Skip to main content
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1993 Sep;31(9):2502–2505. doi: 10.1128/jcm.31.9.2502-2505.1993

Typing of Aspergillus species and Aspergillus fumigatus isolates by interrepeat polymerase chain reaction.

A van Belkum 1, W G Quint 1, B E de Pauw 1, W J Melchers 1, J F Meis 1
PMCID: PMC265786  PMID: 8408576

Abstract

Polymerase chain reaction amplification of repetitive DNA motifs allows discrimination of Aspergillus species. In a preliminary survey, the DNA fingerprints appear to be identical when isolates of Aspergillus fumigatus are compared. When a primer deduced from a prokaryotic repeat motif is used, Aspergillus fumigatus isolates originating from different patients or different anatomical locations can be typed individually.

Full text

PDF
2502

Images in this article

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Aufauvre-Brown A., Cohen J., Holden D. W. Use of randomly amplified polymorphic DNA markers to distinguish isolates of Aspergillus fumigatus. J Clin Microbiol. 1992 Nov;30(11):2991–2993. doi: 10.1128/jcm.30.11.2991-2993.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Boom R., Sol C. J., Salimans M. M., Jansen C. L., Wertheim-van Dillen P. M., van der Noordaa J. Rapid and simple method for purification of nucleic acids. J Clin Microbiol. 1990 Mar;28(3):495–503. doi: 10.1128/jcm.28.3.495-503.1990. [DOI] [PMC free article] [PubMed] [Google Scholar]
  3. Denning D. W., Clemons K. V., Hanson L. H., Stevens D. A. Restriction endonuclease analysis of total cellular DNA of Aspergillus fumigatus isolates of geographically and epidemiologically diverse origin. J Infect Dis. 1990 Nov;162(5):1151–1158. doi: 10.1093/infdis/162.5.1151. [DOI] [PubMed] [Google Scholar]
  4. Giesendorf B. A., van Belkum A., Koeken A., Stegeman H., Henkens M. H., van der Plas J., Goossens H., Niesters H. G., Quint W. G. Development of species-specific DNA probes for Campylobacter jejuni, Campylobacter coli, and Campylobacter lari by polymerase chain reaction fingerprinting. J Clin Microbiol. 1993 Jun;31(6):1541–1546. doi: 10.1128/jcm.31.6.1541-1546.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Graybill J. R. Future directions of antifungal chemotherapy. Clin Infect Dis. 1992 Mar;14 (Suppl 1):S170–S181. doi: 10.1093/clinids/14.supplement_1.s170. [DOI] [PubMed] [Google Scholar]
  6. Kusne S., Torre-Cisneros J., Mañez R., Irish W., Martin M., Fung J., Simmons R. L., Starzl T. E. Factors associated with invasive lung aspergillosis and the significance of positive Aspergillus culture after liver transplantation. J Infect Dis. 1992 Dec;166(6):1379–1383. doi: 10.1093/infdis/166.6.1379. [DOI] [PMC free article] [PubMed] [Google Scholar]
  7. Meis J. F., Donnelly J. P., Hoogkamp-Korstanje J. A., De Pauw B. E. Aspergillus fumigatus pneumonia in neutropenic patients during therapy with fluconazole for infection due to Candida species. Clin Infect Dis. 1993 May;16(5):734–735. doi: 10.1093/clind/16.5.734. [DOI] [PubMed] [Google Scholar]
  8. Niesters H. G., Goessens W. H., Meis J. F., Quint W. G. Rapid, polymerase chain reaction-based identification assays for Candida species. J Clin Microbiol. 1993 Apr;31(4):904–910. doi: 10.1128/jcm.31.4.904-910.1993. [DOI] [PMC free article] [PubMed] [Google Scholar]
  9. Talbot G. H., Huang A., Provencher M. Invasive aspergillus rhinosinusitis in patients with acute leukemia. Rev Infect Dis. 1991 Mar-Apr;13(2):219–232. doi: 10.1093/clinids/13.2.219. [DOI] [PubMed] [Google Scholar]
  10. Versalovic J., Koeuth T., Lupski J. R. Distribution of repetitive DNA sequences in eubacteria and application to fingerprinting of bacterial genomes. Nucleic Acids Res. 1991 Dec 25;19(24):6823–6831. doi: 10.1093/nar/19.24.6823. [DOI] [PMC free article] [PubMed] [Google Scholar]
  11. Warburton P. E., Willard H. F. PCR amplification of tandemly repeated DNA: analysis of intra- and interchromosomal sequence variation and homologous unequal crossing-over in human alpha satellite DNA. Nucleic Acids Res. 1992 Nov 25;20(22):6033–6042. doi: 10.1093/nar/20.22.6033. [DOI] [PMC free article] [PubMed] [Google Scholar]
  12. Warnock D. W., Foot A. B., Johnson E. M., Mitchell S. B., Cornish J. M., Oakhill A. Aspergillus antigen latex test for diagnosis of invasive aspergillosis. Lancet. 1991 Oct 19;338(8773):1023–1024. doi: 10.1016/0140-6736(91)91890-7. [DOI] [PubMed] [Google Scholar]
  13. Young R. C., Bennett J. E., Vogel C. L., Carbone P. P., DeVita V. T. Aspergillosis. The spectrum of the disease in 98 patients. Medicine (Baltimore) 1970 Mar;49(2):147–173. doi: 10.1097/00005792-197003000-00002. [DOI] [PubMed] [Google Scholar]
  14. van Belkum A., De Jonckheere J., Quint W. G. Genotyping Naegleria spp. and Naegleria fowleri isolates by interrepeat polymerase chain reaction. J Clin Microbiol. 1992 Oct;30(10):2595–2598. doi: 10.1128/jcm.30.10.2595-2598.1992. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES