Skip to main content
NCBI home page
Journal of Clinical Microbiology logoLink to Journal of Clinical Microbiology
. 1987 Jan;25(1):76–80. doi: 10.1128/jcm.25.1.76-80.1987

Production, characterization, and species specificity of five monoclonal antibodies to Mycobacterium tuberculosis.

G V Kadival, S D Chaparas
PMCID: PMC265827  PMID: 3098780

Abstract

The production and characterization of five monoclonal antibodies to Mycobacterium tuberculosis are described. Specificity of the monoclonal antibodies was tested against other mycobacterial species by an enzyme-linked immunosorbent assay and immunoblots. HGT 3a, an immunoglobulin M (IgM) antibody, recognizes a molecule of 38,000 molecular weight present only in the tuberculosis complex of M. tuberculosis and Mycobacterium bovis BCG. HGT 6, an IgG1 antibody, recognized two molecules with molecular weights of 43,000 and 45,000 and showed limited cross-reactivity. Three other antibodies, HGT 1, HGT 2, and HGT 4, all belonging to the IgG1 type, recognized multiple bands and showed broad reactivity among all mycobacterial antigens tested, Escherichia coli and Nocardia asteroides.

Full text

PDF
76

Images in this article

77Image
on p.77
77Image
on p.77
78Image
on p.78
78Image
on p.78
78Image
on p.78
78Image
on p.78

Selected References

These references are in PubMed. This may not be the complete list of references from this article.

  1. Andersen A. B., Yuan Z. L., Hasløv K., Vergmann B., Bennedsen J. Interspecies reactivity of five monoclonal antibodies to Mycobacterium tuberculosis as examined by immunoblotting and enzyme-linked immunosorbent assay. J Clin Microbiol. 1986 Mar;23(3):446–451. doi: 10.1128/jcm.23.3.446-451.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  2. Chaparas S. D., Brown T. M., Hyman I. S. Antigenic relationships of various mycobacterial species with Mycobacterium tuberculosis. Am Rev Respir Dis. 1978 Jun;117(6):1091–1097. doi: 10.1164/arrd.1978.117.6.1091. [DOI] [PubMed] [Google Scholar]
  3. Coates A. R., Hewitt J., Allen B. W., Ivanyi J., Mitchison D. A. Antigenic diversity of Mycobacterium tuberculosis and Mycobacterium bovis detected by means of monoclonal antibodies. Lancet. 1981 Jul 25;2(8239):167–169. doi: 10.1016/s0140-6736(81)90355-x. [DOI] [PubMed] [Google Scholar]
  4. Daniel T. M., Gonchoroff N. J., Katzmann J. A., Olds G. R. Specificity of Mycobacterium tuberculosis antigen 5 determined with mouse monoclonal antibodies. Infect Immun. 1984 Jul;45(1):52–55. doi: 10.1128/iai.45.1.52-55.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  5. Gillis T. P., Buchanan T. M. Production and partial characterization of monoclonal antibodies to Mycobacterium leprae. Infect Immun. 1982 Jul;37(1):172–178. doi: 10.1128/iai.37.1.172-178.1982. [DOI] [PMC free article] [PubMed] [Google Scholar]
  6. Ivanyi J., Krambovitis E., Keen M. Evaluation of a monoclonal antibody (TB72) based serological test for tuberculosis. Clin Exp Immunol. 1983 Nov;54(2):337–345. [PMC free article] [PubMed] [Google Scholar]
  7. Kadival G. V., Mazarelo T. B., Chaparas S. D. Sensitivity and specificity of enzyme-linked immunosorbent assay in the detection of antigen in tuberculous meningitis cerebrospinal fluids. J Clin Microbiol. 1986 May;23(5):901–904. doi: 10.1128/jcm.23.5.901-904.1986. [DOI] [PMC free article] [PubMed] [Google Scholar]
  8. King J., Laemmli U. K. Polypeptides of the tail fibres of bacteriophage T4. J Mol Biol. 1971 Dec 28;62(3):465–477. doi: 10.1016/0022-2836(71)90148-3. [DOI] [PubMed] [Google Scholar]
  9. Kolk A. H., Ho M. L., Klatser P. R., Eggelte T. A., Kuijper S., de Jonge S., van Leeuwen J. Production and characterization of monoclonal antibodies to Mycobacterium tuberculosis, M. bovis (BCG) and M. leprae. Clin Exp Immunol. 1984 Dec;58(3):511–521. [PMC free article] [PubMed] [Google Scholar]
  10. Köhler G., Milstein C. Continuous cultures of fused cells secreting antibody of predefined specificity. Nature. 1975 Aug 7;256(5517):495–497. doi: 10.1038/256495a0. [DOI] [PubMed] [Google Scholar]
  11. LOWRY O. H., ROSEBROUGH N. J., FARR A. L., RANDALL R. J. Protein measurement with the Folin phenol reagent. J Biol Chem. 1951 Nov;193(1):265–275. [PubMed] [Google Scholar]
  12. Schou C., Yuan Z. L., Andersen A. B., Bennedsen J. Production and partial characterization of monoclonal hybridoma antibodies to Mycobacterium tuberculosis. Acta Pathol Microbiol Immunol Scand C. 1985 Dec;93(6):265–272. doi: 10.1111/j.1699-0463.1985.tb02955.x. [DOI] [PubMed] [Google Scholar]
  13. Young D. B., Khanolkar S. R., Barg L. L., Buchanan T. M. Generation and characterization of monoclonal antibodies to the phenolic glycolipid of Mycobacterium leprae. Infect Immun. 1984 Jan;43(1):183–188. doi: 10.1128/iai.43.1.183-188.1984. [DOI] [PMC free article] [PubMed] [Google Scholar]
  14. Young R. A., Bloom B. R., Grosskinsky C. M., Ivanyi J., Thomas D., Davis R. W. Dissection of Mycobacterium tuberculosis antigens using recombinant DNA. Proc Natl Acad Sci U S A. 1985 May;82(9):2583–2587. doi: 10.1073/pnas.82.9.2583. [DOI] [PMC free article] [PubMed] [Google Scholar]

Articles from Journal of Clinical Microbiology are provided here courtesy of American Society for Microbiology (ASM)

RESOURCES