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. 1993 Sep;31(9):2410–2416. doi: 10.1128/jcm.31.9.2410-2416.1993

Detection and identification of Mycobacterium tuberculosis directly from sputum sediments by amplification of rRNA.

V Jonas 1, M J Alden 1, J I Curry 1, K Kamisango 1, C A Knott 1, R Lankford 1, J M Wolfe 1, D F Moore 1
PMCID: PMC265770  PMID: 8408564

Abstract

Seven hundred fifty-eight processed sputum sediments received for the diagnosis of tuberculosis or other mycobacterial infections were tested by utilizing a rRNA target amplification assay and traditional culture techniques. The results from the rRNA target amplification assay (Gen-Probe Amplified Mycobacterium Tuberculosis Direct Test), available in 5 h, were compared with the results from standard culture techniques held for 6 weeks. A total of 119 specimens (16%) were culture positive for Mycobacterium tuberculosis. Overall sensitivity, specificity, positive predictive value, and negative predictive value were 82, 99, 97, and 96%, respectively, for the Gen-Probe assay; 88, 100, 100, and 97%, respectively, for culture; and 53, 99.8, 99.6, and 91%, respectively, for fluorochrome stain. The Gen-Probe assay employs the isothermal enzymatic amplification of M. tuberculosis complex rRNA followed by detection of the amplicon with an acridinium ester-labeled DNA probe. This assay has the potential of reducing the time for diagnosis of tuberculosis to 1 day.

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Selected References

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