Abstract
The Pastorex Aspergillus antigen test for detection of Aspergillus galactomannan antigen in the sera of patients with invasive aspergillosis is used in many clinical laboratories. A serum sample contaminated with Penicillium chrysogenum gave a strongly positive reaction (1:128) which was heat stable, was not eliminated by pronase treatment, and was not detected by a normal rabbit globulin control. This observation was shown to be due to cross-reactions of the monoclonal antibody EB-A2 used by the kit with several airborne fungi likely to contaminate serum samples, including Penicillium chrysogenum, Cladosporium herbarum, Acremonium species, Alternaria alternata, Fusarium oxysporum, Wangiella dermatitidis, and Rhodotorula rubra.
Full text
PDF![2489](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5066/265782/824ad2cb09bf/jcm00021-0255.png)
![2490](https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5066/265782/7942bedd5231/jcm00021-0256.png)
Selected References
These references are in PubMed. This may not be the complete list of references from this article.
- Bennett J. E., Friedman M. M., Dupont B. Receptor-mediated clearance of Aspergillus galactomannan. J Infect Dis. 1987 May;155(5):1005–1010. doi: 10.1093/infdis/155.5.1005. [DOI] [PubMed] [Google Scholar]
- Bennett J. E. Rapid diagnosis of candidiasis and aspergillosis. Rev Infect Dis. 1987 Mar-Apr;9(2):398–402. doi: 10.1093/clinids/9.2.398. [DOI] [PubMed] [Google Scholar]
- Dupont B., Huber M., Kim S. J., Bennett J. E. Galactomannan antigenemia and antigenuria in aspergillosis: studies in patients and experimentally infected rabbits. J Infect Dis. 1987 Jan;155(1):1–11. doi: 10.1093/infdis/155.1.1. [DOI] [PubMed] [Google Scholar]
- Kappe R., Müller J. Rapid clearance of Candida albicans mannan antigens by liver and spleen in contrast to prolonged circulation of Cryptococcus neoformans antigens. J Clin Microbiol. 1991 Aug;29(8):1665–1669. doi: 10.1128/jcm.29.8.1665-1669.1991. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Knight F., Mackenzie D. W. Aspergillus antigen latex test for diagnosis of invasive aspergillosis. Lancet. 1992 Jan 18;339(8786):188–188. doi: 10.1016/0140-6736(92)90261-z. [DOI] [PubMed] [Google Scholar]
- Reiss E., Lehmann P. F. Galactomannan antigenemia in invasive aspergillosis. Infect Immun. 1979 Jul;25(1):357–365. doi: 10.1128/iai.25.1.357-365.1979. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Rogers T. R., Haynes K. A., Barnes R. A. Value of antigen detection in predicting invasive pulmonary aspergillosis. Lancet. 1990 Nov 17;336(8725):1210–1213. doi: 10.1016/0140-6736(90)92831-2. [DOI] [PubMed] [Google Scholar]
- Stynen D., Sarfati J., Goris A., Prévost M. C., Lesourd M., Kamphuis H., Darras V., Latgé J. P. Rat monoclonal antibodies against Aspergillus galactomannan. Infect Immun. 1992 Jun;60(6):2237–2245. doi: 10.1128/iai.60.6.2237-2245.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
- Van Cutsem J., Meulemans L., Van Gerven F., Stynen D. Detection of circulating galactomannan by Pastorex Aspergillus in experimental invasive aspergillosis. Mycoses. 1990 Feb;33(2):61–69. doi: 10.1111/myc.1990.33.2.61. [DOI] [PubMed] [Google Scholar]
- Warnock D. W., Foot A. B., Johnson E. M., Mitchell S. B., Cornish J. M., Oakhill A. Aspergillus antigen latex test for diagnosis of invasive aspergillosis. Lancet. 1991 Oct 19;338(8773):1023–1024. doi: 10.1016/0140-6736(91)91890-7. [DOI] [PubMed] [Google Scholar]
- de Repentigny L. Serological techniques for diagnosis of fungal infection. Eur J Clin Microbiol Infect Dis. 1989 Apr;8(4):362–375. doi: 10.1007/BF01963470. [DOI] [PubMed] [Google Scholar]