ELISA in the Philippines. III. Selective mass chemotherapy with praziquantel in a control program. Am J Trop Med Hyg. 1984 Sep;33(5):882–890. doi: 10.4269/ajtmh.1984.33.882. [DOI] [PubMed] [Google Scholar]
  25. de Jonge N., De Caluwé P., Hilberath G. W., Krijger F. W., Polderman A. M., Deelder A. M. Circulating anodic antigen levels in serum before and after chemotherapy with praziquantel in schistosomiasis mansoni. Trans R Soc Trop Med Hyg. 1989 May-Jun;83(3):368–372. doi: 10.1016/0035-9203(89)90507-5. [DOI] [PubMed] [Google Scholar]
  26. de Jonge N., Kremsner P. G., Krijger F. W., Schommer G., Fillié Y. E., Kornelis D., van Zeyl R. J., van Dam G. J., Feldmeier H., Deelder A. M. Detection of the schistosome circulating cathodic antigen by enzyme immunoassay using biotinylated monoclonal antibodies. Trans R Soc Trop Med Hyg. 1990 Nov-Dec;84(6):815–818. doi: 10.1016/0035-9203(90)90094-u. [DOI] [PubMed] [Google Scholar]

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